“令AB的是C”类句式考察

对＂令AB的是C＂这类句式作了较为细致的考察。主要结论是：一、充当句式中B的词语,绝大多数都是表示心理状态的形容词或动词词语。二、从C的句法形式考察,存在着＂复杂式＂和＂简单式＂两种类型。但在出现频率上,＂复杂式＂比＂简单式＂高得多。其原因与绝大多数的C属于新信息,特别是与说话人的表达需要直接相关;三、以廖秋忠所提供的几种手段为依据来确定＂是＂的管界。结果表明,几种手段在适用频率上存在着很大的差异。     

不饱和类锗烯H_2CGeLiBr的理论研究

采用DFTB3LYP和QCISD方法研究了不饱和类锗烯H2C=GeLiBr的结构及异构化反应．结果表明，不饱和类锗烯H2C=GeLiBr有3种平衡构型，其中非平面的p-配合物型构型能量最低，是其存在的主要构型．对平衡构型间异构化反应的过渡态进行了计算，求得了转化势垒；计算模拟了最稳定构型的红外光谱．     

贵州南方菜豆花叶病毒的ELISA检疫

采用ELISA间接法对分布于贵州几个地区的菜豆进行南方菜豆花叶病毒的检测 ,结果表明 :在所采集到的样品中 ,仅贵阳地区的菜豆感染有南方菜豆花叶病毒。其检测的灵敏度和最佳反应稀释度分别为 80 6ng/mL和 1∶5 0 0稀释度。     

H2株甲肝减毒活疫苗K7的核苷酸全序列分析

应用ＲＴ－ＰＣＲ获取Ｈ２株甲肝减毒活疫苗（Ｋ７）的ｃＤＮＡ片段，经末端终止测序ＰＣＲ试剂盒和全自动测序仪（ＡＢＩ３７７）作序列测定，用Ｍａｘｔｒｉｇｅｎｅ软件进行计算机分析，Ｋ７疫苗病毒的核苷酸全长含７４４３个碱基．Ｋ７对比其亲代Ｈ２株，减毒的ＨＭ１７５株以及ＨＭ１７５野毒株，同源性分别为９９．７５％，９９．９５％和９９．６１％；彼此间在Ｐ１和Ｐ２连接区１６８个碱基的同源性为９９．４％～１００％，均为ⅠＢ基因亚型．前三者在５’非编码区均有１３１～１３４位和２０３位５个碱基缺失，且结构蛋白基因区序列完全一致．本文用分子病毒学方法证实了Ｋ７疫苗的优异纯毒水平，也为开展甲型肝炎分子流行病学工作提供了基础资料     

等离子体波动方程的摄动分析

分析了热等离子体中离子声孤子存在及传播的力学机制，并根据远方场简单波理论，采用摄动方法推导出了KdV方程。     

Thymidine kinase gene mutation leads to reduced virulence of pseudorabies virus

To explore correlation between the tk gene structure of pseudorabies virus (PRV) and its virulence, to study the effect of the gene mutation on PRV biological properties, and to investigate mechinism of reduced virulence, thymidine kinase (TK)-deficient mutant of pseudorabies virus strain Hubei (PRV HB) was isolated by selection for resistance to 5-bromodeoxyuridine. The tk genes of PRV HB and its TK^― mutant were cloned and sequenced. 1587 base pairs of the tk gene and flanking regions of wild-type (wt) virus were sequenced, which included an open reading frame (ORF) of 1098 bp encoding a protein of 366 amino acids. The ORF contained two 137-bp repeated sequences, which were connected by an adenosine. 1458 bp of the tk and flanking regions of TK^― mutant were sequenced. Analysis of the tk gene sequence of TK^― mutant indicated that one of 137 bp repeated sequence and the connecting adenosine in the tk gene of the wt virus was deleted and a repeated sequence of 8 nucleotides (GCGCGCC) was inserted. All other nucleotides of TK^―mutant were identical to that of wt virus. Deletion and insertion of the nucleotide sequence resulted in a frameshift and a premature chain termination, and the resultant TK protein was not active. Analysis of the amino acid sequence revealed that TK protein of PRV HB contained the conserved consensus sequence of herpesviral TKs and an additional conserved-DHR-motif. The results of this work also indicated that TK^― mutant was genetically stable. Compared to PRV HB, virulence of TK^― mutant was greatly decreased. Mice vaccinated with TK^― mutant were completely protected against a lethal challenge with virulent PRV (HB).     

6,7-dichlorobenzo[de]isochromene-1,3-dione的合成新方法

本文报道了6,7-dichlorobenzo[de]isochromene-1,3-dione的合成新方法。该路线简单易行,后处理方便,且产率较高,有较高的实用价值。     

预淬火对G Cr15钢M/ B复合组织及性能的影响

研究了预淬火马氏体对 Ｇ Ｃｒ１５ 钢马氏体／ 下贝氏体（ Ｍ／ Ｂ） 复合组织及性能的影响。试验结果表明，通过 Ｍｓ 以下适当温度的预淬火，获得的 Ｍ／ Ｂ 复合组织具有更优的机械性能。     

郑州帝湖花园物业收费管理系统的设计与实现

本系统是在郑州市帝湖花园实际手工物亚收费管理工作的基础上,结合基于B/S软件开发的特点,对其工作业务进行了较系统、全面的分析的基础上,编制出了郑州帝湖花园物业收费管理系统.本文对系统分析、系统功能、系统构成、数据库应用等做了较详的论述.