Infection of SARS-CoV on juvenile and adult Brandt’s vole Microtus brandtii

We studied the infectious effect of SARS-CoV virus on juvenile and adult Brandt‘s Vole (Microtus brandtii) by nasal cavity spraying method (CCIDso is 10^5.7). SARS virus caused serious deaths in adults. The death adults demonstrated hemorrhage from mouth, nasal cavity and intestine, hemorrhageious interstitial pneumonia and gore in liver, spleen and kidney. The survival adults demonstrated local hemorrhagic spot in lung and emphysema, but the other organs showed no pathological abnormality. SARS virus caused no deaths in juveniles, but locomotion of infected juveniles became slower. In the early stage, there was local pneumonia in lung and SARS viruses were isolated from the pathological tissue. Only one control juvenile lived and the infected juvenile showed local pneumonia in lung. The results demonstrated that SARS-CoV infected Brandt‘s vole seriously and adults were more susceptive to SARS-CoV than juveniles. The Brandt‘s vole may be a potential animal model for SARS research.     

大宗农产品供应链四维网络模型及应用

为探索建立有效大宗农产品供应链的分析方法,建立了供应链四维网络模型,提出了改进供应链的分析原则,以此分析了黑龙江垦区水稻供应链,总结了建立大宗农产品有效供应链的主要约束,表明以分布式协作管理供应链、以质量安全标准集成供应链是有效的管理方法.     

Construction of single-chromosome DNA library fromLilium regale Wilson

A protocol of simple rapid microdissection of single-chromosome, amplification and cloning of its DNA fromLilium regale Wilson is described. Single-chromosome, microdissected by micromanipulator, was put into a 0.5 mL Eppendorf tube and digested with Sau3A, and then the Sau3A linker adaptors were ligated to the ends of DNA fragments. After 2 rounds of PCR amplification with one chain of linker adaptor as primer, the PCR products thus obtained have a length of 300–2500 base pairs (bp) with predominant fragments at about 1000 bp. Southern blot analysis confirmed that the PCR products originated from the genome ofLilium regale Wilson. By cloning the amplification products from the second round of PCR, single-chromosome DNA library was constructed, in which about as many as 100000 recombinant clones were produced. A total number of 84 clones were analysed, and it was revealed that the inserts ranged in size from 300 to 1800 bp, with an average of780 bp. Compared with the methods described in other literature, this protocol, eliminating the need for enzymatic digestion and ligating micromanipulation of chromosomal DNA in nanoliter volumes, permits the efficient amplification of single chromosome (not tens of chromosomes as reported before) and the fragments (780 bp in average) cloned in this study are longer than those reported before (650 bp in average).     

混合所有制企业监事会治理能力评价及建设

混合所有制企业监事会治理能力个体优势互补是发展混合所有制经济的必要条件,判别其治理能力的个体优势是优势互补的重要基础.根据竞优的思想,从被评估企业最有利的视角确定价值参数,提出了混合所有制企业监事会治理能力的个体优势判别模型,同时给出模型的解法.采用了我国第一批试点的10家混合所有制企业作为研究样本,根据本文的基于个体优势的监事会治理能力评价方法进行了计算实例分析,同时提出对策建议,为其科学评价提供参考.     

(2+1)-维Toda-like晶格方程的对称变换和精确解

基于符号计算软件Maple,利用楼直接方法研究了一个(2+1)-维Toda-like晶格方程的对称变换。基于求得的对称变换,得到了这个微分差分方程一个新的类孤子解。该方法对于求解微分差分方程十分有效,并可以获得丰富的精确解。     

Schwinger模型中矢量粒子质量的格点计算

本文用变分方法计算带Wilson费米子的格点Schwinger模型的矢量介子的质量,得到与连续理论严格解相符的结果。     

特殊正交各向异性压电弯曲板的精化理论

对特殊正交各向异性压电材料进行了精化分析,给出了该材料板弯曲时的精化理论。首先,介绍特殊正交各向异性压电材料满足的基本方程和通解,并将调和函数的算子函数表示推广到椭圆广义调和函数。其次,利用算子函数表示将板内的位移场、电势场、应力场和电位移场利用二维函数表示出来。然后,利用非齐次边界条件,获得该板在作用横向载荷时的精化方程。最后,对精化方程进行分析,略去高阶项后,得到了特殊正交各向异性压电弯曲板作用横向载荷时的近似方程。由于该研究方法没有进行预先假设,所以获得的结果比一般的板变形理论更精确。     

积分中值定理中间点的渐近估计式

利用在无穷区间上的比较函数概念,在g(x)可积的较弱条件下,建立了第一、二积分中值定理"中间点"当x→+∞时更广泛的渐近估计式,作为推论得到了Cauchy中值定理和Taylor中值定理的"中间点"当x→+∞时的渐近估计式,从而统一和发展了有关文献的结果.     

关于矿山救护队自身伤亡问题的探讨

造成矿山救护队自身伤亡的原因,包括主观因素和客观因素两个方面。通过结合典型案例,在深入分析决策失误、违章救援、技术措施不当、呼吸器故障和身体素质不达标等致因因素的基础上,基于"管理、装备、培训"三并重原则,就如何有效避免自身伤亡事故的发生,分别从科学的救援理念、正确的决策指挥、合理可行的救援方案、先进适用的救援技术装备、科学严格的管理和训练以及培育安全救护文化等方面提出了针对性建议措施。     

Robust SLAM using square-root cubature Kalman filter and Huber’ s GM-estimator

Mobile robot systems performing simultaneous localization and mapping ( SLAM) are generally plagued by non-Gaussian noise.To improve both accuracy and robustness under non-Gaussian meas-urement noise, a robust SLAM algorithm is proposed.It is based on the square-root cubature Kal-man filter equipped with a Huber’ s generalized maximum likelihood estimator ( GM-estimator) .In particular, the square-root cubature rule is applied to propagate the robot state vector and covariance matrix in the time update, the measurement update and the new landmark initialization stages of the SLAM.Moreover, gain weight matrices with respect to the measurement residuals are calculated by utilizing Huber’ s technique in the measurement update step.The measurement outliers are sup-pressed by lower Kalman gains as merging into the system.The proposed algorithm can achieve bet-ter performance under the condition of non-Gaussian measurement noise in comparison with benchmark algorithms.The simulation results demonstrate the advantages of the proposed SLAM algorithm.