鼠源单抗3G1单链抗体基因的构建及序列分析

利用Rr—PCR方法，从分泌抗汉滩病毒mAb的杂交瘤细胞系3Gl中扩增出抗体VH和VL基因，通过PCR方法获得1inker—VL基因，进而利用加端PCR技术，在扩增出的单抗体VH和1inker—VL基因两端加上限制性酶切位点，分别克隆入pUCl8中并测序．3Gl VH基因长360bp，编码120个氨基酸；VL基因长324bp，编码108个氨基酸．在pUCl8载体中将VH和1inker—VL基因连接成ScFv基因．     

天然气中微量单体烃浓缩富集分析方法的研究

本文主要探讨了天然气中微量重组分及C₉,前的单体经的分析方法。在解剖了目前存在的天然气分析方法的基础上;探索出了一种简单新型浓缩方法,用于对天然气中微量重单体经的分析侧定。通过实脸,给果表明,该法足可行的。     

智能交流稳压器的设计

本文介绍了一种用ＰＩＣ１６Ｃ７１单片机控制的由直流电机驱动自耦调压器的手柄而实现的智能交流稳压器的设计思路。文中详细阐述了信号采样、电机驱动电路的工作原理和ＰＩＤ控制算法,并给出了软件流程图。     

测定金属线胀系数的一种新方法

本文给出了用激光衍射技术,测金属线胀系数的方法。这种新方法中作为传统实验的选作或校准实验。     

以MCS—51单片机为核心的恒电位防腐仪

根据防腐基本原理,向硫酸冷却器及其它化工容器或设备这些被保护体外加直流电流,维持其在溶液中的钝化状态,就能达到保护的目的．以MCS－51单片机为核心的恒电位控制系统,准确有效地测量免或分布电位和阳极电流,采用数字PID控制,保证了阳极电位恒定,具有线路简单、调试方便、可靠性高等特点．     

从单中心到双核——晚清济南城市空间结构转型研究

本文从晚清时期济南的历史背景入手,着重分析研究了清末济南自开商埠后城市空间功能的变化和空间结构的转型,即由单中心向“老城区＋商埠区”双核结构的转变,同时探讨了这种转变带来的深远影响。     

单片机控制的数据采集系统

本文论述了由8031单片机实现远程数据采集和监测功能的系统.该系统采用双CPU控制,远端CPU提供8路数据采集,本地CPU控制远端CPU,并进行数据处理、数据显示、键盘输入和系统报警等工作.通过单片机通讯的方式实现数据与控制指令的远距离传输.     

Palpal loss,single palp copulation and obligatory mate consumption in Tidarren cuneolatum (Tullgren, 1910) (Araneae,Theridiidae)

Males of Tidarren cuneolatum (Tullgren, 1910) amputate one of their palps a few hours after the penultimate moult, like T. sisyphoides (Walckenaer, 1841) from the USA (Branch, 1942). Hence adult males, which are minute, have only one palp, either left or right randomly. This palpal organ is not oversized, when compared with other small spiders. During courtship females are unusually active, signalling receptivity by continuous twanging with legs II. Males construct a mating thread. Copulation involves one insertion, which lasts ca 4 min. Thus, only one receptaculum is inseminated during copulation. With the advance of insertion the male's prosoma becomes shrunken. Copulation regularly ends in mate consumption. In copulation with a virgin female the palp was inserted contralaterally. Females taken in the field had both receptacula filled with sperm and therefore were polyandrous. Re-mating was also observed in the laboratory. Remarkably the second male performed an ipsilateral insertion, if it possessed the same palp as the previous male. Probably the virgin receptaculum was recognized. Postembryonic development is rapid in males, which moult three times and mature ca 41 days after hatching from the cocoon. Females need four or five moults and ca 69 days to reach maturity and then survive ca 2-4 months.     

CRF:A Scheduling of Multi-Granularity Locks in Object-Oriented Database Systems

1.INTRODUCTIONRecelltly,object-orienteddatabasesystem(OODB)hasbecomepopular,becausemailyadvanceddata-intensiveapplicationssuchasCAD/CAMandmultimediadatabaseshaveemployedthistechniqlle.Manypapershavediscussedaboutwhattheobject-orientedmeansandwhatanobject-orienteddatabaseisinreference[l~4].Anewtransactionmodelforobject-orienteddatabasesystemwasproposedinreference[sl.Itisclearthatobject-orienteddatabaseshavegreatpotentialtobeusedwidelybecauseoftheirobject-orienteddesignmodelandflexibled…     

轨检仪单轨激光发射装置机械结构设计

轨检仪是铁路钢轨参数检测的主要工具之一,对轨检仪的机械结构简化创新设计十分重要,即可以提高轨检仪发射装置的便携性,又降低了劳动强度。本研究对激光发射装置机械结构移动和固定进行了创新设计,给出了具体的设计方案和部分结构图以及参数。