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91.
研究了B2型金属间化合物 FeAl在机械球磨过程中的嵌镶碎块尺寸与长程有序度之间的关系。发现随球磨时间的增长,嵌镶碎块尺寸和长程有序度同时减小。由于嵌镶碎化主要由位错亚晶粒造成,应用Cottrell气团模型可建立嵌镶碎块尺寸与长程有序度之间的关系式。所得的这个关系式表明,长程有序度的降低主要是由位错与点阵原子的相互作用引起的。 相似文献
92.
大袋蛾核型多角体病毒,病状及毒力的生物测定 总被引:1,自引:0,他引:1
赵博光 《南京林业大学学报(自然科学版)》1993,17(4):22-26
报道了对大袋蛾核型多角体病毒、病状的观察。林间因感染大袋蛾核型多角体病毒而死亡的幼虫可分为两类:一类为纯病毒感染,另一类为病毒和细菌混合染。用不同浓度的大袋蛾核型多角体病毒感染大袋蛾卵,得多角体浓度与死亡率的直线回归方程:y=0.78+0.823x,LC50为1.18×10^5PIB/mL。用10^5,5×10^5,10^6 PIB/mL感染卵,得感染时间与死亡率的关系和LT50,分别为:y=6. 相似文献
93.
王战 《西北大学学报(自然科学版)》1996,26(5):439-444
论波浪镶嵌学说的自然观——谨以此文纪念张伯声院士逝世两周年@王战... 相似文献
95.
96.
HE Hongjun CAO Sihua LUO Li FENG Tao PAN Li ZOU Zhiji 《武汉大学学报:自然科学英文版》2006,11(6):1697-1700
Security experts have not formally defined the distinction between viruses and normal programs. The paper takes user's intension as the criteria for malice, gives a formal definition of viruses that aim at stealing or destroying files, and proposes an algorithm to detect virus correctly. Compared with traditional definitions, this new definition is easy to understand, covers more malwares, adapts development of virus technology, and defines virus on the spot. The paper has also analyzed more than 250 real viruses and finds that they are all in the domain of the new definition, this implies that the new definition has great practical significance. 相似文献
97.
ZHOUHantao LINQingtong PANWen GAOYuanyuan CHENPan CHENXu LIUBo 《科学通报(英文版)》2004,49(5):456-461
Salt-tolerant gene, CSRG1, which was isolated from a kind of salt-tolerant mangroves, Avicennia marina, constructed the transgenic plasmid, pGAM189/CSRG1. CSRG1, GUS, Kmr and Hyg^r could be transferred into tobacco genome by the ameliorated leaf discs method of agro-bacterium-mediate transformation. Thirteen stable resistant lines were obtained when fifty transgenic explants were selected through 50 mg/L hygromycin and 150 mg/L kanamycin. Assessments of PCR amplification, Southern blot analysis and GUS histochemical staining showed that CSRG1 has been integrated into the genome of the eleven transgenic lines (frequency of transformation was 22%). Northern bolt analysis revealed that CSRG1 had expressed in transgenic lines. The assessments of salt-tolerant ability and photosyn-thetic rates indicated that the survival rate of the transgenic lines is 80%—90% and the transgenic lines could increase by 30%—40% in plant height, even when they were cultivated in MS medium containing 2% NaCl and the total seawater (salinity 24). It is supposed that the special physiologic metabolic pathway formed by the products of CSRG1 can really endow the tobacco plants with the high salt-tolerant ability, not only to Na^ stress, but also to the comprehensive stress of various ions. 相似文献
98.
LIUGuangqing LIUZaixin XIEQingge CHENYingli BAOHuifang LIUXiangtao 《科学通报(英文版)》2004,49(11):1137-1141
The China foot-and-mouth virus (FMDV) isolate OH/CHA/99 was isolated from swine, which was unable to infect bovine thyroid cells in vitro or to cause typical disease in bovines following intradermal inoculation in the tongue. To enhance antigenicity, replication, maturation and pathogenicity studies of OH/CHA/99, an infectious fulllength cDNA clone, designated pBIFMDV, was prepared. The in vitro and in vivo biological properties of the virus derived from pBIFMDV were studied by analyzing antigenicity, plaque morphology and virulence in pigs. The results showed that the virus derived from pBIFMDV had the same biological properties as the parent strain OH/CHA/99; the fulllength infections cDNA clone, pBIFMDV, will be very useful in studies of the antigenicity, virulence, pathogenesis, maturation and replication of FMDV. 相似文献
99.
Immunogenicity of recombinant fowl-pox virus co-expressing structural protein precursor P1-2A and proteinase 3C of FMDV 总被引:1,自引:0,他引:1
JINNingyi ZHANGHongyong YINGefeng ZHENGMin LIUTong JIANGWenzheng LIZijian 《科学通报(英文版)》2004,49(8):823-823
Two recombinant plasmids, pUTA2P1 and pUTAL3CP1, were constructed by inserting structural protein precursor P1-2A and proteinase 3C of foot-and-mouth disease virus (FMDV) into fowl-pox virus (FPV) recombinant vectors pUTA-2 and pUTA-16-LacZ respectively, and two recombinant FPVs (vUTA2P1 and vUTAL3CP1) screened by the RT-PCR, IFA assay and Western blotting assay were obtained successfully. Mice injected respectively with rFPVs were induced high level specific anti-FMDV antibodies, increasing of T subtypes, and higher cytotoxicities of splenocytes than those of control groups. These results indicated that a new method was used to construct a potential candidate vaccine of FMDV. 相似文献
100.
CUIXiaofeng ZHOUXueping 《科学通报(英文版)》2004,49(24):2607-2612
Tomato yellow leaf curl China virus Y10 isolate (TYLCCNV-Y10) alone could systemically infect host plants such as Nicotiana benthamiana without symptoms. In contrast, Tobacco curly shoot virus Y35 isolate (TbCSV-Y35) alone induces leaf curl symptoms in N. benthamiana. When inoculated into transgenic N. benthamiana plants expressing GFP gene (line 16c), TYLCCNV-Y10 neither reverses the established GFP silencing nor blocks the onset of GFP silencing. In contrast, TbCSV-Y35 can partially reverse the established GFP silencing and block the onset of GFP silencing in new leaves. In the patch co-infiltration assays, the AC2 and AC4 proteins of TYLCCNV-Y10 and TbCSV-Y35 could suppress local GFP silencing and delay systemic GFP silencing, suggesting that they are suppressors of RNA silencing. Comparison of the accumulation levels of GFP mRNA in the co-infiltration patches showed that Y10 AC2 and Y35 AC2 proteins had similar efficiency for suppression of RNA silencing. However, Y35 AC4 protein functioned as a stronger suppressor of RNA silencing than Y10 AC4 protein. There-fore, the pathogenicity difference between TbCSV-Y35 and TYLCCNV-Y10 may be related to the functional difference in their AC4 proteins. 相似文献