Molecular mechanisms of lymphatic vascular development

Lymphatic vasculature has recently emerged as a prominent area in biomedical research because of its essential role in the maintenance of normal fluid homeostasis and the involvement in pathogenesis of several human diseases, such as solid tumor metastasis, inflammation and lymphedema. Identification of lymphatic endothelial specific markers and regulators, such as VEGFR-3, VEGF-C/D, PROX1, podoplanin, LYVE-1, ephrinB2 and FOXC2, and the development of mouse models have laid a foundation for our understanding of the major steps controlling growth and remodeling of lymphatic vessels. In this review we summarize recent advances in the field and discuss how this knowledge as well as use of model organisms, such as zebrafish and Xenopus, should allow further in depth analysis of the lymphatic vascular system. Received 26 January 2007; received after revision 5 March 2007; accepted 29 March 2007     

慢性心力衰竭兔心室结构重构对心电生理功能产生的影响及意义

目的 探讨慢性心力衰竭(CHF)兔心室结构重构对心电生理功能的影响及意义.方法 雄性新西兰兔32只,随机分为对照组(n=15)和CHF组(n=17),CHF组采用经兔耳静脉注射异丙肾上腺素(ISO)制备CHF模型,最终造模成功15只.对照组给予相同体积的0.9％生理盐水,最终12只进入实验.采用超声心动图监测两组兔M型...     

北五味子多糖对异丙肾上腺素致大鼠心室重构的保护作用

目的观察北五味子多糖(Schisandra chinensis polysaccride,SCP)抗异丙肾上腺素(Isoproterenol,ISO)诱导的心室重构作用并探讨其作用机制.方法异丙肾上腺素皮下注射致心室重构模型,经SCP低、中、高(25,50,100 mg/kg)3个剂量组和阳性对照卡托普利(50 mg/kg)治疗后,观察其对心肌病理学改变、心室重量(HW/BW)和心室重量指数(LVI)、羟脯氨酸(Hyp)含量、Ⅰ型和Ⅲ型胶原含量及Western blot技术检测心肌组织中TGF-β1表达的影响.结果 SCP不同剂量组均能降低HW/BW和LVI(P0.05或P0.01),降低羟脯氨酸含量(P0.05或P0.01)),降低心肌组织中Ⅰ型和Ⅲ型胶原含量(P0.05或P0.01或P0.001),减轻TGF-β1在心肌组织中的表达(P0.05或P0.01或P0.001),抑制心室重构.结论 SCP能够通过抑制TGF-β1的表达来对抗ISO诱导的大鼠心室重构.     

冲击荷载作用下饱和粉土孔压发展规律及液化特性研究

对四川三溪村滑坡地区的覆盖层粉土进行试验研究,在循环周期荷载作用下通过改变振动频率等主观因素,分析其饱和重塑粉土液化特性.通过改变外界条件,包括围压、动应力以及振动频率等多个因素,研究饱和粉土的孔压变化,进而分析在不同冲击荷载作用下,粉土的液化程度.试验结果表明,在振动频率为5 Hz时,粉土极易发生液化,当频率在其他范围时则明显看出其液化程度降低;在同一围压下,粉土孔压随动荷载的增大呈现出逐渐降低的趋势,其液化程度逐渐降低.     

合肥市"城中村"和谐治理改造的策略

文章研究了"城中村"的一般特点,"城中村"的发展具有明显的阶段性。如果能在其发展的早期对它进行政策上的管理和引导,将大大降低"城中村"改造的难度和成本。以合肥市为例,从统筹规划、管理体制、集体资产的处理、利益分配机制和组织宣传工作等方面阐述了早期"城中村"治理改造中的对策,试图为中等发达城市的"城中村"和谐改造提供参考和借鉴。     

废弃矿区景观重塑探讨——以海口磷矿为例

矿产资源的开发利用形成了大量废弃矿区,严重破坏了矿山生态环境。我国矿山企业虽做了很多的恢复治理工作,但成效都不十分明显。对废弃矿区景观重塑问题进行研究,帮助企业走出这一困境已经势在必行。以云南昆明海口磷矿的废弃矿区为例,在景观生态学的相关理论的基础上对废弃矿区景观重塑展开探讨,对景观生态进行重新规划设计。对我国各类矿山废弃矿区的生态恢复及景观设计具有一定的参考价值。     

Chromatin domain boundaries in the Bithorax complex

Eukaryotic chromosomes are thought to be organized into a series of discrete higher-order chromatin domains. This organization is believed to be important not only in the compaction of the chromatin fibre, but also in the utilization of genetic information. Critical to this model are the domain boundaries that delimit and segregate the chromosomes into units of independent gene activity. In Drosophila, such domain boundaries have been identified through two different approaches. On the one hand, elements like scs/scs′ and the reiterated binding site for the SU(HW) protein have been characterized through their activity of impeding enhancer-promoter interactions when intercalated between them. Their role of chromatin insulators can protect transgenes from genomic position effects, thereby establishing in dependent functional domains within the chromosome. On the other hand, domain boundaries of the Bithorax complex (BX-C) like Fab-7 and Mcp have been identified through mutational analysis. Mcp and Fab-7, however, may represent a specific class of boundary elements; instead of separating adjacent domains that contain separate structural genes, Mcp and Fab-7 delimit adjacent cis-regulatory domains, each of which interacts independently with their target promoters. In this article, we review the genetic and molecular characteristics of the domain boundaries of the BX-C. We describe how Fab-7 functions to confine activating as well as repressive signals to the flanking regulatory domains. Although the mechanisms by which Fab-7 works as a domain boundary remain an open issue, we provide preliminary evidence that Fab-7 is not a mere insulator like scs or the reiterated binding site for the SU(HW) protein.