福建水生药用植物资源的研究

通过对福建水生药用植物资源的调查,初步摸清了福建水生药用植物的种类、地理分布及其与生态环境的关系。在此基础上,分析了福建水生药用植物的区系成分和生活型,提出福建水生药用植物资源的合理利用与保护的意见,为进一步研究福建水生药用植物资源提供基础资料和有关的科学依据。     

中国产植物新种

本描述了中国产植物１９个种及２个新变种。新种是：粗炷银莲花、峨眉楠、纤轴楠、坝王新木姜子、阿沛悬钩子、米兰山小桔、隐脉沟瓣花、四蕊八角枫、伞花锦香草、黑叶鹅掌些、腺紫金牛、毛果柿；石生柿、张氏柿、榄果柿、高盘萝芙木、窝穴素馨、裂叶紫珠、拟珠；新变种是：光果威灵仙、少花卵叶锦香草。全部模式标本存中山大学植物标本室。     

Pathogenic enterococci: new developments in the 21st century

Enterococci, traditionally viewed as Gram-positive commensal bacteria inhabiting the alimentary canals of humans and animals, are now acknowledged to be organisms capable of causing life-threatening infections in humans, especially in the nosocomial environment. The existence of enterococci in such a dual role is facilitated, at least in part, by its intrinsic and acquired resistance to virtually all antibiotics currently in use. Beginning with the initial identification of a streptococci of fecal origin in the late 19th century, enterococci have been studied for over a century now. A number of comprehensive reviews during this time have addressed various aspects of enterococci, including classification, biology, virulence, antibiotic resistance and so on. This review specifically addresses the important advances in the field of enterococcal research that have occurred since the beginning of the 21st century. Most notable among these developments have been the insights into enterococcal genomes and pathogenicity.Received 10 April 2003; received after revision 31 May 2003; accepted 3 June 2003     

西藏米拉山区种子植物区系研究

西藏米拉山区有种子植物71科304属728种,其中裸子植物有3科7属12种。该区植物地理成分复杂,特有现象明显,从科、属和种的分布类型统计分析,均表现为温带成分最多,分别占本层次的比例为43．66％,84．62％和58．72％,反映出典型的温带区系特征和区系的年轻性。依据种的丰富程度和一些自然地理条件的特征将米拉山区系划分为2个植物区系小区：米拉山东坡小区、米拉山西坡小区。与此相毗邻的色季拉山和墨脱比较,米拉山区系与色季拉山的区系性质更为相似。     

甘肃白水江国家级自然保护区植物区系属的整体分析

甘肃白水江国家级自然保护区地理位置独特,植物种类丰富,种子植物共计183科、718属、1970种.分析了区内各属的数量、分布型、生活型等几个方面,结果表明：本区系是一个温带区系,但杂有一定程度的热带-亚热带性质;地理成分复杂,体现其与世界及国内各植物区系的广泛联系;原始与年轻类群大量并存,表明该区既是古老类群的保存中心,又是新类群分化发生的活跃区域;从中国特有属的分布式样等证据来看,本区与华中区系的关系更为密切.     

武夷冻茶霉菌菌相分析及优势菌ITSrDNA鉴定

为研究武夷冻茶霉菌的分布情况,采用镜相分析和PCR（polymerase chain reaction）技术对霉菌进行菌相分析及优势菌种鉴定。结果表明,冻茶中主要菌属有曲霉属、毛霉属、根霉属和青霉属,其中青霉属为优势菌属;对随机分离纯化的青霉进行ITSrDNA鉴定,鉴定结果为壳青霉（Penicillium.crustosum Thom）。分析结果可为微生物污染途径分析以及改进冻茶的冷藏工艺条件提供理论依据。     

Uninterrupted protein synthesis is essential for survival in the early stages of carbontetrachloride-induced hepatocellular necrosis in the mouse

Summary The fatal syndrome produced by cycloheximide given 6 h after a hepatonecrogenic dose of CCl₄ is due neither to direct toxic synergism between CCl₄ and cycloheximide nor to transient sinusoidal thrombosis. It is suggested that survival in the presence of unknown factors released from dying liver cells requires uninterrupted protein synthesis. The life-saving effect of sterilization of the intestine by antibiotics indicates that the gut flora or its products play a vital role in pathogenesis.Acknowledgments. I wish to thank Mr C. R. West for carrying out the statistical analysis, Mrs Brenda Brooks for histological processing, and Berk Pharmaceuticals Ltd for information on Ancrod defibrination in mice.     

The enteric neural receptor for 5-hydroxytryptamine

Summary An enteric neural receptor for serotonin (5-HT) has been characterized. This receptor was assayed, using³H-5-HT as a radiologand, by rapid filtration of isolated enteric membranes and by radioautography. In addition, intracellular recordings were made from ganglion cells of the myenteric plexus. High affinity, saturable, reversible, and specific binding of³H-5-HT was demonstrated both to membranes of the dissected longitudinal muscle with adherent myenteric plexus and the mucosa-submucosa. Radioautographs showed these³H-5-HT binding sites to be in myenteric ganglia and in a broad unresolved band at the mucosal-submucosal interface. Antagonists active at receptors for other neurotransmitters than 5-HT, at either of the two known types of CNS 5-HT receptor, and at 5-HT uptake sites on serotonergic neurons failed to inhibit binding of³H-5-HT. The structural requirements of analogues for binding to the enteric 5-HT receptor matched the known pharmacology of M or neural 5-HT receptors. A novel 5-HT antagonist was found. This compound, N-acetyl-5-hydroxytryptophyl-5-hydroxytryptophan amide (5-HTP-DP), antagonized the action of 5-HT on type II/AH cells of the myenteric plexus but did not affect the release or actions of acetylcholine (nicotinic or muscarinic) or substance P. 5-HTP-DP was also an equally potent displacer of³H-5-HT from its binding sites on enteric membranes. It is concluded that the sites responsible for specific binding of³H-5-HT are enteric M or neural 5-HT receptors. These receptors differ from those now known to be present in the CNS.     

不同摄入量小米对小鼠肠道菌群和短链脂肪酸的影响

摄入一定量杂粮可降低一些慢性代谢疾病的发病率,但目前杂粮摄入量没有统一的标准,尚不清楚杂粮摄入过多是否会对健康产生不良影响。以小米添加量为20%、40%、60%、80%的饲料喂养3周龄C57BL/6J小鼠,持续12周,采用自动血生化分析仪、16S rRNA高通量基因测序、气相色谱-质谱联用仪分析了不同摄入量小米对小鼠血脂水平、肠道菌群和粪便短链脂肪酸的影响。结果发现,80%摄入量的小米显著增加了小鼠血清的总胆固醇、高密度脂蛋白胆固醇和低密度脂蛋白胆固醇的水平,同时增加了肠道丙酸、丁酸、异丁酸和戊酸的含量。肠道菌群分析结果表明,所有小米干预组的拟杆菌门(Bacteroidota)、Muribaculaceae的丰度上升,厚壁菌门(Firmicutes)、放线菌门(Actinobacteriota)、乳杆菌科(Lactobacillaceae)、双歧杆菌属 (Bifidobacterium)的丰度下降。摄入不同添加量小米的小鼠肠道菌群组成具有较大差异,其中20%小米摄入量组的小鼠菌群中显著富集了另枝菌属(Alistipes)、副拟杆菌属(Parabacteroides)、肠杆菌属(Enterorhabdus),而80%摄入量小米显著降低了小鼠菌群中的粪杆菌属(Faecalibaculum)、布劳特氏菌属(Blautia)和罗氏菌属(Roseburia)的丰度。研究结果表明,20%摄入量的小米就能有效调节小鼠肠道菌群,而过高摄入量(80%)的小米使小鼠血脂水平升高,降低了肠道菌群的多样性和均匀度以及有益菌的丰度,所以要理性看待杂粮的营养价值,避免过量摄入。