利用图像数据的相关性实现图像抗信元丢失编码

提出了ＡＴＭ（异步转移模式）环境下图像抗信元丢失编码的信息分散原则。根据这一原则提出了图像的相关分解和相关补偿技术。通过相关性分解为信元丢失的补偿提供了最相关的数据,通过最相关补偿获得最佳的补偿效果。     

Dynamic analysis of DNA damage induced miRNAs in colon cancer cells

It is known that microRNAs （miRNAs） expression profile shows substantial changes in cells under DNA damage. Here, we did miRNA microarray and quantitative real-time PCR to comprehensively identify the differentially expressed miRNAs in colon cancer cell lines HCT116 p53＋/＋ and HCT116 p53-/-. Cluster analysis revealed a panel of differentially expressed miRNAs which are regulated by p53 and/or UV-C induced DNA damage. These altered miRNAs tend to be located in chromosomes 13, X and 17. Moreover, pathways enrichment analysis estimated that MAPK pathway, focal adheren pathway, p53 pathway and Wnt pathway were mediated by these miRNAs to exert their functions in DNA damage response. Additionally, we found that miR- 320a, one of the UV-C induced miRNAs, play a role in protecting cells from DNA damage. Taken together, our results show that miRNAs are dynamic regulated in p53- dependent or -independent manners in different cell contexts and different situations following DNA damage.     

常利率复合二项风险模型的破产概率分布

研究了含利率因素的复合二项双险种风险模型的破产问题,获得了该模型下破产概率和生存概率的递推式以及所满足的积分方程.     

小鼠金属硫蛋白基因-I 在CHO细胞中稳定表达及其在医疗方面的应用

将小鼠的MT-I基因插入pSV₂-dhfr质粒的EcoRI位点,构建了具有SV₄₀和MT双启动子并正向插入MT-I基因的表达载体。用改良的磷酸钙/DNA沉淀法将表达载体导入CHO-dhfr-细胞内,用不含次黄嘌呤(H)和胸腺嘧啶(T) 的选择培养基筛选出稳定表达MT阳性克隆D-22,经检测10⁶细胞的 MT 表达量约为1.8 μg,D-22细胞对Cd²⁺浓度抗性较之CHO-dhfr-细胞高14倍。为了研究金属硫蛋白对正常细胞所具有的保护作用,将不同浓度的顺铂分别处理无MT表达的CHO-dhfr-细胞和有MT表达的D-22细胞。实验发现顺铂对D-22细胞和CHO-dhfr^-细胞 50%生长抑制浓度(IC50)分别是0.145μmol/L 和0.04μmol/L。上述研究表明MT对正常细胞有一定的保护作用,因而通过诱导正常细胞提高MT的表达量,同时使用MT阻断剂阻断肿瘤组织的MT合成,这样可能会为治疗某些顽固肿瘤开辟一条新途径。     

Mad2蛋白在有HeLa细胞有丝分裂期的动态定位

准确的染色体分离依赖于有丝分裂过程的精确调控,包括有丝分裂的时间,及纺锤体检查点的正确调控等。通过动态观察有丝分裂染色体的运动可对上述研究进行精确定量。结果显示,我们利用逆转录病毒系统成功构建了稳定融合表达绿色荧光蛋白GFP-H2B的HeLa细胞系,结合细胞同步化方法,建立了一套利用活细胞荧光共聚焦显微镜观察HeLa细胞有丝分裂的实验体系。     

Rapid reversion of aging phenotypes by nicotinamide through possible modulation of histone acetylation

Aging appears to be an irreversible process. Here we report that nicotinamide (NAA) can induce rapid and reversible reversion of aging phenotypes in human diploid fibroblasts in terms of cell morphology and senescence-associated β-galactosidase activity. Although NAA seems to enhance the replicative potential of the cells, it has little effect on their growth rate and life span, suggesting that NAA action is rather separated from the cellular replicative system. The effects are unique to NAA: none of the NAA-related compounds examined (an NAD precursor/niacin, NAD analogs, and poly(ADP-ribose) polymerase inhibitors) exerted similar effects. Thus, NAD-related metabolism and poly(ADP-ribosyl)ation are unlikely related to the NAA action. On the other hand, histone acetyltransferase (HAT) activity was elevated in NAA-exposed cells, while in aged cells, HAT activity and histone H4 acetylation were lowered. Taken together, the results suggest that NAA may cause rejuvenation by restoring, at least in part, altered gene expression in aged cells through its activation of HAT. Received 27 August 2001; received after revision 15 October 2001; accepted 15 October 2001     

风险相依的双险种模型下的生存概率

将双险种poisson风险模型推广到一种相依的结构,即某险种的索赔到达间隔分布依赖于前一时刻另一险种的索赔大小. 利用拉普拉斯变换,推导出了其生存概率的表达式.     

国家非物质文化遗产的旅游化生存模式研究——以国家级“非遗”常州梳篦为例

结合国家级＂非遗＂常州梳篦个案,对非物质文化遗产的保护和传承模式进行研究,探讨了非物质文化遗产的＂旅游化＂传承保护模式,为非物质文化遗产保护和传承提供参考。     

文化转型中的婚姻焦虑——对冯小刚电影《手机》的评析

冯小刚的电影《手机》,主要讲述在社会转型、文化转型的时代背景下,迅猛发展的当代技术社会对中国传统婚姻生活的冲击和影响.婚姻双方对此表现出多重焦虑:对时空、环境和情感的错位焦虑,对历史和个体成长的焦虑,以及女性解放潮流下的性别焦虑.