Hg2＋对青海湖裸鲤肾脏组织的细胞毒性研究

为了解重金属Hg2＋对青海湖裸鲤肾脏组织的细胞毒性;以含有Hg2＋的浓度为0.0025、0.005、0.01、0.02mg/L的培养液培养裸鲤肾脏组织细胞;结果表明,0.005mg/L的Hg2＋浓度为裸鲤肾脏细胞毒性死亡的临界浓度,且裸鲤肾脏细胞的死亡率随Hg2＋的浓度的增加而增大,当培养基的Hg2＋浓度达到0.02mg/L时细胞全部死亡.     

Role of dystrophin and utrophin for assembly and function of the dystrophin glycoprotein complex in non-muscle tissue

The dystrophin glycoprotein complex (DGC) is a multimeric protein assembly associated with either the X-linked cytoskeletal protein dystrophin or its autosomal homologue utrophin. In striated muscle cells, the DGC links the extracellular matrix to the actin cytoskeleton and mediates three major functions: structural stability of the plasma membrane, ion homeostasis, and transmembrane signaling. Mutations affecting the DGC underlie major forms of congenital muscle dystrophies. The DGC is prominent also in the central and peripheral nervous system and in tissues with a secretory function or which form barriers between functional compartments, such as the blood-brain barrier, choroid plexus, or kidney. A considerable molecular heterogeneity arises from cell-specific expression of its constituent proteins, notably short C-terminal isoforms of dystrophin. Experimentally, the generation of mice carrying targeted gene deletions affecting the DGC has clarified the interdependence of DGC proteins for assembly of the complex and revealed its importance for brain development and regulation of the ’milieu intérieur. Here, we focus on recent studies of the DGC in brain, blood-brain barrier and choroid plexus, retina, and kidney and discuss the role of dystrophin isoforms and utrophin for assembly of the complex in these tissues. Received 4 October 2005; received after revision 14 March 2006; accepted 5 April 2006     

B超发现三家族14例多囊肾临床报告

通过对三家族成员B超检查发现多囊肾14例,说明多囊肾与遗传有关,提出B超对高血压患者的病因具有鉴别意义,同时B超对有多囊肾家族史的成员进行普查是早期发现多囊肾及提供早期治疗的重要途径。     

The relationship among the reaction product colors of non-specific esterase by alpha-naphthyl acetate method

The present work is concerned with the relationship of the different colors of NSE (non-specific esterase) reaction product, in which alpha-naphthyl acetate and diazotate of o-dianisidine (Fast Blue B salt) were used as a substrate and a coupler respectively to describe the histochemical characteristics of NSE in chick embryonic mesonephros and metanephros. Our preliminary results throw light on the nature of NSE reaction produced colors, under light microscope, which were different in various sections for different airdrying time-length. The reaction product on the slices of the convoluted tubules of chick embryonic kidney was bright yellow after less than 30 min air-drying time-length, brown after 1.5≈2 h air-drying, time-length and black after more than 3 h of air-drying. The color-change process of NSE precipitates from bright yellow to black is progressive. The phenomennon has not been described previously. Supported by National Natural Science Foundation of China and Morning Sunlight Programe of Wuhan City Government Xu Xuehong: born in Oct. 1962, Professor     

Different patterns of agonist-induced modulation of α1B-adrenoceptor density at different initial expression levels

We compared the norepinephrine (NE) induced α1B-adrenoceptor (α1B-AR) expression modulation between two transfected human embryonic kidney (HEK) 293 cell lines in which α1B-AR densities were (6 336±913) and (773±164) fmol.mg-1, respectively. Treatment of cells with NE (10 μmol.L-1) for 48 h decreased high-level expressed α1B-AR density, but increased low-level expressed α1B-AR density. The protein kinase C inhibitor Calphostin C or Ro-31-8220 reversed, and its activator PMA mimicked the NE-induced down-regulation of high-level expressed α1B-AR. Moreover, PMA induced a down-regulation of low-level expressed α1B-AR. The endoplasmic reticulum Ca2+-ATPase inhibitor cyclopiazonic acid (CPA) and the calcium chelator BAPTA/AM did not affect the down-regulation of high-level expressed α1B-AR, but inhibited the up-regulation of low-level expression α1B-AR induced by NE. These results suggest that α1B-adrenoceptor densities at different initial expression levels are differentially regulated by NE and their signal transduction pathways are different.     

Effect of Nano Red Elemental Selenium on GPx Activity of Broiler Chick Kidney Cells in vitro

0　IntroductionSelenium(Se)isanessentialtraceele mentwithaverynarrowmarginbe tweennutrientlevelsofintakeandtoxicity .Anti oxidationisthemostimportanteffectofSe’sbiologicalfunction .Seisanessentialcomponentandactivesiteofcellularglu tathioneperoxidase (GPx) [1 ] .GPxistheoneofmainanti oxidizingenzymes,clearingdeleteriousperoxides,suchasH2 O2 ,fattyacidperoxide,phospholipidhydroperoxide,andsoon[2 4] ,toprotectbiologicalmem braneandbiologicalbigmoleculefromoxida tivedamageintissues[5 \〗.It…     

多克隆抗体治疗移植肾难治性排斥反应

为了解多克隆抗体（ＡＴＧ）治疗移植肾难治性排斥反应的结果，对２９例移植肾难治性排斥反应应用ＡＴＧ治疗。结果２４例急性排斥反应逆转，逆转率为８２７％。说明ＡＴＧ治疗难治性排斥反应效果显著，巨细胞病毒感染及过敏反应是ＡＴＧ治疗的主要副作用。     

应用离子交换纤维素提取外源凝集素的研究

应用ＤＥＡＥ－纤维素从花芸豆和白芸豆中提取外源凝集素，在平衡ＰＨ６．８的条件下，用酸度梯度洗脱的方法，得到了较好的柱色谱分离效果，用获得的外源凝集素组分进行淋巴细胞的激活培养试验，其效果十分显著。     

Relationship between DNA and cholesterol synthesis in kidney after refeeding

Summary DNA and cholesterol synthesis were investigated in the kidneys of fasted-refed rats. Refeeding resulted in an increase in kidney DNA synthesis, as measured by³H-thymidine incorporation, starting at 72 h. The increase in DNA synthesis was accompanied by a stimulation of cholesterol synthesis, as measured by¹⁴C-acetate incorporation into cholesterol.     

Influence of nocloprost on the vascular responses to various stimuli in the isolated perfused rabbit kidney

Summary Nocloprost, a new synthetic prostanoid, elicited a vasodilator action when given through the renal artery of the isolated perfused rabbit kidney. Lower concentrations of the compound inhibited the pressor response to sympathetic stimulation or to angiotensin II, but potentiated the pressor effect of exogenous noradrenaline. These results were taken as an evidence that nocloprost prevents the release of neurotransmitter by a presynaptic mechanism.This work was supported by a grant from the Turkish Scientific and Technical Research Council (TAG-578). Nocloprost is the gift of Dr E. Schillinger, Head of Biochemical Pharmacology, Schering AG Research Laboratories, Berlin, FRG.