全文获取类型
收费全文 | 2078篇 |
免费 | 68篇 |
国内免费 | 132篇 |
专业分类
系统科学 | 23篇 |
丛书文集 | 50篇 |
教育与普及 | 15篇 |
理论与方法论 | 4篇 |
现状及发展 | 348篇 |
研究方法 | 1篇 |
综合类 | 1833篇 |
自然研究 | 4篇 |
出版年
2024年 | 2篇 |
2023年 | 10篇 |
2022年 | 18篇 |
2021年 | 19篇 |
2020年 | 25篇 |
2019年 | 22篇 |
2018年 | 31篇 |
2017年 | 44篇 |
2016年 | 43篇 |
2015年 | 73篇 |
2014年 | 97篇 |
2013年 | 67篇 |
2012年 | 118篇 |
2011年 | 85篇 |
2010年 | 77篇 |
2009年 | 105篇 |
2008年 | 125篇 |
2007年 | 126篇 |
2006年 | 106篇 |
2005年 | 98篇 |
2004年 | 105篇 |
2003年 | 100篇 |
2002年 | 70篇 |
2001年 | 62篇 |
2000年 | 58篇 |
1999年 | 60篇 |
1998年 | 61篇 |
1997年 | 39篇 |
1996年 | 47篇 |
1995年 | 35篇 |
1994年 | 31篇 |
1993年 | 28篇 |
1992年 | 37篇 |
1991年 | 21篇 |
1990年 | 38篇 |
1989年 | 31篇 |
1988年 | 31篇 |
1987年 | 37篇 |
1986年 | 27篇 |
1985年 | 52篇 |
1984年 | 17篇 |
排序方式: 共有2278条查询结果,搜索用时 15 毫秒
11.
采用吸附式电极记录了荒漠沙蜥在不同体温条件下 ,在体心脏的单相动作电位 (MAP:Monophasic Action Potential) .实验温度为 :5℃ ,10℃ ,15℃ ,2 0℃ ,2 5℃ ,30℃和 35℃ 7个温度等级 .结果表明 :(1)在最适温度 (2 0~ 2 5℃ )时 ,荒漠沙蜥心脏单相动作电位的基本波形与高等动物及人类基本相似 ;但也有其特殊性 :动作电位 0期除极期最大幅值为 11.4 2± 2 .5 8m V;复极 2期电位幅值基本保持恒定 ,呈平台期 (PP2 :Plateau Phase2 ) ;在复极 3期末 ,电位基本恢复到 0电位(膜电位为 0 m V) .(2 )随着体温的升高 (5~ 35℃ ) ,荒漠沙蜥心脏单相动作电位时程呈温度依赖性减小 ;0期最大除极速度 (Vmax)呈温度依赖性增加 (5℃为 77.0 8± 4 7.11m V/ms;35℃为 614.63±10 5 . 0 2 m V/ms) ;低于最适温度时 (2 0~ 2 5℃ ) ,在 2相平台期末 ,又出现一次除极电位 (后隆起波 ) .其最大电位为 0 .5 8± 0 .18m V.(3)随着体温的升高 ,荒漠沙蜥心脏单相动作电位复极 1期电位呈温度依赖性降低 (5℃为 2 .2 4± 1.31m V/ms;35℃为 0 .82± 0 .4 9m V/ms) .(4 )心电图 (ECG)的同步记录表明 :心电图中的 QRS波与心脏单相动作电位 0期除极完全相对应 ;T波与动作电位的复极 3期相对应 . 相似文献
12.
在急性冷驯化阶段(1h,4h,8h),中缅树膈肌线粒体状态IV呼吸与对照比较8小时组有显著的增强,心肌线粒体状态IV呼吸与对照比较在4小时和8小时有显著增强;在持续冷驯化阶段(1d,7d,14d,28d),中缅树膈肌和心肌线粒体状态IV呼吸与对照比较均有所增强,其中28天组增强十分显著。这一系列变化对于探讨中缅树膈肌与心肌在冷驯化中质子漏产热特征具有一定的意义。 相似文献
13.
P. Levy G. Cherqui A. Robert D. Wicek J. Picard 《Cellular and molecular life sciences : CMLS》1989,45(6):588-591
Summary During the spontaneous differentiation (day 5 to day 15 of the culture) of Caco-2 cells, the sulfation of cell layer glycosaminoglycans increased, whereas protein kinase C activity was concomitantly redistributed from the membrane to the cytosol. The protein kinase C activators, 4-phorbol 12-myristate, 13-acetate and 1,2-dioctanoyl-glycerol inhibited glycosaminoglycan sulfation. By contrast, 4-phorbol 12, 13 didecanoate was ineffective.These results suggest that membrane-bound PKC may exert a modulatory effect on glycosaminoglycan sulfation, and this effect is gradually attenuated as Caco-2 cell differentiation progresses. 相似文献
14.
H. Nawrath J. Rupp H. Jakob U. Sack F. Mertzlufft W. Dick 《Cellular and molecular life sciences : CMLS》1989,45(4):337-339
Summary The opioid agonists morphine (selective for -receptors) and ethylketocyclazocine (selective for kappa-receptors), at concentrations evoking strong effects in neuronal structures, did not significantly affect the configuration of the intracellularly recorded action potential and the force of contraction in ventricular heart muscle isolated from guinea pigs, rabbits and man. These results suggest that any changes of heart functions in vivo in response to opioid-like drugs are probably not mediated postsynaptically at the myocardial cell membrane but rather presynaptically, influencing the release of noradrenaline and/or acetylcholine from the nerve terminals. 相似文献
15.
B. Lindblad W. E. Burkel T. W. Wakefield L. M. Graham J. C. Stanley 《Cellular and molecular life sciences : CMLS》1988,44(3):223-224
Summary The most important effect of dihydroergotamine is venoconstriction, but certain metabolic effects and changes in vessel prostanoid activity have also been suggested. In this study endothelial cell production of 6-keto PGF1 and TxB2 was quantitated in vitro. No evidence of altered prostanoid production was noted after incubation with dihydroergotamine (exposure ranging from 5×10–3 to 5×10–7 g/l). Similarly, no effect of dihydroergotamine on the growth rates of endothelial cells or smooth muscle cells in vitro was documented. 相似文献
16.
M. Caron G. Cherqui D. Wicek J. Capeau J. Bertrand J. Picard 《Cellular and molecular life sciences : CMLS》1988,44(1):34-37
Summary Insulin stimulation of glycogen synthesis was nearly abolished in hepatoma cells shortly treated with 4 ß-phorbol 12 \-myristate, 13 -acetate (protein kinase C activation) but remained unmodified in cells chronically treated with the phorbol ester (protein kinase C depletion). Thus, although exogenous activation of protein kinase C results in an inhibition of insulin action, protein kinase C depletion has no influence on this process. The results suggest that, in hepatoma cells, no endogenous activation of protein kinase C may occur in response to the signal triggered by insulin. 相似文献
17.
In 1986, Brown and Clemmons (Proc. natl Acad. Sci. USA83 (1986) 3321) showed that platelets contain a substance, platelet-derived growth inhibitor (PDGI), that inhibits in vitro endothelial cell replication. Although platelets are rich in transforming grwoth factor (TGF-), PDGI was considered not to be related to TGF-, on the basis of its reported properties (extraction from platelets at neutral pH, binding to heparin-Sepharose). However, we purified PDGI to near homogeneity and showed that on the basis of HPLC retention behavior, in vitro growth inhibitory activities with several cell types, receptor binding, and immunoneutralization of growth inhibitory activity with specific anti-TGF- type 1 antibodies, PDGI is most probably identical with TGF- type 1. 相似文献
18.
Summary During molt-induced atrophy of the pectoralis muscle of the Giant Canada Goose (Branta canadensis maxima), the zinc content of the muscle was significantly reduced (p0.0139), though the concentration of zinc per unit weight of muscle appeared higher (p0.0232). Zinc lost from the muscle during molt could be utilized for growth of the new flight feathers.Acknowledgments. Funds for this study were obtained from an operating grant awarded to J. C. G. by the Natural Sciences and Engineering Research Council of Canada. 相似文献
19.
J. S. Nowak 《Cellular and molecular life sciences : CMLS》1985,41(1):88-89
Summary Somatic cell hybrids between Sp2/O-Ag14 mouse myeloma cells and lymphocytes derived from BALB/c mice hyperimmunized with sheep red blood cells (SRBC) were produced. One hybrid producing IgG1 antibody to SRBC was selected, cloned twice and subsequently transferred to BALB/c mice. After a number of transfers it was found that the antibody titer in ascitec fluid gradually decreased. Cytogenetic analysis revealed gradual chromosome loss in the hybrid clone, which produced progressively less antibody. 相似文献
20.
Summary Glucose-6-phosphate dehydrogenase and N-acetyl--glucosaminidase activities were both elevated after eccentric exercise indicating that this type of exercise causes muscle damage. Muscle damage as measured by glucose-6-phosphate dehydrogenase activity in the vastus intermedius was greater and occurred later in larger rats indicating that the susceptibility to muscle damage is increased and the repair process delayed in older and larger animals. 相似文献