原发性胃淋巴瘤的16层CT诊断

目的探讨原发性胃淋巴瘤的16层田表现特征及诊断价值。方法回顾性分析18例经病理证实的原发性胃淋巴瘤的16层CT表现,包括病变部位、胃壁及粘膜改变。结果18例均为B细胞起源非霍奇金淋巴瘤。肿瘤呈弥漫浸润型11例,肿块型5例和节段溃疡型2例。16层CT平扫示肿瘤密度均匀14例,不均匀4例,16层CT增强扫描示病灶呈轻、中度均质增强16例,溃疡形成后增强不均匀2例。结论原发性胃淋巴瘤的16层CT表现特征对诊断和鉴别诊断有一定价值。     

非何杰金氏病淋巴瘤的中国和澳大利亚患者间的免疫组织化学比较研究(英文)

本文对22例非何杰金氏病淋巴瘤(NHL)的中国患者和25例澳大利亚患者进行免疫组织化学的比较研究。两组患者的淋巴瘤组织切片均按美国国立癌症研究所1983年的工作分类方案作了病理分型,并以免疫过氧化酶物法进行比较形态学的观察。中国组内的瀰漫型、混合细胞性(小裂核细胞和大细胞)和组织细胞性NHL,较澳大利亚组为多;瘤细胞的浆细胞样分化和瘤组织间质内的嗜酸性白细胞浸润亦然。细胞浆内免疫球蛋白的检出,中国组(尤以具有浆细胞样分化者)较澳大利亚组为低。瘤细胞浆内免疫球蛋白的重链,中国组以γ为主,澳大利亚组则属μ和α。轻链的分配在中国组内无明显差别,在澳大利亚组则以λ居多。作者并探讨了上述差异的意义。     

Endogenous copper is cytotoxic to a lymphoma in primary culture which requires thiols for growth

Summary With the use of bathocuproine sulfonate, a copper-specific chelator as an indicator, we have demonstrated that copper ions, present as a natural medium constituent are toxic to the growth of a lymphoma in primary culture and are principally responsible for the growth requirement of mercaptoethanol and other thiols. By chelating trace copper normally present in the medium, bathocuproine sulfonate retarded the oxidation of cysteine to poorly utilized cystine, thus permitting its direct utilization by the cells for growth.     

含马兜铃酸中药单方马兜铃及复方龙胆泻肝丸的体外遗传毒性研究

采用Ames试验和小鼠淋巴瘤致突变试验(MLA)来检测单方马兜铃及复方龙胆泻肝丸的遗传毒性,评价其相关细胞毒性和复方减毒效果;为进一步建立综合的中药遗传毒性测试平台提供试验依据.分别通过对含马兜铃酸(Aristolochic Acid,AA)浓度为20和40μg/mL,加S9或不加S9的条件下的两味中药的Ames法检测;以及采用MLA96孔微孔板接种法分别对单复方含马兜铃酸浓度为5μg/mL的L5178Y/tk /--3.7.2c细胞进行染毒,并进行接种效率(PE),相对总增长率(RTG)和突变频率(MF)的测定.结果表明单方马兜铃具有细胞毒性且致突变性较强,以诱导大范围DNA损伤为主;而复方龙胆泻肝丸具有较明显的减毒效果;MLA和Ames试验组合适用于体外中药遗传毒理检测.     

A feature ensemble technology to identify molecular mechanisms for distinction between multiple subtypes of lymphoma

Due to complexities and genetic heterogeneities of biological phenotypes, robust computational approaches are desirable to achieve high generalization performance with multiple classifiers, perturbations of the data structures, and biological interpretations. The purpose of this study is to extend our developed ensemble decision approach to distinguish multiple heterogeneous phenotypes and to elucidate the underlying molecular bridges that intertwine the subtypes. Our work identifies the significant molecular mechanisms （disease-relevant genes and functions） that underpin the complex molecular mechanisms for distinction between multiple phenotypes. Feature genes and hierarchical gene cores identified by our method have achieved high accuracy in the classification of multiple phenotypes. The results show that the proposed analysis strategy is feasible and powerful in the classification of biological subtypes and in the explanation of the molecular connections between clinical phenotypes. Biological interpretations with Gene Ontology revealed concerted genetic pathways for some lymphoma subtypes.     

裸鼠自发性肿瘤病理学及免疫组织化学研究

研究了１９８１￣１９９５年进行人类肿瘤异位移植的２８５０只ＢＡＬＢ／ｃ裸鼠,发现４例裸鼠自发瘤,发病率为０．１４％。病理学及免疫组化证实其中３例为Ｂ细胞生恶性淋巴瘤,病变主要累及全身淋巴结、脾脏、肝、肾和肺;１例为肛管高分化然状细胞瘤。该４例裸鼠所移植的人类肿瘤均未生长。本文讨论了裸鼠自发瘤的发病率、病理学类型和发生原因。