化学通讯介导对中缅树鼩个体识别的影响

为了探究尿液、粪便及胸腺分泌物对中缅树鼩个体识别的影响,通过Y-迷宫对中缅树鼩尿液、粪便和胸腺分泌物三种气味源的选择频次进行了统计分析。结果表明:用三种气味源与对照作为刺激源时,中缅树鼩均显著选择尿液和胸腺分泌物(P0.05);在习惯性实验中中缅树鼩对同性尿液和胸腺分泌物的选择频次持续下降,对陌生个体尿液和胸腺分泌物的选择频次显著增加(P0.05);尿液、胸腺分泌物在雌雄中缅树鼩个体识别的影响表现出明显差异,中缅树鼩对尿液和胸腺分泌物的选择频次均显著多于对照组(P0.01),而且雄性胸腺分泌物较雌性更受关注(P0.05),但雌性尿液比雄性更受关注(P0.05)。以上所有结果说明,中缅树鼩的尿液和胸腺分泌物中包含个体信息并用以识别个体,这两种气味源中所包含的化学信号对中缅树鼩个体间的交流极为重要,而粪便可能仅为单纯的排泄生理。     

Effect of MTECl on the functional expression of TCRαβ~ + 3G11~- 6C10~-CD4~+CD8~- thymocyte subset

A murine CD4+ thymocyte subset with phenotype of TCRαβ + 3G11- 6C10- CD4 + CD8- CD69 +/- HSAmed/locontains the cells in relatively functional matured status. The functional property of the cells in this subset is characterized by the unique pattern of cytokine production at transitional stage from Th0 to Th2 type with the latter being the dominant type. After being co-cultured with murine thymic medullary epithelial cell line (MTEC1) cells, a murine thymic medullary type epithelial cell line, the TCRαβ(T 3G11 6C10-CD4 + CD8- CD69+/- HSAmed/l? thy-mocytes, has exhibited significantly higher levels of proliferation capability and IL-6 production, whereas the production of IL-4 and IL-10 is suppressed after co-culturing with MTECl. By contrast, MTECl could not induce thymocytes to secrete Th1 type of cytokines. The results suggest that MTECl can regulate functional status of this thymocyte subset and induce them to develop into a specialized Th2 subset.     

不同栽培基质对切花菊生长发育的影响

通过盆栽试验,研究7种不同基质对切花菊生长发育的影响,结果表明:棉籽壳 腐熟稻草的效果最佳,其次是锯木屑 腐熟稻草,蘑菇渣 腐熟稻草居三,其它4种基质均较差.     

Production of insulin-like growth factor I (IGF-I) and IGF-binding proteins by rat intestinal stromal cells in vitro

To determine if intestinal stromal cells secrete diffusible factors such as insulin-like growth factors (IGFs) capable of regulating epithelial cell growth in vitro, stromal cells were isolated by enzymatic digestion of rat intestine. Incorporation of [³H]thymidine into DNA and [¹⁴C]leucine into protein of IEC-6 cells, a model intestinal epithelial cell line, was significantly increased (two- to threefold) when the IEC-6 cells were co-cultured with stromal cells, relative to IEC-6 cells grown alone. Medium conditioned by stromal cells stimulated DNA synthesis of IEC-6 cells in a dose-dependent manner. Analysis of the conditioned medium revealed that intestinal stromal cells secreted IGF-I, but little IGF-II, in addition to an M _r 32,000 IGF-binding protein (IGFBP-2) and an IGFBP having M _r∼ 24,000. We conclude that rat intestinal stromal cells secrete one or more diffusible factors, which may include IGF-I and IGFBPs, capable of stimulating proliferation of IEC-6 cells in vitro. Received 25 August 1997; received after revision 7 November 1997; accepted 20 November 1997     

菠菜有机生态型无土栽培技术研究

以菇渣、锯木屑、珍珠岩作为有机生态型栽培基质,采用单纯形-格式设计方法,研究这3种基质对菠菜产量和品质的影响。结果表明,菇渣在提高产量及维生素C含量、降低硝酸盐含量上影响最大,锯木屑在降低可溶性草酸含量上效果最好。通过综合选优后,得到菠菜高产优质的适宜基质配比为菇渣∶锯木屑∶珍珠岩=0.27∶0.5∶0.23。     

猪血清胸腺因子免疫活性的测定

根据哺乳动物循环血液中所含有的血清胸腺因子(STF)能使θ-阴性的玫瑰花瓣状形成细胞(RFC~-)转变成RFC~+,同时提高其对抗θ抗原血清的代用品硫唑嘌呤的敏感性的原理,采用成熟的BALB/C品系小鼠,经摘去胸腺后的鼠脾脏淋巴细胞和绵羊红细胞(SRBC)之间,在STF和硫唑嘌呤同时共存的条件下具有抑制玫瑰花结形成的作用,从而建立了对哺乳动物循环血液中的STF(九肽)的超微量生物活性测定方法。用本方法对猪血清和血浆的检测结果,其活性滴度分别为1:512、1:1024。     

Effect of MTEC1 on the functional expression of TCRaβ+ 3G11− 6C10− CD4+ CD8− thymocyte subset

A murine CD4⁺ thymocyte subset with phenotype of TCRαβ⁺ 3G11⁻ 6C10⁻ CD4⁺ CD8⁻ CD69^+/- HSA^med/lo contains the cells in relatively functional matured status. The functional property of the cells in this subset is characterized by the unique pattern of cytokine production at transitional stage from Th0 to Th2 type with the latter being the dominant type. After being co-cultured with murine thymic medullary epithelial cell line (MTEC1) cells, a murine thymic medullary type epithelial cell line, the TCRαβ⁺ 3G11⁻ 6C10⁻ CD4⁺ CD8⁻ CD69^+/- HSA^med/lo thymocytes, has exhibited significantly higher levels of proliferation capability and IL-6 production, whereas the production of IL-4 and IL-10 is suppressed after co-culturing with MTECl. By contrast, MTECl could not induce thymocytes to secrete Thl type of cytokines. The results suggest that MTECl can regulate functional status of this thymocyte subset and induce them to develop into a specialized Th2 subset.     

胸腺内双阴胸腺细胞重建动态的数学模型

根据小鼠的胸腺细胞实验数据,建立了描述胸腺细胞（ｔｈｙｍｏｃｙｔｅ）发育过程的数学模型,该模型考虑了胸腺细胞与胸腺基质细胞（ｔｈｙｍｉｃ ｓｔｒｏｍａｌ ｃｅｌｌ,ＴＳＣ）之间的相互作用,能够模拟ＣＤ４－ＣＤ８－ＤＮ细胞重建动态及正常小鼠胸腺细胞的发育过程,研究表明,模型中的一些参数应该是含有时间延迟的,通过模型研究了胸腺细胞与ＴＳＣ之间亲和力在胸腺选择过程中的阳性与阴性选择中的差异,理论预言与实