Surface molecules regulating rolling and adhesion to endothelium of neutrophil granulocytes and MDA-MB-468 breast carcinoma cells and their interaction

The extravasation of leukocytes and tumor cells is a multi-step process with the involvement of various adhesion molecules depending on the three steps rolling, adhesion, and diapedesis. We have developed an in vitro model, by which we investigated the rolling and adhesion of neutrophil granulocytes and MDA-MB-468 human breast carcinoma cells to lung endothelial cells under physiological flow-conditions. We found that norepinephrine had an inhibitory function on the fMLP-promoted adhesion of neutrophil granulocytes due to a down-regulation of β2-integrin. Furthermore, neutrophil granulocytes serve as linking cells for the interaction of the MDA-MB-468 cells with the endothelium, which are both β2-integrin negative, but express the β2-integrin ligand ICAM-1. In addition, we show here that N-cadherin is up-regulated on the endothelial cells and on neutrophil granulocytes in response to fMLP. This up-regulation resulted in a significant increase of adherent MDA-MB-468 cells, which are also N-cadherin positive. Received 3 September 2007; received after revision 17 October 2007; accepted 22 October 2007     

激光治疗肿瘤的计算研究

根据激光治疗肿瘤的机理建立了激光治疗肿瘤的理论计算模型,并应用有限元计算方法对双光纤照射椭球形肿瘤组织的模型进行了定量计算研究;研究结果表明,对于长轴为2.0 cm、短轴为1.2 cm的实心椭球形肿瘤体,将2个直径为0.4 mm的球状光纤对称放置在主轴上相距0.8~1.0 cm的范围内,可以使光动力学的效果和热疗的效果同时达到最佳值。     

骨桥蛋白在卵巢肿瘤组织的表达及其与恶性肿瘤侵袭转移的关系

目的:探讨骨桥蛋白(OPN)在卵巢肿瘤组织的表达及其与恶性肿瘤侵袭转移的关系。方法:采用免疫组织化学方法测定OPN在48例卵巢恶性肿瘤组织、8例卵巢临界恶性肿瘤组织、20例卵巢良性肿瘤组织及10例卵巢正常组织中的表达。结果:OPN在卵巢恶性肿瘤组织表达强度为0.485±0.134,显著高于卵巢正常组织及卵巢临界恶性及良性肿瘤组织表达强度(0.122±0.023,0.243±0.076及0.168±0.045,P<0.01);OPN的表达与恶性肿瘤的临床期别及分化程度相关,而与细胞组织学类型无关。结论:OPN在卵巢肿瘤的发生发展中起重要作用,并与卵巢恶性肿瘤的侵袭转移相关。     

Two new species of asterocherid (Copepoda: Siphonostomatoida) associated with a sponge from the Strait of Messina,Italy: taxonomic and ecological traits

Two new species of asterocherid associated with sponges are described from the Mediterranean Sea. They are Psilomyzon laetitiae sp. nov. and Psilomyzon sarcotragusicola sp. nov. The genus Psilomyzon consists of only one known species, P. pauciseta. The two new species can be easily distinguished from the cogener species by reduction of the endopod of leg 4. In P. pauciseta the leg 4 endopod is 3-segmented, with the third segment reduced; in both new species it is 2-segmented, with the second segment carrying an inner projection, possibly homologous to the third segment of the leg 4 endopod of P. pauciseta. In the two new species, the bifid outer process on male leg 4 second endopodal segment seems to be serially homologous to the similar processes on the same segment in legs 1–3, whereas the inner projection may represent a vestigial element of the third endopodal segment of leg 4.

http://zoobank.org/urn:lsid:zoobank.org:pub:33BF0653-BBA9-426B-B365-5D8C62DFD44A     

恶性肿瘤中IGFBP-rP1的抑癌基因作用机制及其可能的临床实用价值

胰岛素样生长因子结合蛋白相关蛋白1(IGFBP-rP1)是近年来恶性肿瘤的研究热点.本文主要综述IGFBP-rP1在恶性肿瘤中的抑癌基因作用机制及可能的临床实用价值.IGFBP-rP1在恶性肿瘤中的作用广泛涉及细胞的增殖、衰老、凋亡、分化、血管生成等多方面,研究指出IGFBP-rP1可缩短细胞增殖周期并影响非停泊性生长从而抑制增殖,降低致瘤能力;调节BRAF-MEKERK信号通路及pRB、HSP60等相关蛋白的表达从而影响衰老及凋亡;主要通过IGF依赖方式抑制血管生成;而且IGFBP-rP1表达下降跟肿瘤细胞分化程度降低有关.研究显示IGFBP-rP1有一定的临床实用价值,如其表达量跟恶性肿瘤的进展相关,低表达提示某些化疗药物抵抗,可提示预后.而在恶性肿瘤中特异性地上调IGFBP-rP1,可抑制肿瘤增殖及血管生成、诱导细胞衰老凋亡、提高肿瘤分化程度及化疗敏感性,具有治疗意义,但研究者们还在努力探究,争取早日找到一种临床有效的靶向IGFBP-rP1的基因治疗方法.     

铋基纳米材料在肿瘤诊治和抗菌中的应用进展

随着纳米技术的快速发展,纳米材料作为新型生物材料在生物医学领域表现出独特的优势,受到研究人员的广泛关注。铋基纳米材料因其良好的生物相容性和优异的光学等物理化学特性,在肿瘤诊治和抗菌等生物医学领域的应用已被广泛研究和报道,并展现出广阔的应用前景。简要综述了生物医用铋基纳米材料在计算机断层扫描成像、光声成像等生物成像和光动力治疗、放射治疗、光热治疗等肿瘤治疗以及抗菌中的研究进展,希望为铋基纳米材料在生物医学领域中的应用提供帮助。     

2402例肿瘤化疗患者医院感染分析及预防措施

目的了解肿瘤化疗患者医院感染的现状,并探讨预防医院感染的相关措施.方法用回顾性调查方法对2 402例肿瘤化疗住院患者进行医院感染监测.结果有87例肿瘤化疗患者发生医院感染,感染率为3.62%;感染部位以呼吸道感染最高,占75.86%;肿瘤类型以肺恶性肿瘤居首,占51.72%;检出的病原菌中以革兰阴性杆菌检出最高,占65...     

组织间和表面光动力治疗恶性肿瘤疗效分析

运用激光-血卟啉光动力效应治疗恶性肿瘤49例,总有效率达87.8％。其中运用组织间照射方法治疗的显效率达100％,完全效应者占 92.3％。应用表面照射方法治疗的显效率为 47. 8％,完全效应者仅占 17.4％。说明不同照光方法直接影响治疗效果。     

视网膜母细胞瘤基因产物磷酸化水平与细胞生长状态的关系

随着无血清培养细胞时间的延长，红胞合成Ｒｂ蛋白量和磷酸化水平逐渐降低，当细胞生长停止时，Ｒｂ蛋白处于低磷外化状态．正丁酸钠可诱导ＨＬ６０终端分化为单核－巨噬细胞；视黄酸、二甲基亚砜使ＨＬ６０分化为粒细胞．尽管分化途径不同．但是终端分化的细胞都只能合成低磷酸化的Ｒｂ蛋白．结果表明当细胞处于生长停滞状态，细胞都只能合成低磷酸化的Ｒｂ蛋白．