褪黑素的生物学功能研究进展

综述了褪黑素在调节动物生物钟，抑制性成熟，调节中枢神经系统和内分泌系统，保护消化道，促进睡眠和毛皮生长等的生物学功能及应用。褪黑素还具有抗氧化，抗衰老和抗癌症的作用。     

褪黑激素的发现及其应用

本文论述了褪黑激素的发现和它对毛皮动物冬皮成熟,生理,生殖的影响,以及在人类疾病防治与治疗方面的应用,展望了褪黑激素的应用前景。     

褪黑素单克隆抗体的制备及鉴定

为了获得抗褪黑素(MT)高效价的单克隆抗体,用甲醛将MT连结到BSA上,然后用连结物背部皮下多点免疫Balb／c小鼠,用PEG诱导免疫鼠脾细胞与Sp2／0细胞融合,经ELISA法筛选阳性克隆株及测定效价,用抗体与同类物之间的交叉反应进行特异性鉴定．最后获得5株(4C9D7、3E12C7H11E7、3E12A9D7、6A6A3A2C3、1E1E2H2H6)能稳定分泌高效价抗MT的单克隆抗体(McAb)的杂交瘤细胞。     

化学发光成像分析法检测健康食品中的褪黑激素

基于在碱性介质中,褪黑激素对H2O2与MoO42-反应产生的微弱化学发光信号有较强的增敏作用,利用高灵敏度、高分辨率的成像分析仪检测发光信号,建立了一种简单、高通量的检测褪黑激素的分析方法并将该方法用于健康食品脑白金中褪黑激素含量的测定。     

Presence in and effects of pineal indoleamines at very low level of phylogeny

The unicellular organism Tetrahymena contains serotonin and is able to take up the hormone from its mileiu. The serotonin content of the cell changes as a function of the presence of foreign exogenous hormones. This indicates a possible role of serotonin as a chemical mediator. Exogenous serotonin stimulates the RNA synthesis of Tetrahymena, and it was the only one among the hormones studied which kept the RNA level durably high. Serotonin stimulates phagocytosis and growth of Tetrahymena, and its precursors also stimulate growth. Serotonin can imprint Tetrahymena, and as a consequence of this the effect of the hormone increases in the case of further encounters. Treatment with serotonin-related molecules soon after imprinting can reduce the effect of imprinting. Melatonin can contract the pigment cells of Planaria; however, its precursors serotonin and tryptamine can do this more intensely. Both melatonin and serotonin can influenc the regeneration of Planaria, with effects which differ when different phenomena are studied. Evolutionary theories are discussed.     

褪黑激素对小鼠免疫功能的影响

采用３Ｈ－ＴｄＲ掺入法和溶血素测定法观察褪黑激素（Ｍｔ）对刀豆素（ＣｏｎＡ）诱导小鼠脾淋巴细胞增殖和半数溶血值（ＨＣ５０）的影响。结果表明，Ｍｔ于光周期傍晚腹腔注射０．０１、０．０５、０．１ｍｇ／ｋｇ能明显促进ＣｏｎＡ诱导的小鼠脾淋巴细胞增殖，提高可的松抑制的脾淋巴细胞增殖及环磷酰胺抑制的半数溶血值。提示Ｍｔ具有免疫增强作用和对抗应激引起的免疫抑制作用，在临床医学上具有广阔应用前景。     

The pineal and melatonin: Regulators of circadian function in lower vertebrates

Summary The pineal has been identified as a major circadian pacemaker within the circadian system of a number of lower vertebrates although other pacemaking sites have been implicated as well. The rhythmic synthesis and secretion of the pineal hormone, melatonin, is suggested as the mechanism by which the pineal controls circadian oscillators located elsewhere. Both light and temperature cycles can entrain the pineal melatonin rhythm. The pineal, therefore, acts as a photo and thermoendocrine transducer which functions to synchronize internal cycle with cycles in the environment. A model is presented which portrays the pineal as a major component of a multioscillator circadian system and which suggests how these multiple circadian clocks are coupled to each other and to cycles of light and temperature in the external world.     

Increased melatonin levels after hemorrhagic shock in male and female C3H/HeN mice

Although hemorrhagic shock leads to significant alterations of several hormones, e.g. ACTH, corticosterone and -endorphin, it is not known whether plasma melatonin levels are affected under this condition and if so, whether the effects are comparable in males and females. Using a radioimmunoassay, it was found that plasma melatonin levels were significantly increased in male and proestrus female C3H/HeN mice immediately after hemorrhagic shock. However, in male mice, by two hours after hemorrhage and resuscitation, plasma melatonin returned to levels comparable to those seen in control and sham-operated animals. Proestrus female mice, on the other hand, showed significantly increased plasma melatonin levels at two hours after surgery when compared to unoperated control animals. Although the significance and biological role of the transient increased plasma melatonin levels after hemorrhagic shock remain to be determined, it appears that the pineal gland and/or an extrapineal source of melatonin, of both male and proestrus female mice responds to severe hypotension by increased release of melatonin.     

线性扫描伏安法测定胶囊中褪黑素的含量

用线性扫描伏安法测定褪黑素,在2.0×10-10～1.0×10-6 mol/L浓度范围内,褪黑素氧化峰电流强度与其浓度呈良好线性关系.将该法用于检测脑白金胶囊中褪黑素的含量,得到了令人满意的结果.     

饥饿对黄鳝性腺发育及血清褪黑激素水平的影响

研究了饥饿对黄鳝对性腺发育的影响,并试图与血清中褪黑激素水平的变化相联系,实验结果表明,６周饥饿促进黄鳝性转变,６周和１周饥饿皆导致血清褪黑激素水平的午夜值明显高对照组（Ｐ〈０．０５和Ｐ〈０．００１）,由此推论,饥饿促进黄鲜性转变可能是通过提高血清褪黑激素水平实现的。