Effects of Genistein on Cell Cycle and Apoptosis of Two Murine Melanoma Cell Lines

The effects of genistein on several tumor cell lines were investigated to study the effects of gen- istein on cell growth, cell cycle, and apoptosis of two murine melanoma cell lines, B16 and K1735M2. These two closely related murine melanoma cell lines, however, have different responses to the genistein treat- ment. Genistein inhibits the growth of both the B16 and K1735M2 cell lines and arrests the growth at the G2/M phase. After treatment with 60 μmol/L genistein for 72 h, apoptosis and caspase activities were de- tected in B16 cells, while such effects were not found in K1735M2. Further tests showed that after genistein treatment the protein content and mRNA levels of p53 increased in B16, but remained the same in K1735M2. The protein content and mRNA levels of p21WAF1/CIP1 increased in both cell lines after treatment. The results show that genistein might induce apoptosis in B16 cells by damaging the DNA, inhibiting topoi- somerase II, increasing p53 expression, releasing cytochrome c from the mitochondria, and activating the caspases which will lead to apoptosis.     

The inner ear structures of the echidna—An SEM study

Summary Surface structures of the echidna cochlea were investigated using a scanning electron microscope technique. It was found that unlike typical mammalian cochleas, the echidna cochlea possesses four rows of inner hair cells and 6–9 rows of outer hair cells, and that the arrangements of the stereocilia of the outer hair cells were not uniform throughout the length of the basilar membrane.We are grateful for the valuable advice given by Mr J. Nailon.     

Cu2ZnSnS4/Zn(O,S)异质结薄膜太阳电池的数值仿真研究

Zn(O,S)具有带隙宽、原料丰富且环保的优点,是一种很有潜力的CdS替代缓冲层。本文采用Solar Cell Capacitance Simulator (SCAPS)软件,对CZTS/Zn(O,S)/Al:ZnO 结构的薄膜太阳电池进行数值仿真,主要模拟研究Zn(O,S)的禁带宽度和电子亲和势、缓冲层的厚度及掺杂浓度、环境温度对电池性能的影响。结果表明：当Zn(O,S)的厚度和载流子浓度分别为50 nm和10₁₇ cm_-3时,电池的转换效率可达14.90％,温度系数为-0.021%/K。仿真结果为Zn(O,S)缓冲层用于CZTS太阳能电池提供了一定的指导。     

Pain relief by xenograft of subarachnoid microencapsulated bovine chromaffin cells in cancer patients

The bovine chromaffin cells (BCC) implanted into the subarachnoid space can release analgesic substances such as opioid peptides and ealeeholamines. Clinical trials have provided the evidence that the implantation of polyvinylchloride ( PVC) hollow fiber encapsulated BCC by surgery can relief the pain in cancer patients. In the present study, BCC were encapsulated in alginate-polylysine-alginate (APA) mieroencapsules which protect the grafting of xenogeneic cells from host immune system anil allow BCC to function effectively without using immunosuppression agents. The microencapsulated BCCs (5 X 106~—8 X 106) were transplanted into the subarachnoid space I^._s of 17 patients who suffered from chronic cancer pain and had to have long-term administration of analgesics. The pain scores and morphine intake tesl showed that microencapsulated BCC graft totally stopped the chronic pain in three of the patients over a period of 200 days and in the other three over a period of 100 days. The resulls suggesl thai APA microencapsulated BCC xenotransplantation could be a novel alternative approach to managing pain of cancer patients.     

乙,丙型病毒性肝炎血清影响淋巴细胞集落形成的研究

病毒对造血有一定的抑制作用，用乙．丙烯肝炎病毒血清加入培养的正常细胞的上清中，研究其对淋巴细胞集落形成的作用，结果提示：乙、丙型病毒性肝炎血清，对有分裂原ＣｏｎＡ和ＰＷＭ激活的辅助Ｔ淋巴细胞和非Ｔ淋巴细胞，均有明显的抑制作用，对植物凝集素ＰＨＡ激活的总Ｔ淋巴细胞有一定的抑制作用，但不显著。乙－丙型肝炎病毒可能是引起某些造血细胞抑制的原因之一。     

抗坏血酸和亚硒酸钠对肝癌细胞逆转的研究

采用细胞倍增时间、分裂指数、生化变化和软琼脂克隆形成率等指标测定分化,研究了抗坏血酸和亚硒酸钠对人肝癌细胞逆转的作用.结果表明用抗坏血酸4mmol*L-1联合亚硒酸钠2μmol*L-1处理细胞后,细胞的生长率和有丝分裂指数都显著下降.一些与细胞恶性表型有关的指标,如细胞表面电荷明显下降,电泳率从1.74μm*s-1*V-1*cm-1下降到0.91μm*s-1*V-1*un－1,甲胎蛋白含量从331μg*g-1(蛋白)下降到90μg*g-1,γ-谷氨酰转肽酶活性从0.74U*g-1(蛋白)下降到0.19U*g-1.与细胞分化有关的指标,如酪氨酸-α-酮戊二酸转氨酶（TAT）的活性从14.7μmol*g-1(蛋白)升高至42.6μmol*g-1,软琼脂克隆形成能力下降94.7%.由此可见,抗坏血酸和亚硒酸钠联合能诱导肝癌细胞在分化,逆转肿瘤细胞的恶性表型.     

Effect of MTEC1 on the functional expression of TCRaβ+ 3G11− 6C10− CD4+ CD8− thymocyte subset

A murine CD4⁺ thymocyte subset with phenotype of TCRαβ⁺ 3G11⁻ 6C10⁻ CD4⁺ CD8⁻ CD69^+/- HSA^med/lo contains the cells in relatively functional matured status. The functional property of the cells in this subset is characterized by the unique pattern of cytokine production at transitional stage from Th0 to Th2 type with the latter being the dominant type. After being co-cultured with murine thymic medullary epithelial cell line (MTEC1) cells, a murine thymic medullary type epithelial cell line, the TCRαβ⁺ 3G11⁻ 6C10⁻ CD4⁺ CD8⁻ CD69^+/- HSA^med/lo thymocytes, has exhibited significantly higher levels of proliferation capability and IL-6 production, whereas the production of IL-4 and IL-10 is suppressed after co-culturing with MTECl. By contrast, MTECl could not induce thymocytes to secrete Thl type of cytokines. The results suggest that MTECl can regulate functional status of this thymocyte subset and induce them to develop into a specialized Th2 subset.     

日照强度和内部电阻对太阳能电池光伏性能的影响

在分析太阳能电池等效直流电路的基础上,利用电流-电压特性的函数关系式,建立了太阳能电池的仿真模型,研究了日照强度和内部电阻对电池伏安特性和光伏性能（如短路电流、开路电压、填充因子、输出功率和光电转换效率）的影响.研究结果表明：日照强度和内部串联电阻不仅明显影响了太阳能电池的伏安特性、短路电流和开路电压,而且也明显影响了太阳能电池的输出功率、填充因子和光电转换效率,同时太阳能电池的输出特性具有明显的非线性特征,并且存在一个最大的输出功率点和一个最佳的负载电阻值.     

Binding and endocytosis of monoterbium transferrin by K562 cells

Human serum apotransferrin is the major iron-tran- sport protein in blood. The protein binds iron as Fe3+ at two similar, but not identical, high-affinity binding sites. Although iron bound to human serum apotransferrin is less then 0.1% (~3 mg) of the total body iron, dynamically it is the most important iron pool with the highest rate of turnover. The turnover of transferrin iron is in the order of 30 mg per day and, normally, about 80% of this iron are transported to the bone marrow for hem…     

Mouse neural stem cells culturedin vitro and expressing an exogenous gene

Neural stem cells are the multipotential, self-renewing cells in central nerve system, and play an essential role in the development and differentiation of nerve system. Neural stem cells can be used to treat the nerve system diseases, especially, the transplantation of neural stem cells to rescue the degenerated neural cells has become a very promising therapeutic way. We successfully cultured neural stem cells isolated from the brains of embryonic micein vitro and determined their distribution in the E17 mice brains. The neural stem cells were transfected with adenoviral vector carrying GFP (green fluorescence protein) gene and then highly expressed the exogenous gene. It paves the way for gene therapy of degenerative nerve system diseases.