关于完备格成分理论中两个问题的探讨

研究了完备格成分理论中两个尚未解决的问题：（i)在非分配的完备格中,有限宽元素的成分也是有限宽的吗？（ii)什么样的映射能保持颗粒表示性质或颗粒性质？通过构造反例证明了在非分配的完备格中,有限宽元素的成分不必是有限宽的;还证明了完备格间的同构映射能保持颗粒表示性质和颗粒性质,通过反例证明了同构映射的条件不能减弱,从而解决了以上两个问题。     

超声处理后的玉米淀粉与环氧丙烷的反应机理

研究了玉米淀粉经超声处理后的化学反应性能变化。将经超声处理的玉米淀粉与环氧丙烷进行反应,发现反应取代度（Ds）由未处理时的0．113提高到0.225以上,同时发现提高超声功率对反应取代度有一定的促进作用,但随着作用时间的延长,反应取代度提高不明显。采用偏光显微镜研究超声处理后玉米淀粉的结构,发现超声处理后玉米淀粉整个颗粒的结晶结构变化不明显;用扫描电镜观察发现部分淀粉颗粒表面出现大小不同的小孔,部分小孔分布十分均匀,呈蜂窝状,由此推断超声对淀粉的作用只发生在淀粉颗粒表面的无定形区。     

The effect of a secretion-enhanced heavy chain on improving intein-based dual-vector co-delivery of a full-length factor VIII gene

Treatment of hemophilia A by gene therapy is adversely affected by inefficient FVIII secretion and the large FVIII gene, which is difficult to package in the promising adeno-associated virus (AAV) vectors. Inhibited secretion of FVIII is caused mainly by inefficient secretion of its heavy chain. Previously, we have employed a protein splicing-based dual-vector to co-transfer a B-domain-deleted FVIII (BDD-FVIII) gene, suggesting that the light chain, covalently ligated to a co-expressed heavy chain can improve the secretion of spliced BDD-FVIII. However, its level of secretion was affected by inefficient secretion the heavy chain. Here, we studied the effect of a mutant heavy chain with L303E/F309S substitutions, which enhance FVIII secretion on the heavy chain itself and spliced FVIII when using a protein splicing-based split-delivery of a full-length FVIII gene. Eukaryotic vectors expressing Ssp DnaB intein-fused mutant heavy and light chains were transiently co-transfected into cultured COS-7 cells. A spliced FVIII protein was seen in co-transfected cells by Western blot analysis. The heavy chain was secreted by cells transfected with the mutant heavy chain gene alone at (39±11) ng/mL and this secretion increased to (123±13) ng/mL when cells were co-transfected with the light chain gene, which was greater than the secretion of wild-type heavy chain. The amount of spliced FVIII in the culture supernatant of co-transfected cells was (86±14) ng/mL, with an activity of (0.61±0.08) IU/mL, which was greater than that of wild-type FVIII co-transfected cells. Spliced FVIII and bioactivity were also detected in the combined culture supernatant of cells individually transfected with mutant heavy and light chain gene at a higher level than that of combined wild-type heavy and light chain transfections. This suggested that the heavy chain with improved secretion markedly increased the efficacy of protein splicing-based split delivery of the full-length FVIII gene using a dual-vector. These results encourage the transfer of this technology to an animal model using a dual-AAV vector.     

彩色图像的球形粒计算分割算法

针对基于聚类的彩色图像分割算法速度较慢,提出了彩色图像的球形粒计算分割算法.将彩色图像每个像素点表示为以该点RGB像素值为中心0为半径的球形粒,设计球形粒之间的合并算子,利用粒度阈值对两球形粒进行有条件合并,得到不同粒度的球形粒组成的球形粒集,以球形粒中心对应的RGB值代替球形粒包含像素点的RGB值.实验结果表明:与K-means算法和FCM算法相比,球形粒计算分割算法是稳定的而且分别加快了6倍和34倍.     

开口箭对小鼠5-羟色胺分泌的影响

目的:采用灌胃、石蜡切片、免疫组化染色和ELISA等方法,探讨开口箭对小鼠5-羟色胺(5-HT)分泌的影响.方法选取20²2g健康昆明小鼠20只,均分成A,B,C,D 4组,分别以蒸馏水和0.25、0.50、1.00mg/mL的开口箭灌胃,0.20mL/次/d,连续灌胃7d.第8d对全部小鼠摘眼球取血,ELISA检测血清5-HT质量浓度;取血后立即处死小鼠,取十二指肠,4%甲醛固定,石蜡切片,免疫组化染色,镜下统计5-HT阳性细胞数量.结果与A组相比,B,C,D组小鼠血液5-HT质量浓度逐渐降低,十二指肠肠壁组织中5-HT阳性细胞数也明显减少.结论开口箭会下调小鼠5-HT的分泌.     

十味龙胆花颗粒治疗痰热壅肺证临床疗效观察

目的：观察十味龙胆花颗粒治疗痰热壅肺证的临床疗效.方法：将入选病人随机分为治疗组和对照组,治疗组服用十味龙胆花颗粒,对照组服用芩暴红止咳颗粒.观察治疗前后中医证候积分变化情况,评价十味龙胆花颗粒治疗痰热壅肺证的临床疗效.结果：中医证候疗效分析十味龙胆花颗粒组临床痊愈74例,显效66例,有效22例,无效4例,痊愈率44.578%,愈显率84.337%;芩暴红止咳颗粒组痊愈16例,显效26例,有效11例,无效3例,痊愈率28.571%,愈显率75.00%.总痊愈率十味龙胆花颗粒组优于芩暴红止咳颗粒组（P<0.05）.主要症状疗效分析：十味龙胆花颗粒组咳嗽症状痊愈95例,显效10例,有效51例,无效11例,痊愈率57.229%,愈显率63.253%;芩暴红止咳颗粒组咳嗽症状痊愈18例,显效6例,有效20例,无效12例,痊愈率32.143%,愈显率42.857%.十味龙胆花颗粒组优于芩暴红止咳颗粒组（P<0.05）.结论：十味龙胆花颗粒有较好的止咳作用,对痰热壅肺证有良好的临床疗效.     

玉门市老君庙油田土壤石油污染状况调查

为探明老君庙油田土壤石油污染情况,采用索氏抽提-重量法分别测定了该油田4个采样区3个土样层面(表层、下层及底层)土壤的含油量,并对其在土壤中的迁移规律进行了初步研究.结果表明,老君庙油田土壤石油污染非常严重,且土壤含油量随土壤粒径减小而增大,最高可达83 601 mg/kg.尽管石油污染物在土壤中存在下渗现象,但是由于黄土对其吸附能力较强,使其主要集中于土壤浅层.     

多丝切割的“滚-刻-削”混合加工机理

当前多丝切割的环境并非理想,加工要求并非相同.而理想及单一的“滚-刻”切割加工模型对整个过程难以解释周全.基于多丝切割中磨粒运行存在多种状态,指出研磨粒的综合力学行为(研磨粒的加工机理)取决于研磨液膜的流体力学条件,处于半接触和全接触状态下的磨粒,各自以“滚-刻”、“滚-刻-削”方式实施切割加工.故多模型混合加工可以较好地解释多丝切割加工的整个过程,多模型的混合加工模式更加接近实际状况.     

粒结构关联度算法在专业辅助选择系统中的应用

本科生入学后均有具体专业的二次选择的机会.建立一套面向大学二年级本科生院内具体专业方向的二次选择的科学辅助评价系统软件,辅助他们较科学地选择正确的专业方向,具有重要的实际意义.该系统广泛研究当代相关的评测理论并且结合本专业特点,建立了一套面向计算机专业大学生的评价体系.针对上述评价体系信息系统,提出了将信息粒度思想与关联规则算法相结合的粒结构关联度算法.基于上述理论研究,研发和实现了一套二次专业方向辅助选择系统.实际应用表明,该系统具有新颖性、可用性和较好的应用价值.     

VS2005环境的动态显微颗粒分析软件开发

为实现动态连续地采集显微颗粒图像,采用CCD高速摄像头连续拍摄显微颗粒图像的方法,并使用VS2005集成环境下的VC.NET编程工具开发动态显微颗粒分析软件.该软件由于考虑了显微颗粒图像采集的动态性和连续性,从而能够实时进行颗粒分析.经实践验证,该软件可应用于各种粉体材料的实时检测.