氢化物—原子荧光法测定人血清中的硒

本文研究了用湿法消化样品，氢化物—原子荧光法测定人血清中硒含量最佳条件。线性范围０—４０．０ｎｇ／ｍＬ．检测限为１．０ｎｇ／ｍＬ相对标准偏差为３．５％，平均回收率为９８．２％。用该法对合肥地区２４１例正常人的血清硒水平做了检测评估。     

毕赤酵母表达硒代重组人血清白蛋白的研究

本试验将能够成功表达重组人血清白蛋白(rHSA)的巴斯德毕赤酵母培养至诱导期后，流加亚硒酸钠，同时设空白对照发酵液(不加入亚硒酸钠)，通过对比相同菌株在两组培养基中表达的rHSA的含硒量，判断硒能否以硒代氨基酸的形式存在于重组蛋白质中。发酵液经热处理、超滤、离子交换和丙酮沉淀等步骤后，进行Tricine-SDS-PAGE凝胶电泳、X射线能量色散谱(EDX)和原子吸收分析。结果表明，加硒发酵液样品中硒元素的质量分数比对照多23.2%，硒与蛋白质的质量比为0.0069，高于对照发酵液近35倍，初步证明无机硒可以被毕赤酵母同化，转化为硒代氨基酸，并形成硒代重组蛋白质。     

Spectroscopic studies on interaction of hemoglobin and serum albumin with nicotine

The interactions of nicotine and Hb/SA were studied in vitro by UV/Vis, fluorescence, 1H NMR and FT-IR spectroscopies. The UV/Vis absorbance of Hb/SA (200 nm)shifted to red and decreased gradually with the addition of nicotine, indicating that the protein conformational change resulted from the chemical interaction. With increasing nicotine concentration, incubation of SA with nicotine caused the quenching of fluorescence typical of protein tryptophan residues, which meant that the vicinity of the tryptophan residues of SA was changed because of nicotine. FT-IR spectra showed that α-helix component of Hb/SA decreased, turn and β-structure components of Hb/SA increased in the presence of nicotine. In the 1H NMR spectra of nicotine, all proton peaks on pyrrolidinyl ring moved to downfield and the resonance emanating from nicotine was preferentially broadened while the concentration of Hb/SA increased. All these results indicate that nicotine and Hb/SA in vitro interact on each other, forming a new complex and inducing the protein conformational change.     

奶牛精子尾部颈段的超微结构

奶牛精子尾部颈段的中央结构是连接片(connecting piece).连接片具横纹,由踝状突(capitulum)和9条节柱(segmented columns)构成。踝状突位于核后端的植入窝中。踝状突的腹面与节柱相连。有3条节柱在近踝状突腹面处聚集成为一条较大的节柱,与踝状突前端的腹面相连。另有2条节柱也在近踝状突腹面聚集成为另一条较大的节柱,与踝状突后端的腹面相连。其余4条节柱与踝状突两侧的腹面相连。9条节柱的末端分别与9条外周致密纤维(outer dense fibers)相连。近端中心粒位于踝状突之中,远端中心粒分裂成为9条柱状结构。每条柱状结构分别依附于每一条节柱与外周致密纤维连接处的内侧。在远端中心粒的中央有轴丝的两条中央微管和两条内棒(internal robs)。在连接片的外侧方还有线粒体。线粒体不形成线粒体鞘。     

Deficiency of fibrinolytic enzyme activities in the serum of patients with Alzheimer-type dementia

Previously we reported that there is a kallikrein deficiency in the cerebral tissue of patients with Alzheimer-type dementia. The present study was performed to investigate protease changes in the serum of these patients. The results showed that the kallikrein activity was normal, but that the activities of plasmin and urokinase were significantly low. The present findings indicate a derangement in the clotting and fibrinolytic systems in Alzheimer patients.     

不同运动形式对血清睾酮的影响

本文的研究目的是调查体育系和其他男大学生在耐力练习(1500m)和力量练习时的血清睾酮浓度的反应及差异,以探讨不同体能水平,不同运动形式对运动时垂体—睾丸系统的影响。     

川西北草地牛皮蝇蛆病的研究

对川西北草地牛皮蝇蛆病的流行情况、季节动态及防治技术的研究表明：（１）临床检查牦牛１１４９头、黄牛５９９头，皮蝇蛆寄生率分别为８４．２５％和７０．２８％，寄生强度为１８．３２个和２．１７个；剖杀检查牦牛１１４头，皮蝇蛆寄生率为１００％，寄生强度为７１．６６个．（２）川西北草地皮蝇的种类，经形态学和生化特性鉴定定为牛皮蝇Ｈ．ｂｏｖｉｓ、纹皮蝇Ｈ．ｌｉｎｅａｔｕｍ和中华皮蝇Ｈ．ｓｉｎｒｎｓｅ等三种．（３）弄清了皮蝇各期幼虫在牛体内的寄生季节、寄生部位、移行动态、三期幼虫寄生方位、入土化蛹羽化规律、成虫活动规律等．（４）害获灭（Ｉｖｏｍｅｃ）是防治牛皮蝇蛆病的首选药物，具有效果好、广谱、剂量小、安全、使用方便、易于推广等优点，防治后可获显著经济效益和明显社会效益     

牦牛群体血清运铁蛋白位点遗传分化的探讨

根据中国，蒙古，前苏联等３国９个牦牛地方品种（类群）血清运铁蛋白位点的基因频率，探讨了各牦牛群体的遗传分化程度，结果表明，血清运铁蛋白各等位基因频率在不同的牦牛群体中，具有明显的种群特异性，中国牦牛品种（类群）间遗传分化程度大，而蒙古牦牛品种（类群）间遗传分化程度较低，这反映了牦牛起源进化上的差异。