Evidence for a role of IFN γ in control ofListeria monocytogenes in T cell deficient mice

Summary The role of interferon (IFN) in controlling chronic infections ofListeria monocytogenes (Listeria) was studied in athymic C57BL/6nu/nu mice, and by treating thymectomized C57BL/6 +/+ mice with monoclonal rat CD4 and CD8-specific monoclonal antibodies (Mab). Mice treated with a combination of the two T cell subset antibodies were similar to athymic, nude mice in being able to contol Listeria infection, keeping the titers below 3–5 log₁₀ bacteria per organ, but they could not eliminate them completely. Treatment with antibodies to IFN of nude or CD4⁺ + CD8⁺-T cell-depleted mice suffering from chronic Listeria infection caused a marked increase of Listeria titers, in liver and spleen. This result implies a role of IFN in maintaining anti-Listeria resistance in mice lacking mature T cells.     

我国基因工程干扰素的研究概况

介绍了基因工程干扰素及其应用，以及影响基因干扰素产业化生产的因素，并对我国基因工程干扰素的发展历程、现状和前景作了阐述。     

αA干扰素基因在酿酒酵母中的表达

将含有αA干扰素基因的DNA片段接上化学合成的EcoR I-Hind III转换接头,然后插入酵母分泌型表达载体YFD 18中,构建成质粒YFD 66和YFD66-0.转化酵母菌BJl991后,得到转化子BJ1991/YFD66和BJl991/YFD66-0.干扰素活性测定表明,每升BJ1991/YFD66培养液含有αA干扰素10~6U,而BJ1991/YFD66-0则不产生干扰素,YFD66为αA干扰素表达质粒。