Sequence analysis of a 7.3 kb Bam H Ⅰ genomic fragment of fowlpox virus strain 282E_4

A 7.3 kb BamH I genomic fragment from fowlpox virus (FPV) strain 282E4 has been sequenced. After comparison with the FPV sequences collected in Genbank, and the entire genomic sequence of vaccinia virus (VV) strain Compenhagen, a 11.2 kb BamH I fragment from FPV strain Munich HP-438 has been found homologous to the fragment. Every predicted open reading frame (ORF) within the fragment has been compared with the ORFs of W strain Copenhagen at the amino acid level. The signal peptide and transmembrane region of each peptide encoded by each corresponding ORF predicted in the fragment have been studied as well.     

Oxalate-induced resistance of muskmelon to WMV-2

The treatment of muskmelon cultivar “Wangwenxiang”, which is sensitive to watermelon mosaic virus-2 (WMV-2), with oxalate increases systemic resistance of the cultivar to WMV-2 significantly. The challenge inoculation experiments indicate that the symptom of oxalate-treated plant is much slighter than that of control plants and the virus content is only 4% of that of control plants. The peroxidase activity of oxalate-treated plant is six times of that of control plants; three new isoperoxidases are induced and lignin content increases by 82.9%. These results indicate that oxalate induces systemic resistance of muskmelon to WMV-2 while it induces increase of peroxidase activity.     

Construction of transposon-mediated baculovirus vector and expression of green fluorescent protein in insect cells and larvae

A transposon-shuttle vector Hanpvid was constructed by using wild-type genomic DNA fromHeliothis armigera nuclear polyhedrosis virus (HaNPV). It could replicate inE. coli cells as a large plasmid and remain infectious when being induced into insect cells. Hanpvid comprises HaNPV DNA and a transposon cassette which includes a miniF replicon, a kanamycin resistance gene (kan), lacZa and an attachment site for Tn7 (attTn7). Recombinant virus rHa-FaGP was obtained after transposition of a donor plasmid carrying green fluorescent protein gene (gfp) and polyhedrin gene (ocu) into attTn7. SDS-PAGE analysis shows that both gfp and ocu genes were highly expressed inHeliothis armigera cells. Green Hemolymphocytes can be seen under a fluorescent microscope 4 d after recombinant virus rHa-FaGP infected the third-instar larvae. The infected larvae show strong green fluorescence 6 d post infection.     

Molecular dynamics simulation of site-directed mutagenesis of HIV-1 Tattrans-activator

The Cys-rich domain, core region and basic domain are highly conserved and very important to thetrans-activation activity of HIV-1 Tattrans-activator. The three-dimensional structures of 6 mutants of HIV-1 Tat protein were constructed with the methods of molecular dynamics simulation. The variations of the structures of the mutants have been analyzed and the factors that led to abolishment oftrans-activation activity have been discussed.     

丙型肝炎病毒DNA免疫研究进展

DNA免疫是近年发展起来的一种新型免疫技术。由于其具有显的优越性,特别适用于具有高度变异性的丙型肝炎病毒(HCV)疫苗的研制。对丙型肝炎病毒DNA免疫的研究证明,确可诱生较高水平的体液及细胞免疫应答。     

日本脑炎病毒(JEV)prME的克隆及其植物表达载体构建

为研究乙脑亚单位口服疫苗,根据Genbank已发表的日本脑炎病毒（JEV）prME基因序列,设计引物,PCR扩增出5锚含有Kozak box,3端含有SEKDEL编码区的prME,克隆入pMD18-T载体中,经过序列测定后,用SnaBI和BamHI酶切消化将prME克隆入用EcoICRI和BamHI酶切消化的质粒pBI121 CaMV35S启动子和NOS终止子之间形成一个表达盒,再用HindⅢ,EcoRI酶切消化将该表达盒克隆入相同酶切的质粒pCAMBIA1301中,获得含有prME的植物双元表达载体．     

上网安全

本文分析上网涉及的安全问题 ,介绍了关于密码安全、黑客攻击、病毒破坏诸方面的预防措施 .     

校园网络病毒防治及补丁自动升级系统研究

相比传统的主机病毒,在开放式网络环境中网络病毒疫情的蔓延更加迅速,破坏力也更强．针对Windows系统补丁数量大、种类多的特点,研究如何在校园网内部部署病毒自动更新系统及补丁自动升级系统,保障校园网络系统安全运行．     

生物粉末活性炭微过滤法对废水回收的利用

基于对生物粉末活性炭(BPAC)微过滤(MF)系统在废水回收和回用中作为非传统工艺的研究作了评估．系统在活性炭质量浓度为20g／L,水温25℃,跨膜压力为55kPa的条件下运行．发现有机去除主要在膜组件发生,这是由粉末活性炭在膜组件发生的累积引起．平均有机去除率为83％,致使出水TOC质量浓度在1～2mg／L．在水温为15℃下该工艺的运行效果没有变差,此时有机去出率为89．6％．认为较低水温时的高有机去除率主要是因为通过提升跨膜压力至82kPa使膜的渗流速度保持在同一大小,微生物的自身分解减少．BPAC—MF系统对细菌的去除明显．从研究状况下的物料平衡来看,99．9997％的进水大肠杆菌Qβ从系统中去除．     

基于二维码手机的烟草公司业务管理系统的设计与实现

设计了一个烟草公司业务管理系统,该设计集二维码、二维码手机、GPRS网络、WEBSERVICES技术于一体,实现了无线实时数据采集和数据分析,解决了数据采集和汇总问题,满足了用户急需的及时、完整的数据管理,通过对实时的数据分析实现业务控制、管理和决策支持.