Therapeutic induction of angiogenesis by direct myocardial administration of an adenovirus vector encoding human hepatocyte growth factor gene and its safety

After the study in vitro and in rats, we assessed further the effects and safety of local angiogen therapy using intramyocardial delivery of an adenovirus carrying hepatocyte growth factor gene (Ad-HGF) in a canine ischemia model. The angiogenic activity of Ad-HGF was evaluated from three aspects. First, the augmentation of collateral vessel development was assessed by angiography 30 d after surgery. The results showed that the density of collateral vessels in treated group was higher than that of control group. Secondly, infarct size was evaluated by TTC staining and image analysis. The results showed that the infarct size of treated group was smaller than that of control group. Thirdly, the myocardial regional blood flow was determined by the method of colored microspheres. The results showed that the blood flow recovered to the level before ligation in treated group, but that of the control group was lower than normal level. In addition, during the study of chronic toxicity, we tested the anti-adenovirus antibodies by neutralization method. The antibodies yielded after the fourth injection decreased slowly from peak level and disappeared 12 weeks after drug withdrawal. Overall, Ad-HGF can promote angiogenesis in ischemic myocardium and reduce infarct size. So this method may be considered as a therapeutic angiogenesis induction strategy for ischemic disease including myocardial infarction and peripheral artery disease. At the same time, Ad-HGF could induce the yield of anti-adenovirus antibodies to neutralize adenovirus, which may be the mechanism of adenovirus clearance.     

2型糖尿病视网膜病变大鼠模型建立

目的 建立2型糖尿病视网膜病变大鼠模型.方法 80只雄性SD大鼠随机分成两组:空白对照组(普通饲料)及T2DM组(高脂高糖饲料喂养4周联合30 mg/kg链脲佐菌素腹腔注射).链脲佐菌素注射后观察大鼠一般情况、每周测量体质量、随机血糖、空腹血糖、空腹血清胰岛素等指标,计算并分析胰岛抵抗指数(HOMA-IR).分别在血糖...     

静脉滴注法CT胆系造影对阻塞性胆管病变的诊断价值

【目的】研究静脉滴注法CT胆系造影对胆道梗阻性病变的临床应用价值。【方法】对18例怀疑胆道梗阻的患者进行静脉滴注法CT胆系造影。螺旋CT扫描从膈顶至胰腺钩突区,以MIP、VR、MPR和CPR合成三维胆管树影像。【结果】18例静脉滴注法CT胆系造影均显示了胆管梗阻的部位、原因、程度及范围,胆管解剖和异常在MPR、CPR和MIP图像比轴位图像显示清楚。【结论】静脉滴注法CT胆系造影,对梗阻的判断准确可靠,可提供高质量的围手术期胆系三维影像。     

多层螺旋CT研究:高血压病及糖尿病对冠状动脉起始部的影响

目的:利用多层螺旋CT研究高血压病及糖尿病对冠状动脉起始部管腔解剖径线的影响。方法:采用病例—对照研究,收集自2005年9月-2010年12月间,在新疆友谊医院接受多层螺旋CT冠状动脉CTA检查的受检者263例,年龄35-65岁,将其分为3组。正常成年人组n=94、高血压病组n=85、高血压病并糖尿病组n=84。利用工作站的冠状动脉分析软件对检查所得图像进行重建和冠状动脉起始部管腔的解剖径线的测量。结果:左、右冠脉最大内径及腔面积:正常成年人组、高血压组、高血压病并糖尿病组之间三组之间的差异均无统计学意义(P>0.05)。结论:高血压病和糖尿病不易引起左右冠状动脉起始部(距主动脉窦6-7mm处)管腔解剖径线发生改变。     

Off-pump coronary artery bypass grafting versus on-pump coronary artery bypass grafting： which is better in patients with chronic obstructive pulmonary disease？

To evaluate the clinic outcome of off-pump coronary bypass grafting (OPCABG) of patients with coronary heart disease and chronic obstructive pulmonary disease, we collected and analyzed 1998-2002 data on 28 patients with these two diseases who had received off-pump coronary bypass operation in our hospital, and compared with data on those who also had the same two diseases but received on-pump coronary artery bypass at same time. There were no operation-related death;one died of respiratory failure 14 days after operation while staying in hospital; there were more respiratory complications in the conventional coronary artery bypass grafting group (CCABG) than in the OPCABG group; and the PaO2/FiO2 in the CCABG group was higher than that in the OPCABG group during operation because of CPB, but lower than that in the OPCABG group 6-12 hours after operation. OPCABG seemed more suitable than CCABG for coronary artery disease patients with chronic obstructive pulmonary disease due to less damage to their oxygen-exchange capability and the fewer respiratory complications.     

逆行胰胆管造影术对阻塞性黄疸的诊断价值

1临床资料 1．1一般资料 24例黄疸患者，男16例，女8例，年龄23-73岁，平均50岁。检查前全部均有黄疸，伴腹痛13例，伴发热5例。受检前全部经B超或彩超检查，结果为：胆管结石并扩张11例，胆囊炎并结石5例，胆囊癌1例，胆总管梗阻3例，胆总管囊肿并结石3例，肝内胆管结石1例。     

改进T管胆道造影方法研究

目的探讨改良传统的T管胆道造影方法。方法使用2种加入地塞米松和利多卡因的不同浓度的造影剂，取不同体位给患者造影，另取一传统方法造影组对照。结果改良型T管道造影显像优秀率100％，术中患者不适率6％，诊断符合率87％；传统法T管造影显像优秀率65％，术中患难与共者不适率35％，诊断符合率60％。结论改良型T管造影可明显提高显像优秀率、诊断符合率，降低术中患者不适率。     

基于Hessian矩阵的冠状动脉中心线的跟踪算法

冠状动脉血管造影对医生临床诊断心血管疾病非常有帮助。在冠状动脉造影图像上对冠脉中心线的正确提取是冠脉边缘定位和三维重建的基础。该文提出的中心线提取方法是对经典Sun算法的改进,此方法结合了Hessian矩阵特征向量和Canny算子来进行准确提取。实验结果表明:结合Hessian矩阵特征向量的方向,较好地解决了冠脉造影图像中冠脉曲率变化剧烈而跟踪不准确的情况;结合Canny算子的半径计算,较好地解决了冠脉造影图像中冠脉重叠和交叉出现时错误跟踪的情况。因此,与经典Sun算法相比,本文提出的方法有很好的鲁棒性和准确性。