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251.
YU FuDong KANG Bin LI YuanYuan LI YiXue 《科学通报(英文版)》2007,52(15):2100-2107
Schistosomes cause schistosomiasis disease which severely threatens human health. Little is known about the functions of EF-hand domain containing schistosomes tegument proteins other than as an-tigens. More possible functions of these tegument proteins were investigated with in silico analyses including protein-protein functional interaction,site-specific variation and glycosylation modification. The analysis results suggested that schistosomes could actively modulate host immune responses for its own favor through functional interactions with host proteins with immunomodulatory function,and passively regulate host immune responses through sequence variation under positive selection and glycosylating the recognition sites of host immune attack. In addition,the analysis of the C-terminal domain of these tegument proteins indicated that they could assist schistosomes in escaping host immune attacks through inhibiting chemotaxis and non-complement fixing antibody (IgG4) responses. In summary,our results suggested that these tegument antigen proteins could assist schistosomes in escaping and modulating host immune responses for self-protection during the process of host-para-site interaction. 相似文献
252.
Decreased pulmonary levels of the anti-inflammatory Clara cell 16 kDa protein after induction of airway inflammation in asthmatics 总被引:13,自引:0,他引:13
Lensmar C Nord M Gudmundsson GH Roquet A Andersson O Jörnvall H Eklund A Grunewald J Agerberth B 《Cellular and molecular life sciences : CMLS》2000,57(6):976-981
The Clara cell 16 kDa protein (CC16) maps to an atopy-associated region of chromosome 11 and has been ascribed an anti-inflammatory
function. Using reverse-phase HPLC and Western blot analysis, we have evaluated the polypeptide pattern in bronchoalveolar
lavage (BAL) fluid retrieved from asthmatics, before and after induction of airway inflammation by low-dose allergen inhalation
challenge. A prominent decrease of CC16 was seen after induction of inflammation, and a further CC16 decrease was observed
in lavage fluid where surfactant had been removed. Reduced levels of pulmonary CC16 may cause loss of anti-inflammatory activity
in the airways and contribute to the development of airway inflammation in asthma.
Received 22 March 2000; received after revision 4 May 2000; accepted 4 May 2000 相似文献
253.
N. Soltani S. Chebira J. P. Delbecque J. Delachambre 《Cellular and molecular life sciences : CMLS》1993,49(12):1088-1091
Flucycloxuron, a novel benzoylphenylurea (BPU) derivative, exhibited insecticidal activity when injected into newly ecdysed pupae ofTenebrio molitor. Mortality occurs because of defective adult ecdysis. Treatment caused a reduction in both cuticle thickness and incorporation of14C-labelled precursor into chitin, although it had no significant effect on the protein synthesis. The potencies of other BPU compounds as inhibitors of chitin biosynthesis have been examined and results showed that diflubenzuron was less effective than either flucycloxuron or triflumuron. 相似文献
254.
255.
The functional significance of the lipid-protein interface in photosynthetic membranes, mainly in thylakoids, is reviewed with emphasis on membrane structure and dynamics. The lipid-protein interface is identified primarily by the restricted molecular dynamics of its lipids as compared with the dynamics in the bulk lipid phase of the membrane. In a broad sense, lipid-protein interfaces comprise solvation shell lipids that are weakly associated with the hydrophobic surface of transmembrane proteins but also include lipids that are strongly and specifically bound to membrane proteins or protein assemblies. The relation between protein-associated lipids and the overall fluidity of the thylakoid membrane is discussed. Spin label electron paramagnetic resonance spectroscopy has been identified as the technique of choice to characterize the protein solvation shell in its highly dynamic nature; biochemical and direct structural methods have revealed an increasing number of protein-bound lipids. The structural and functional roles of these protein-bound lipids are mustered, but in most cases they remain to be determined. As suggested by recent data, the interaction of the non-bilayer-forming lipid, monogalactosyldyacilglycerol (MGDG), with the main light-harvesting chlorophyll a/b-binding protein complexes of photosystem-II (LHCII), the most abundant lipid and membrane protein components on earth, play multiple structural and functional roles in developing and mature thylakoid membranes. A brief outlook to future directions concludes this review. 相似文献
256.
Heterologous expression of G-protein-coupled receptors: comparison of expression systems from the standpoint
of large-scale production and purification 总被引:14,自引:0,他引:14
Sarramegna V Talmont F Demange P Milon A 《Cellular and molecular life sciences : CMLS》2003,60(8):1529-1546
G-protein-coupled receptors (GPCRs) are of prime importance for cell signal transduction mechanisms and are the target of many current and potential drugs. However, structural data on these membrane proteins is still scarce because of their low natural abundance and the low efficiency of most of the expression systems currently available. This review presents the most important expression systems currently employed for heterologous expression of GPCRs; Escherichia coli, yeast, insect cells and mammalian cells. After briefly recalling the specificity, advantages and limitations of each system, particular emphasis is put on the quantitative comparison of these expression systems in terms of overall expression yield, and on the influence of various factors (primary sequence, origin, cell type, N- and C-terminal tags) on the results. 相似文献
257.
Popot JL Berry EA Charvolin D Creuzenet C Ebel C Engelman DM Flötenmeyer M Giusti F Gohon Y Hong Q Lakey JH Leonard K Shuman HA Timmins P Warschawski DE Zito F Zoonens M Pucci B Tribet C 《Cellular and molecular life sciences : CMLS》2003,60(8):1559-1574
Membrane proteins classically are handled in aqueous solutions as complexes with detergents. The dissociating character of detergents, combined with the need to maintain an excess of them, frequently results in more or less rapid inactivation of the protein under study. Over the past few years, we have endeavored to develop a novel family of surfactants, dubbed amphipols (APs). APs are amphiphilic polymers that bind to the transmembrane surface of the protein in a noncovalent but, in the absence of a competing surfactant, quasi-irreversible manner. Membrane proteins complexed by APs are in their native state, stable, and they remain water-soluble in the absence of detergent or free APs. An update is presented of the current knowledge about these compounds and their demonstrated or putative uses in membrane biology. 相似文献
258.
Protein folding and degradation in bacteria: to degrade or not to degrade? That is the question 总被引:11,自引:0,他引:11
In Escherichia coli protein quality control is carried out by a protein network, comprising chaperones and proteases. Central to this network
are two protein families, the AAA+ and the Hsp70 family. The major Hsp70 chaperone, DnaK, efficiently prevents protein aggregation
and supports the refolding of damaged proteins. In a special case, DnaK, together with the assistance of the AAA+ protein
ClpB, can also refold aggregated proteins. Other Hsp70 systems have more specialized functions in the cell, for instance HscA
appears to be involved in the assembly of Fe/S proteins. In contrast to ClpB, many AAA+ proteins associate with a peptidase
to form proteolytic machines which remove irreversibly damaged proteins from the cellular pool. The AAA+ component of these
proteolytic machines drives protein degradation. They are required not only for recognition of the substrate but also for
substrate unfolding and translocation into the proteolytic chamber. In many cases, specific adaptor proteins modify the substrate
binding properties of AAA+ proteins. While chaperones and proteases do not appear to directly cooperate with each other, both
systems appear to be necessary for proper functioning of the cell and can, at least in part, substitute for one another.
RID="*"
ID="*"Corresponding author. 相似文献
259.
Evidence of undiscovered cell regulatory mechanisms: phosphoproteins and protein kinases in mitochondria 总被引:3,自引:0,他引:3
Thomson M 《Cellular and molecular life sciences : CMLS》2002,59(2):213-219
The finding that mitochondria contain substrates for protein kinases lead to the discovery that protein kinases are located
in the mitochondria of certain tissues and species. These include pyruvate dyhydrogenase kinase, branched-chain α-ketoacid dehydrogenase kinase, protein kinase A, protein kinase Cδ, stress-activated kinase and A-Raf as well as unidentified kinases. Recent evidence suggests that mitochondrial protein kinases
may be involved in physiological processes such as apoptosis and steroidogenesis. Additionally, the novel finding of low-molecular-weight
GTP-binding proteins in mitochondria suggests the possibility that these may interact with mitochondrial protein kinases to
regulate the activity of mitochondrial effector proteins. The fact that there are components of cellular regulatory systems
in mitochondria indicates the exciting possibility of undiscovered systems regulating mitochondrial physiology.
Received 19 June 2001; received after revision 7 August 2001; accepted 8 August 2001 相似文献
260.
杨昌山 《暨南大学学报(自然科学与医学版)》2002,23(4):20-23
自从杆状病毒中分离出凋亡蛋白抑制剂 (inhibitorofapoptosisproteins ,IAPs)后 ,发现的凋亡蛋白抑制剂的种类和数量逐渐增多。迄今为止 ,在人体新发现的IAPs有HIAP - 1(humanIAP -1)、HIAP - 2 (humanIAP - 2 )、XIAP(Xchromosome -likedIAP)、ML -IAP(melanocytesIAP)、Survivin和Livin等。对IAPs的发现、定位、结构、分布和作用等方面的研究进展进行介绍 相似文献