选择性氧化酶基因及其表达的研究进展

综述了选择性氧化酶基因及其表达的研究进展，内容包括一些植物中的Aox多基因家族，以及植物的不同发育阶段、细胞色素途径和ROS对这些基因表达的影响。     

Successful identification of the final instar nymph of Quintilia carinata (Thunberg) (Hemiptera: Cicadidae) by DNA extraction from the exuvium

Despite being taxonomically and phylogenetically informative, the morphology of the immature stages of cicadas has received comparatively superficial attention. One reason for this is the difficulty of positively identifying immature stages, particularly as these stages are fossorial. We present a method for identifying cicada exuviae using DNA sequence data and describe a set of characters and character states for the final instar nymph of Quintilia carinata (Thunberg). The identification of immature stages using molecular methods will increase our knowledge of African cicadas, allowing for the initiation of future phylogenetic and ecological comparisons.     

Molecular phylogenetic status of Siberian roe deer （Capreolus pygargus） based on mitochondrial cytochrome b from Jeju Island in Korea

In order to elucidate the genetic diversity and taxonomic status of Siberian roe deer （Capreolus pygar- gus） from the Korean peninsula, partial sequences of the mitochondrial cytochrome b gene were analyzed. We col- lected 57 roe deer samples from Mainland and Jeju Island between July 2004 and June 2009. A total of 50 sequences （1,070 bp） were obtained, and seven haplotypes were defined. Five haplotypes were found from Mainland, and two were discovered from Jeju Island. The genetic diver- sity was lower in the Jeju Island population than in the Mainland population. In addition, both the consensus neighbor-joining tree and maximum parsimony tree showed an identical topology between the Mainland and Jeju Island clades. Based on the results, two subspecies of the Korean roe deer were confirmed. Siberian roe deer in Jeju Island showed a distinct difference in the cytochrome b gene compared with other Siberian roe deer, being aunique native species inhabiting only Jeju Island in Korea. Because Siberian roe deer in Jeju Island can be classified at subspecies level, the new subspecies in Jeju was named Capreolus pygargus jejuensis in this study. For this new subspecies, reasonable conservation and management effort is needed in the future.     

Electrostatic effect on electron transfer between cytochrome b5 and cytochrome c

The binding and electron transfer between wild type, E44A, E56A, E44/56A, E44/48/56A/D60A and F35Y variants of cytochrome b5 and cytochrome c were studied. When mixed with cytochrome c, the cytochrome b, E44/48/56A/D60A did not show the typical UV-vis difference spectrum of absorption, indicating that the alteration of the surface electrostatic potential obviously influenced the spectrum. The electron transfer rates of wild type cytochrome bj, its variants and cytochrome c at different temperature and ionic strength exhibited an order of F35Y > wild type > E56A > E44A > E44/48/56A/D60A. The enthalpy and entropy of the reaction did not change obviously, suggesting that the mutation did not significantly disturb the electron transfer conformation. The investigation of electron transfer rate constants at different ionic strength demonstrated that electrostatic interaction obviously affected the electron transfer process. The significant difference of Cyt b, F35Y and E44/48/56A/D60A from the wild type protein further confirmed the great importance of the electrostatic interaction in the protein electron transfer.     

尖镰孢菌细胞色素P45055A1与NO相互作用的荧光光谱法研究

以大肠杆菌为宿主表达了尖镰孢菌细胞色素P450 55A1(CYP450 55A1),并采用荧光光谱法研究了其与NO的相互作用.结果表明:在大肠杆菌(DE3)中成功表达了具有酶活性的CYP55A1.在280 nm波长激发下,NO的加入使CYP55A1的荧光逐渐降低,但随温度的变化差异较小;在413 nm波长激发下,NO的加入使CYP55A1的荧光逐渐升高,但加入NADH后其荧光又恢复到原来的值.     

东北粗皮蛙线粒体 DNA 遗传多样性分析

本文通过对东北粗皮蛙29个样本线粒体DNA控制区和细胞色素b基因部分序列分析,探讨其遗传多样性和种群遗传结构。 PCR扩增和测序结果,获得587bp的线粒体DNA控制区序列和895bp的细胞色素b基因序列。合并线粒体DNA序列分析,共定义14个单倍型,16个变异位点,单倍型多样性（H）为0．704,核苷酸多样性（π）为0．001,平均核苷酸差异数为1．438,表明东北粗皮蛙遗传多样性较低。分子变异分析（AMOVA）结果表明,种群内变异占全部遗传变异的98．13％,群体间没有遗传分化（FST ＝0．0187）,因此应加强对东北粗皮蛙野生种群的保护。     

细胞色素C/L-半胱氨酸修饰电极的电化学行为研究

采用自组装修饰法将细胞色素C修饰到以L-半胱氨酸为连接剂的金电极上,并运用循环伏安法(CV)、电化学阻抗法(EIS)等方法研究了该电极的电化学行为.测定了细胞色素C有关的电化学参数.     

红豆草组织培养中不同培养物同工酶谱差异的研究

对红豆草组织和原生质体培养中的不同培养物进行了过氧化物酶、细胞色氧化酶和酯同工酶的研究。结果表明，在胚性与非胚性愈伤组织、再生的正常苗与玻璃苗、再生苗与种子苗之间均存在明显的差异。     

尾式金属卟啉配合物的研究——Ⅱ.新型尾式金属卟啉配合物的催化和载氧功能

分别研究了5-对[4-(间-吡啶氧基)丁氧基]苯基10,15,20-三苯基卟啉的Fe(Ⅱ).Fe(Ⅲ)和Co(Ⅱ)配合物的催化或载氧功能.以合成的金属卟啉、抗坏血酸和底物所组成的体系作为细胞色素P450单加氧酶的模拟体系,研究了在温和条件下对环已烷转变为环己酮、环己醇的催化作用,测定了这些体系的吸氧能力和羟化产物的产率.结果表明,尾式铁卟啉配合物Fe(Ⅲ)PyBPTPPCI 比其他简单相应铁卟啉配合物更为有效.用紫外可见光谱测定了尾式Co(Ⅱ)卟啉配合物的载氧能力,该模型化合物在室温苯溶液中呈现了可逆载氧能力,通过红外光谱进一步证明在氧合金属卟啉中的氧分子是以端基方式与中心金属Co 配位的.     

Roles of Phe58 residue in stabilizing structure of cytochrome b<Subscript>5</Subscript>

To understand effect of π-stacking interactions between the side chain of aromatic amino acids and the porphyrin ring on structures and properties in cytochrome b₅ (cyt b₅), the Phe58 residue was mutated to tyrosine and tryptophan, respectively by site-directed mutagenesis. The denaturation of cyt b₅ F58W and F58Y toward guanidine hydrochloride was examined by UV-visible and fluorescence spectroscopy. The kinetics of heme transfer reactions between apo-myoglobin and the mutants were studied. The results indicated that the mutation of F58 residue for Y58 or W58 reduced the interaction between of peptide and the heme group, resulting in decrease of the T _m and C _m values of the proteins, increase of the heme transfer reaction rate, and shifts of the redox potential.