猪附红细胞体病的实验室诊断

通过对奎屯某猪场病猪的临诊表现及剖解变化、猪附红细胞体病原的形态观察、体外培养试验、感染试验以及感染情况的调查,确定该病为附红细胞体病。     

A thiol protease of peritoneal macrophages in the guinea pig

Summary Proteolytic enzymes of the guinea pig peritoneal exudate macrophages were investigated using synthetic fluorogenic peptide substrates. Among several enzymes, t-butyloxycarbonyl-phenylalanyl-seryl-arginine 4-methylcoumaryl-7-amide cleaving enzymes had the highest activity, and the activity in exudate macrophages was about 3 times tronger than that in resident thiol-blocking reagents, suggesting a thiol protease.We thank Dr Yoshiaki Motozato, Kumamoto University, for kind donation of Cellulofine GC-700 and valuable discussions. This work is supported in part by the Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.     

Different intrafusal fiber composition of spindles in sheep and pig extraocular muscles

Summary Histochemical profiles of intrafusal fibers have been examined in muscle spindles of extraocular muscles of sheep and pig. Results show that in the sheep the intrafusal content presents, in addition to chain fibers, at least one bag₁ and one bag₂ fiber, whereas in the pig almost all the spindles are bag₁-fiber spindles.Acknowledgment. This study was supported by grants from C.N.R. and M.P.I.     

硫酸水解角蛋白制取L-胱氨酸的研究

研究了硫酸法水解角蛋白提取Ｌ－胱氨酸,讨论了由猪毛提取Ｌ－胱氨酸工艺的主要影响因素。结果表明,Ｌ－胱氨酸的水解率高达１３％～１４％,收率可达６．８％,母液制成氨基酸复合肥,总氨基酸含量大于２０％。     

幽门螺杆菌豚鼠动物模型的建立

采用哥伦比亚血琼脂固体培养基培养的幽门螺杆菌国际准菌株NCTC11637,用生理盐水稀释至109CFU/mL,采用6周龄、体重均在300~350 g的豚鼠20只,随机分为2组,每组对照鼠2只.实验鼠连续3次灌注幽门螺杆菌生理盐水稀释液,对照鼠同时灌注生理盐水,6、8周后处死检测.发现对照组豚鼠鼠胃中有Hp定值并呈现明显炎症反应,鼠胃上皮单个核细胞浸润,表明豚鼠对Hp NCTC11637敏感.首次在豚鼠中建立了稳定、可靠的幽门螺杆菌敏感动物模型,可为幽门螺杆菌疫菌的免疫学测定打下基础.     

Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension

Great progress have been made in animal cloning in China, as evidenced by the live births of cloned cat- tle[1,2], goats[3,4], and sheep[5]. In contrast, pig cloning is still in its infancy though limited fundamental studieshave been conducted[6]. It is g…     

颈内动脉注射尿激酶治疗急性脑梗死的疗效观察

应用颈内动脉注射尿激酶治疗30例急性脑梗死患者,并与对照组（静脉滴注尿激酶）30例比较。结果显示治疗组疗效明显优于对照组（P＜0．01）,且无1例发生颅内出血。提示颈内动脉注射尿激酶治疗急性脑梗死,疗效显著而安全。     

猪附红细胞体人工感染及药物治疗初步研究

以猪源附红细胞体进行了人工感染试验 ,结果初步表明 :猪附红体可使猪、羊感染发病 ,而鸡不感染。感染动物红细胞总数减少 ,血红蛋白含量降低 ,红细胞脆性增高 ,白细胞数增多。体内外药物敏感性试验表明 ,血虫净和盐酸土霉素配合使用治疗效果良好     

管道内腐蚀检测技术进展

介绍了国内外管道内腐蚀检测技术的发展状况 .针对国外先进的漏磁法智能爬行机和超声波智能爬行机 ,分析了其检测管道内腐蚀的原理 ,以及这类智能装置的基本组成和工作过程 .同时指出了国内管道腐蚀检测技术所存在的差距和亟待解决的问题     

贵州小型猪病原生物携带和感染状况的调查

目的检测贵州小型猪病原生物携带和感染状况,为进行微生物与寄生虫控制提供依据。方法采集贵州小型猪封闭群皮毛、分泌物、血液及粪便等标本,采用形态观察、生化反应和血清学方法检测不同部位共36种微生物与寄生虫指标。结果在36种检测指标中,该种群对28种病原生物有不同程度的携带和感染。结论贵州小型猪的病原携带和感染现状是对其进行微生物与寄生虫监测和控制的主要依据。