免疫系统对家畜生殖功能的调节和影响（综述）

免疫系统及其由免疫细胞产生的生物活性蛋白或类脂与大多数生殖细胞的产生复杂的网络交互作用，从而调节神经内分泌和生殖功能。本文从免疫系统与生殖道，输精的免疫反应，细胞因子的作用及免疫系统与生殖腺的功能，与妊娠，泌乳以及公母畜不孕方面综述了近年的研究文献。     

白介素-10对TNF-α介导血管平滑肌细胞增殖的影响

观察重组人白介素 10 (rhIL_10 )对肿瘤坏死因子 (TNF_α)刺激的离体大鼠胸主动脉血管平滑肌细胞增殖的影响。体外培养SD大鼠胸主动脉血管平滑肌细胞 ,采用MTS PES法确定血管平滑肌细胞的增殖状态。结果显示 ,TNF_α对血管平滑肌细胞增殖具有明显的刺激作用。rhIL_10单独应用对血管平滑肌细胞生长没有影响。在TNF_α刺激下 ,低至 10ng mL的rhIL_10可明显抑制血管平滑肌细胞的生长 (P <0 0 5 )。     

早期胚胎体外培养相关细胞因子研究进展

综述了五种细胞因子(表皮生长因子,转化生长因子,白血病抑制因子,胰岛素样生长因子和血管内皮生长因子)对于克服哺乳动物早期胚胎发育阻滞发挥的积极作用.     

Receptor role of membrane bound macrophage colony-stimulating factor

According to the definition of cytokine, the direction of signaling should be from cytokine to receptor. The counter receptor was presented. Membrane bound macrophage colony-stimulating factor (m-M-CSF) and its receptor (M-CSF-R) were shown in human leukemic cell line J6-1 as autojuxtacrine mechanism. Soluble M-CSF receptor (sM-CSF-R), which was isolated from J6-1 cells membrane, was added into J6-1 cell culture. It was observed inhibition of J6-1 cell proliferation, decreasing of mitosis index and ratio of multinuclear cells, enlargement of cell diameter and volume, down regulation of numerous surface antigens. Dramatic change of intracellular pH was shown between several min to 20 min after treatment of sM-CSF-R. It suggested that some information was transmitted via m-M-CSF from sM-CSF-R. This counter signaling was not influenced by saccharification of m-MCSF.     

考夫克和卡贝治疗分泌性中耳炎的机制研究

摘要: 目的研究抗过敏药物考夫克和卡贝治疗分泌性中耳炎的机制。方法采用卵蛋白致敏大鼠变态反应分泌性中耳炎动物模型,使用考夫克和卡贝分别予以治疗,在用药第7 天和第14 天采血及取PBS 中耳冲洗液,检测两者中干扰素γ( IFN-γ) 、白细胞介素4( IL-4) 、白三烯C-4( LTC-4) 、前列腺素D2( PGD2) 和血小板活化因子( PAF) 含量。结果用药第7 天,血清中及中耳冲洗液中上述细胞因子均较生理盐水治疗组偏低( P ＜ 0. 05) ; 与对照组比较,部分项目差异不显著( P ＞ 0. 05) ,但仍有个别项目有显著性差异( P ＜ 0. 05) 。用药第14 天,血清中及中耳冲洗液中上述细胞因子含量均恢复到正常水平,与对照组比较差异不显著( P ＞ 0. 05) 。结论考夫克或者卡贝的作用机制是通过抑制I 型变态反应,达到有效治疗分泌性中耳炎( OME) 的作用。     

Splenic B-cell activation in lipopolysaccharide-non-responsive C3H/HeJ mice by lipopolysaccharide ofPorphyromonas gingivalis

Porphyromonas gingivalis 381 lipopolysaccharide (LPS) definitely exhibited mitogenic activity in purified B-cells, separated from spleens of LPS-responsive C3H/HeN mice and LPS-non-responsive C3H/HeJ mice by using a magnetic cell sorting system. The mitogenic activity induced byP. gingivalis LPS was incompletely inhibited by polymyxin B.P. gingivalis LPS also induced a higher production of interleukin-6 (IL-6) in splenic B-cells of C3H/HeN mice as compared withEscherichia coli LPS. Furthermore,P. gingivalis LPS, but notE. coli LPS, induced definite IL-6 production in C3H/HeJ mice.P. gingivalis LPS increased tyrosine, serine/threonine phosphorylation of proteins with various major induced bands in splenic B-cells of both C3H/HeN and C3H/HeJ mice. Additionally, radioiodinatedP. gingivalis LPS, similarly toE. coli LPS, bound to a 73-kDa protein on C3H/HeJ as well as C3H/HeN B-cells. ThusP. gingivalis LPS may activate B-cells of C3H/HeJ as well as C3H/HeN mice via the LPS-specific binding protein on the cells.     

慢性乙型肝炎患者血清细胞因子检测的临床意义

目的:探讨慢性乙型肝炎患者血清转化因子(αTGF-)α和白细胞介素-8(IL-8)水平的变化。方法:应用放免法对44例慢性乙型肝炎患者进行了血清TGF-α和IL-8水平检测,并与34名正常人作比较。结果:慢性乙型肝炎组患者血清TGF-α和IL-8水平均显著地高于正常人组(P<0.01)。结论:检测慢性乙型肝炎患者血清TGF-α和IL-8水平对疾病的预后估计具有重要临床意义。     

一种高倍扩增细胞因子诱导杀伤细胞方法

CD3单克隆抗体分别以包被和悬浮的方式刺激人外周血单个核细胞,并以不同的基本培养基(RPMI-1640、IMDM、DMEM/F-12 (1:1)和M199)以及抗氧化剂(2-巯基乙醇和亚硒酸钠)培养细胞因子诱导杀伤(CIK)细胞,分别测定细胞的扩增、表型(CD3CD56和CD25)和非MHC-限制性毒性(NK 毒性和LAK毒性).结果显示高浓度CD3单克隆抗体包被刺激方式的细胞集落和细胞扩增倍数大于悬浮刺激方式.基本培养基DMEM/F-12 (1:1)的扩增倍数优于其他3种基本培养基.抗氧化剂2-巯基乙醇和亚硒酸钠均有促进CD25抗原形成的作用,亚硒酸钠能提高CIK细胞的毒性,而2-巯基乙醇对于细胞的扩增有促进作用.建立了一种高倍扩增CIK细胞的方法,20 d细胞平均扩增倍数可以达到千倍以上.     

117β-雌二醇对小鼠脾脏CD11c阳性树突状细胞白细胞介素和TNF-α表达的影响

在mRNA水平上研究17β-雌二醇(17β-estradiol,E2)对小鼠脾脏CD11c阳性树突状细胞(spleenCD11c-positive dendritic cells,SDCs)合成的细胞因子IL-6,IL-10,IL-12和TNF-α的调节作用.方法:免疫磁珠法纯化小鼠CD11c阳性SDCs,用不同浓度的...