Protective effect of Shigyaku-to,a traditional Chinese herbal medicine,on the infection of herpes simplex virus type 1 (HSV-1) in mice

The antiviral activity of Shigyaku-to (TJS-109), a traditional Chinese herbal medicine, was investigated in mice infected with herpes simplex virus type 1 (HSV-1). TJS-109 is a combination of the medicinal plant extracts fromZingiberis siccatum rhizoma,Aconiti tuber andGlycyrrhizae radix in a specific proportion. Mice infected with a 10 LD₅₀ dose of HSV-1 were treated with TJS-109 orally at doses of 1.25 to 20 mg/kg 2 days before, and 1 and 4 days after the infection. The treated groups had 80% (1.25 mg/kg), 40% (5 mg/kg) and 23% (20 mg/kg) mortality rates 25 days after the infection as compared with a 100% mortality rate in control mice treated with saline. When HSV-1 infected mice (recipients) received CD8⁺T cell fractions derived from spleens of mice treated with TJS-109 (donors), 70% of recipients survived, as compared with 0% survivors in the groups of mice treated with saline, B cell fractions, CD4⁺ T cell fractions or macrophage-enriched fractions prepared from the same donors. TJS-109 did not show any virucidal activities against HSV-1 or any virostatic activities on the growth of HSV-1 in Vero cells. These results suggest that TJS-109 protected mice exposed to lethal amounts of HSV-1 through the activation of CD8⁺ T cells.     

A morphometric study of spermatogenesis in the testes of mice of a senescence accelerated strain

The morphometric parameters of spermatogenic cells in a mouse strain prone to accelerated senescence (SAM-P), a novel murine model of spontaneously promoted aging, were compared with those of a SAM resistant strain (SAM-R) after birth until 40 weeks (mean life span of SAM-P). A mixture of gonocytes and spermatogonia were present in the testis in 1-week-old mice, and no gonocytes were observed in 2-week-old mice. At 6 weeks of age, the absolute number of spermatogonia in SAM-P was 27% greater than that in SAM-R, whereas the cell number in 40-week-old SAM-P was 17% less than in SAM-R. Primary spermatocytes were first observed in 3-week-old animals, and the cell numbers in SAM-P at 3, 5 and 6 weeks were 78%, 31% and 25%, respectively, greater than in SAM-R, whereas the cell number in SAM-P at 40 weeks was 30% less than SAM-R. Round spermatids were first observed in all SAM-P at 4 weeks old, but 20% of SAM-r had no spermatids and the rest had only a few. At 5 and 6 weeks old, the absolute numbers of round spermatids in SAM-P was about 34% and 41%, respectively, greater than in SAM-R, whereas the cell number in 40-week-old SAM-P was about 34% less than SAM-R. These results indicate that testicular maturation begins at an earlier age in SAM-P than SAM-R. Furthermore, at the age of 40 weeks signs of testicular deterioration are evident in SAM-P mice only     

n^＋／p常规硅太阳电池表面“死层”的减少方法

磷透过热生长氧化层扩散的方法，可以减缓高浓度浅结磷扩大硅表层带来的晶格损伤（即“死层”）双晶Ｘ射线衍射图形和杂质纵向分布测量均证实了这一点，用此方法制备的常规结深的太阳电池，其相对光谱响应４００～６００ｎｍ波段内平均提高了５０％，在ＡＭＯ（２５℃）条件下，其光电转换效率也从常规电池的１１．８％提高到１２．３％，转换效率可望在工艺最佳化研究后得到进一步提高。     

用细胞神经网络实现蔡氏混沌电路

研究了用细胞神经网络（ＣＮＮ）实现蔡氏电路的方法，把ＣＮＮ模型与蔡氏电路模型两者联系起来，并且只采用一般常用的运算放大器、电阻和电容即可方便地构成蔡氏电路，试验结果表明，电路原理简明，工作可靠，性能优良，有较大的实用价值。     

硬化性肌膜炎患者血清ACA的发现及对小鼠细胞着丝点的研究

首次在硬化性肌膜炎患者血清中发现有抗着丝点抗体(ACA),可使 Hep-2间期细胞核及 M 期细胞染色体着丝点显示分散的荧光斑点.用 ACA 血清对小鼠几种器官的冰冻切片或涂片进行免疫荧光研究,可见无论幼龄或老龄小鼠的肝、十二指肠、脑皮层细胞及老龄小鼠睾丸生精细胞均有明亮的荧光斑点.绒毛顶端细胞呈均匀性荧光.     

玉米根细胞正面向外和内翻外质膜囊泡的分离

利用水双相分配分离法从玉米根细胞提取原生质膜囊泡。其质膜成分特异的K~+,Mg~(2+)—ATP酶活性比膜粗提液高1.67倍。其他细胞器标记酶活性在质膜组分中很低或没有。特异染色电镜形态学定量统计表明质膜组分中90％以上是质膜囊泡。去污剂刺激ATP酶活性实验证明,约92％囊泡是正面向外的。应用此法也证明NAD(P)H—氧化酶可能是跨膜存在的,其催化Fe~(3+)还原的位点可能在膜的内侧。应用高盐冻—融法并结合水双相分配分离法处理正面向外囊泡,可获得内翻外质膜囊泡,实验证明它不仅纯度高而且封闭。     

利用固定化酵母细胞生产ATP

利用明胶戊二醛制备的固定化啤酒酵母细胞,由腺苷酸(AMP)生产腺嘌呤核苷三磷酸(ATP)。在含有15 mmol/L AMP,75 mmol/L葡萄糖,5mmol/L硫酸镁(MgSO_4·7 H_2O)的0.2 mol/L磷酸缓冲液(pH 7.0)中,在34℃振荡反应1h,ATP转化率可达100%,分批连续反应10次,ATP转化率保持在90%以上。     

化学刺激引起相邻丽藻细胞的重复兴奋发放

给一丽藻细胞以化学刺激,经过一段延时,发现与之相邻的细胞的膜电位出现了自持重复兴奋发放.这一现象,可能是阳离子通过细胞节的扩散而引起的.