不同月龄实验大鼠造血细胞的定量观察

目的观察老年大鼠与青年大鼠造血功能的差别。方法采用含有15％刺激因子（GM-CSF）的大鼠肺条件培养基进行单层软琼脂法培养大鼠骨髓造血祖细胞，进行定量观察。结果肺条件培养基内刺激因子（GM-CSF）浓度为15％时活性最好。2．5月龄大鼠骨髓，每4×10^5个有核细胞中形成细胞集落（GM-CFU）为92．5±23．8，而24月龄老年大鼠则为23．1±9．6。以2．5月龄青年大鼠GM-CFU为100％，12月龄大鼠为37％，18月龄大鼠为35．7％，24龄大鼠则为27．2％。结论骨髓造血功能随月龄的增加逐渐减弱，老龄大鼠骨髓造血功能明显下降。     

股骨近端骨密度结合其几何参数在老年人骨质疏松性髋部骨折诊治中的临床意义

目的探讨股骨近端骨密度与几何结构参数在老年人骨质疏松性髋部骨折诊治中的临床意义。方法将65例髋部骨折患者作为实验组。同期健康体检的60例老年志愿者为对照组。在骨盆片上测量股骨近端几何参数,在股骨颈、Ward’S三角和转子处测量BMD值（髋部骨折组对其健侧进行检测）,对结果进行比较分析。结果实验组的BMD值低于对照组（P〈0．05）;股骨颈骨皮质厚度与股骨颈BMD值有相关性（r=0．54,P〈0．05）;股骨距内侧皮质厚度、转子处BMD值、颈干角和Ward’s三角BMD值相结合在预测髋部骨折中价值较大（r=0．78,r2=0．57,P〈0．05）。结论股骨近端骨密度结合其几何结构参数在骨质疏松性髋部骨折的诊治中具有较大的临床意义。     

骨髓核素显像对再生障碍性贫血临床诊断的评价

目的:评价骨髓核素显像对再生障碍性贫血(简称再障)的临床诊断价值。方法:进行全身骨髓显像的血液病患者共63例,分为再障组(n=24例)和非再障组(n=39例),以临床表现、血象、骨髓涂片和(或)骨髓活检结果为诊断标准,评价骨髓显像对再障诊断的灵敏度和特异性。结果:全身骨髓显像结果以骨髓活性2级作为正常;再障诊断的灵敏度为83 3%(其中重型再障90 0%,慢性再障78 6%),特异性为61 5%。结论:全身骨髓核素显像是一种无创、安全、灵敏的检查方法,对了解再障的全身功能性骨髓分布及各部位骨髓造血功能有独到优势。     

白血病细胞的体外净化原理与方法

介绍白血病治疗的主要方法和基本原理，并对其进行比较，重点介绍光动力作用体外净化白血病细胞的原理和影响净化效果的基本因素及其研究发展趋势。     

WKA大鼠骨髓细胞的观察

在肿瘤的治疗研究中，化疗为不少患者解除病痛，延长生命，但与此同时，化疗药物也极大的损伤了人类造血系统，破坏了人体的正常免疫功能，各种免疫增强剂的应用，改善了患者的机体状态，但对其疗效的观察，包括对患者骨髓细胞的分析，探讨其毒性大小，大鼠做为人类的替难者，在肿瘤的研究中首当其冲，为此，我们对我所饲养的ＷＫＡ大鼠骨髓细胞进行了分析观察，为应用大鼠作实验提供依据。     

P-选择素/P-选择素糖蛋白配体-1与骨髓祖细胞胸腺归巢

骨髓衍生的祖细胞归巢入胸腺是T细胞发育必需的过程,因为胸腺不包含具有自我更新能力的造血干细胞,它需要从血液中添补祖细胞,此过程依赖多级细胞粘附分子和趋化因子的衔接.细胞粘附分子选择素和它的配体PSGL-1在淋巴样祖细胞归巢入胸腺的过程中发挥重要作用.综述了选择素/配体PSGL-1的生物学特征及其相互作用对骨髓祖细胞胸腺归巢及胸腺-骨髓反馈环的影响和作用机制.     

吸入布地奈德对哮喘儿童肺功能的改善及安全性评价

目的探讨雾化吸入布地奈德对哮喘儿童肺功能的改善及安全性。方法选择95例6．48月的哮喘患儿持续（6月）吸入布地奈德,每日250μg,2次／d,治疗前及治疗6月后分别测定各项肺功能指标、血皮质醇及骨密度。结果治疗6月后,肺功能有明显改善,血皮质醇及骨密度无明显变化。结论吸入布地奈德能改善哮喘患儿肺功能,降低气道高反应性,对患儿肾上皮质功能及骨密度未见显著影响。     

Fabrication and biological evaluation of polyether ether ketone(PEEK)/bioceramic composites

The repair of vascularized bone defects represents a significantly clinical challenge, and vascular regeneration is one of the necessary factors to promote bone tissue regeneration. To effectively repair large bone defects, new bone tissue must regenerate with a rich vascular network. Therefore, the development of biomaterials that can promote the regeneration of vascularized bone tissue is currently receiving attention from researchers. In this study, Li–Ca–Si bioceramics (LCS) containing Li, Ca, and Si elements was developed, then LCS was compounded with PEEK to prepare PEEK+10% LCS, PEEK+20% LCS, PEEK+30% LCS, and the effect of LCS-PEEK composite biomaterials on the proliferation and angiogenic ability of human umbilical vascular endothelial cells (HUVECs) further explored by Cell Counting Kit-8 (CCK-8), scanning electron microscope (SEM), quantitative real-time PCR (QPCR), Western Blotting and enzyme linked immunosorbent assay (ELISA). The results showed that HUVECs inoculated on 30%LCS ?+ ?PEEK material exhibited the best proliferation ability. And the adhesion ability of endothelial cells on PEEK gradually increased with the increase of LCS contents. Furthermore, the angiogenic ability of HUVECs on LCS-PEEK composites was examined using QPCR and Western blotting, and the results showed that the expression of angiogenic-related genes and proteins of HUVECs on PEEK composites gradually increased with increasing LCS concentration. These results demonstrated that the angiogenic ability of HUVECs was effectively stimulated by LCS-modified PEEK materials. The present results indicate that the PEEK material can be modified with bioceramics to promote angiogenesis, and this study lay the foundation for the subsequent development of scaffolds that promote vascularized bone tissue regeneration.     

Effect of Spatholobus suberectus Dunn on proliferation of progenitor cells in mice with bone marrow depression

AIM： To study the effect of Spatholobus suberectus Dunn on the prolilferation and hematonic mechanism of Spatholobus suberectus Dunn. Methods： The techniques of culture of hematopoietic cell and hematopoietic growth factor （HGF） assay were used. The method of semi-solid culture with methylcellulose of CFU-GM, CFU-E, BFU-E, CFU-Meg was adopted in bone marrow depressed mice which treated with Spatholobus suberectus Dunn for a long time. Results： Spatholobus suberectus Dunn could obviously promote the proliferation of bone morrow cells and spleen lymphocytes in healthy and anaemic mice. The cuhure medium of spleen cell, macrophage, lung and skeletal muscle treated with Spatholobus suberectus Dunn had much stronger stimulating effects on hematopoietic cells. The numbers of CFU-GM, CFU-E, BFU-E, CFU-Meg in bone marrow depressed mice were raised distinctly under the control of Spatholobus suberectus Dunn as compared with those of contrast group. Conclusions： Spatholobus suberectus Dunn may enhance hematopoiesis by stimulating directly and/or indirectly stroma cell in hematopoietic inductive microenvironment and muscle tissue to secrete some HGF （Epo, GM-CSF, IL, and MK-CSF）. It can promote the proliferation and differentiation of hematopoietic cells in anaemic mice. This is one of the biological mechanisms for hematonic effect of Spatholobus suberectus Dunn.