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871.
采用玻片免疫酶法(IEA)和酶联免疫吸附试验法(ELISA),检查了来自广西不同来源恒河猴B病毒的相关抗体。结果表明:广西野生恒河猴受B病毒的感染较为普遍,来自龙虎山及扶绥保护区的猴,B病毒相关抗体阳性率分别是79.7%和75.3%。来自穿洞河及布柳河保护区的猴,B病毒相关抗体阳性率分别是26.1%和28.9%。大新保护区猴阳性率为57.1%。在不同组猴中,小群关养组猴相关抗体阳性率最高,野生猴次之,自繁猴最低。三组间,猴B病毒相关抗体阳性率的差异极显著(P<0.05),经ELISA检查确定为阴性的猴,用IEA检查出7.5%(15/200)的猴被判定为阳性猴 相似文献
872.
为了探讨丙型肝炎病毒(HCV)感染与肝细胞癌(HCC)的关系以及HCV可能的致癌机理,采用免疫组织化学方法及巢式PCR法检测了136例肝细胞癌等肝病组织中的HCVNS3抗原、HCVRNA及P21、P53蛋白。结果表明,肝细胞癌及癌周肝组织中有HCVNS3抗原及HCVRNA检出,支持HCV与HCC的关联。P21在HCC、肝炎后肝硬化、慢性肝炎、体质性黄疸各组中的检出率随病变的加重而逐渐增高,在HCC的癌及癌周组织中P21呈致密的过量表达,提示ras癌基因的激活在HCC的发生过程中起一定作用。P53的阳性率较P21低,但p53的突变似乎也是肝癌发生的协同因素之一。组织中P21的过量表达与HCVNS3抗原阳性检出呈正相关,HCVNS3抗原与P21的这种关联提示,HCV感染作为HCC的密切相关因素之一,可能通过激活某些癌基因或使某些抑癌基因突变而致肝细胞癌变 相似文献
873.
应用免疫吸附电镜检测甘薯羽状斑驳病毒 总被引:3,自引:0,他引:3
应用血清学特异性的免疫吸附电镜法对接种SPFMV的I.setosa、I.nil和网室种植的感染SPFMV的徐薯-18、新大紫甘薯叶片分别进行了测定.结果表明被检测的四种植物汁液的稀释度分别可达到1/1280、1/640、1/640和1/160;而用无血清包被的电镜铜网检测I.setosa、I.nil和徐薯-18,其汁液稀释度仅达到1/20.比较接种SPFMV的I.setosa和I.nil植物体内病毒含量,前者高于后者.网室种植的自然感染SPFMV的两种甘薯品种,体内病毒含量也不一样. 相似文献
874.
胡廷章 《四川师范大学学报(自然科学版)》1996,19(6):105-106
改进了芜菁花叶病毒的分离纯化方法,避免了分离纯化芜菁花叶病毒过程中通常遇到的病毒粒子聚集的现象,提高了病毒的得率,这为研究芜菁花叶病毒提供了有效的分离纯化方法。 相似文献
875.
876.
棉铃虫单粒包埋型核型多角体病毒(HaSNPV)能够诱发草地贪夜蛾Sf细胞株发生早期死亡现象.依据死亡过程的细胞形态变化,DNA降解的梯状电泳特征以及对不同抑制剂敏感性的差异,可以初步断定该死亡现象为程序性死亡.早期死亡伴随着病毒DNA复制与子代病毒生成的中止.同时发现野生型ACMNPV的共感染能够有效地抑制早期死亡的发生,并对抑制作用的可能性机制进行了探讨. 相似文献
877.
在叙述虚拟还原技术的原理后,主要探讨了两个问题:还原卡和还原软件,这种被广泛运用在校园网和学校公共机房的技术其保护机理是如何实现的;是否存在一种代码可以穿透虚拟还原技术的保护。 相似文献
878.
By fusing seed-specific promoter nap300 with β-glucuronidase gene, it was found that this about 300bp DNA fragment was sufficient to direct seed-specific gene expression. The substitution mutation in both distB and proxB elements had a little effect on the expression efficiency and almost no effect on the organ-specific expression pattern. In the experiment designed to compare nap300 with 7S promoter, the result showed that tissue specificity for nap300 was higher than that for 7S, and its expression level was lower than 7S's. There was no big difference in their expression pattern, and the maximal activity stage for the two promoters was identical, which indicated they could be used simultaneously for expressing different foreign genes in seeds. 相似文献
879.
The measles virus (MV) strains with mutated hemagglutinin gene (ha) lost the capacity to infect its sensitive host cells (Vero cells), but it may infect the marmoset B-lymphoblastoid cell line B95a. From above, we can presume that there is a novel cellular receptor for those measles virus strains on B95a cell s. Using the yeast two-hybrid system, we screened and cloned a novel gene--bip (B-lympho- blastoid interaction protein of marmoset) from B95a cell cDNA library, which encoded a protein interacting with measles virus hemagglutinin protein (Ha). The bip cDNA was 1540 base pairs in length and contained a unique open rea ding frame (ORF) of 1011 base pairs encoding a transmembrane protein of 337 amino acid residues. The primary structure of amino acids residue is predicted that the Bip comprised a hydrophobic transmembrane domain and a hydrophobic leader region. The researches about the deletion mutants showed that the deletion of tran smembrane domain in Bip did not affect the interaction between Bip and Ha protei ns. Expression of bip in measles virus non-permissive cell line--CHO (Chinese hamster ovary) cells was performed to prove that CHO/Bip can be infected by meas les virus and then turned to the MV permissive cells. We concluded that the bip gene is a novel measles virus receptor gene in marmoset B-lymphoblastoid cells. 相似文献
880.
The complete sequence of an Allexivirus isolated from garlic plants in Yuhang City, Zhejiang Province, China had been determined. The single-strand, positive RNA
genome was 8451 nucleotides in length excluding poly(A) tail. The genome organization of this virus was similar to that of
the other Allexiviruses but only with 62.8%–64.8% nucleotide acid identities. The amino acid sequences of proteins encoded
by ORF1-6 shared 67.6%–78.5%, 55.4%–66.2%, 56.7%–66.4%,40.3%–55.6%,66.3%–79.7%and 52.2%–68.8% identities with those of the
others respectively. The homology range between it and the other Allexiviruses was similar to that between the other distinct
species in this genus. A more comprehensive comparison using all available CP amino acid sequences showed that it shared only
63.9%–79.8% amino acids identical with the others. Therefore, it had been considered as a new member of the genus, named as
garlic virus E (GarV-E). Phylogenetic analysis confirmed GarV-E as a distinct member and the correct names and classification
of some members of genus Allexivirus were also discussed. 相似文献