板蓝根泡腾片浸膏中腺苷和(R,S)-告依春的含量测定

目的:建立板蓝根泡腾片浸膏中腺苷和(R,S)-告依春含量的高效液相色谱测定方法。方法:高效液相色谱仪Waters 2695-2487;Diamonsil C18柱(250 mm×4.6 mm,5μm);柱温:30℃;流动相:甲醇-水(10:90);流速为1.0mL/min;检测波长:255nm。结果:精密度和稳定性均良好,腺苷的质量浓度在1.012μg/mL~30.36μg/mL范围内,线性关系良好,r=1,平均回收率为96.7%,RSD为1.1%;(R,S)-告依春的质量浓度在2.042μg/mL~61.26μg/mL范围内,线性关系良好,r=1,平均回收率为97.9%,RSD为0.7%。结论:该法简便快速,结果准确,重复性好,可作为板蓝根泡腾片浸膏的质量控制方法。     

N,N-二苄基-L-谷氨酸及其金属配合物的合成、表征及生物活性

以L-谷氨酸为原料合成了N,N-二苄基-L-谷氨酸（H2bbga）,并对其进行了红外光谱、紫外光谱、核磁共振表征.通过N,N-二苄基-L-谷氨酸与MgCl2.6H2O、Ca（OH）2、MnCl2.4H2O、NiCl2.6H2O、CoCl2.6H2O、CuCl2.2H2O和ZnCl2反应,分别合成得到了相应的金属配合物.用比色法测试了N,N-二苄基-L-谷氨酸及7种金属配合物对三磷酸腺苷二钠分解的催化活性,结果表明：N,N-二苄基-L-谷氨酸与镁、钙和锰形成的配合物对三磷酸腺苷二钠的分解反应具有较好的活性.     

辛基酚聚氧乙烯醚磷酸酯的合成研究与性能测定

:研究了辛基酚聚氧乙烯醚磷酸酯的合成工艺 ,选出了较佳的工艺条件为 nop- 10 ∶ n P2 O5 为2 .3∶ 1 ,反应温度为 70℃ ,反应时间为 4h,单酯收率可达 93.2 8%。性能测定表明 ,它是性能优良的表面活性剂     

Effect of pyrophosphate and orotidine monophosphate on cytosine deaminase regulatory properties

Summary The maximal velocity of the reaction (V_max) and the half-saturation constant (K_0.5) values of theS. typhimurium cytosine deaminase were altered in the presence of its effectors, pyrophosphate and orotidine monophosphate. From the kinetics of orotidine monophosphate inhibition of cytosine deaminase, it was characterized as a mixed-type noncompetitive inhibitor.     

活性小分子的辐射效应

研究结果表明:1.600R 注 X 线照射后,小白鼠肝中谷胱甘肽(GSH)浓度下降50％(P<0.01)、脾中增高36％(P<0.05)、肾中下降15.9％(P<0.05).2.当小白鼠照射前注射 GSH 或半胱氨酸(CySH)能稳定 SH 基水平.3.采用放射免疫技术(RIA)测定小白鼠血清胰岛素浓度,照射后16小时降低22.5％.血糖浓度照射后18小时下降9％.血清环化腺苷单磷酸(cAMP)浓度照后16小时下降31％.4.不同剂量γ线照射后,鼠脑含磷化合物的合成受到强烈抑制.5.6000R γ线照射后兔的通透屏障受到明显影响.     

Renal vascular reactivity to ATP in hyper- and hypothyroid rats

The effects of adenosine triphosphate (ATP) on the renal vasculature of isolated kidneys from control, hyper- and hypothyroid rats were characterized. ATP responsiveness was evaluated in basal tone and in raised tone (phenylephrine 10^–6 M) preparations. These responses were compared with those obtained with barium chloride or sodium nitroprusside (SNP), used respectively as nonreceptor agonists for vasoconstriction or vasodilation. In preparations at basal tone, ATP produced dose-related vasoconstriction, which was increased in hyperthyroid kidneys, and was severely attenuated in kidneys from hypothyroid rats. In raised tone preparations from control rats ATP produced a dual response: vasoconstriction at low doses, which declined with increasing doses to give way to vasodilator responses; biphasic responses were found in some kidneys. Hyperthroid kidneys showed increased pressor responses and a vasodilator response similar to those seen in kidneys from control rats. However, in hypothyroid kidneys the vasodilator response was abolished. The responses to barium chloride and to SNP were significantly increased and decreased in hyper- and hypothyroid kidneys, respectively; vasoconstrictor responses to SNP were also found in hypothyroid kidneys. Hence the abnormal responses to ATP observed in both thyroid dysfunctions may be partially explained by unspecific alterations in the contractile machinery of the renal vasculature in these kidneys. However, ATP responsiveness (vasoconstriction at low tone and vasodilation at raised tone) was more severely affected in hypothyroid kidneys, suggesting that purinergic (P_2X and P_2Y) receptor activity may be decreased in these organs.     

Oviposition stimulants of aCitrus-feeding swallowtail butterfly,Papilio xuthus L

Summary A methanolic extract ofCitrus unshiu induces oviposition by females of aCitrus-feeding swallowtail butterfly,Papilio xuthus L. The chemical factors responsible for stimulating oviposition were isolated and characterized as 5-hydroxy-N-methyltryptamine, adenosine, vicenin-2, narirutin, hesperidin and rutin. An artificial blend of these six components elicited significant oviposition behavior, apparently identical to that induced by contact with intactCitrus leaves.     

低速低压温度敏感硼酸亲和色谱制备

利用原子自由基转移聚合原理,制备聚(N-异丙基丙烯酰胺-co-4-乙烯基苯硼酸)改性的温度敏感硼酸亲和色谱固定相,并对其进行傅里叶红外光谱,X射线光电子能谱等结构表征以及用热失重对其接枝率进行了测定.用HPLC研究了腺苷和脱氧腺苷的保留行为.并且应用于空间生命仪器搭载装置的分离装置中,在不同温度下对腺苷进行富集并对其收集液进行分析.结果表明,在水溶液为流动相的条件下,可以实现在低流速、低压的条件下对腺苷的富集,且回收率为85.4%.     

钙对甘薯种苗盐胁迫的缓解效应

用２０ｍｍｏｌ／ＬＣａＣｌ２溶液浸种能提高甘薯种子在盐胁迫下的萌发率，改善甘薯种苗的生长状态，减轻甘薯胚根在盐胁迫下的膜伤害，钙处理后，甘薯种苗在盐胁迫下ＡＴＰ含量，游离脯氨酸含量增加，这可能是钙具有缓解甘薯盐迫作用的生理基础。