关于医学院校体育教学理念改革的几点思考

在体育教学中教师占有重要地位，是体育教学改革的组织和执行。因此。体育教师是否具有改革所需要的新观念、新理论，进而指导自己的教学活动，执行教改方案，是改革成功与否的重要因素。本就医学院校体育教学理念改革的问题谈几点看法，供大家参考。     

健康教育在护理工作中的应用

目的：帮助病人了解自身疾病控制的有效方法，建立一套有效的预防、治疗、保健措施．方法：对护士健康教育技巧进行深入探讨．结果：病人对护理工作满意度显著增高．结论：针对病人，病种，通过合理的宣教，拉近护患之间的距离，增加护患沟通，了解患者的需求和存在问题，从中得到及时解决，为促进患者的康复起到重要作用．     

电子商务背景下的保险产业组织演进

本文从产业组织理论角度 ,分析了电子商务这一现代复合商业技术 ,研究它正在对我国保险产业演进的深远影响 :首先 ,电子商务对保险企业的行为影响表现在业务内容和方式的改变、提升企业管理水平、使企业组织结构更加扁平化、以及产品定价等方面 ;其次 ,电子商务对保险产业的资源配置效率、中小企业竞争力、保险业务范围和市场空间等产生深刻影响 ,从而影响了整个保险产业的经济绩效 ;最后 ,通过分析指出 ,电子商务正在加速我国保险产业向垄断竞争的演进趋势     

MGB probe assay for rapid detection of mtDNAl1778 mutation in the Chinese LHON patients by real-time PCR

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.     

Keratinase production and keratin degradation by a mutant strain of Bacillus subtilis

A new feather-degrading bacterium was isolated from a local feather waste site and identified as Bacillus subtilis based on morphological, physiochemical, and phylogenetic characteristics. Screening for mutants with elevated keratinolytic activity using N-methyl-N＇-nitro-N-nitrosoguanidine mutagenesis resulted in a mutant strain KD-N2 producing keratinolytic activity about 2.5 times that of the wild-type strain. The mutant strain produced inducible keratinase in different substrates of feathers, hair, wool and silk under submerged cultivation. Scanning electron microscopy studies showed the degradation of feathers, hair and silk by the keratinase. The optimal conditions for keratinase production include initial pH of 7.5, inoculum size of 2% （v/v）, age of inoculum of 16 h, and cultivation at 23 ℃. The maximum keratinolytic activity of KD-N2 was achieved after 30 h. Essential amino acids like threonine, valine, methionine as well as ammonia were produced when feathers were used as substrates. Strain KD-N2, therefore, shows great promise of finding potential applications in keratin hydrolysis and keratinase production.     

刍议剧烈运动对人体健康的不良影响

通过运动与健康的研究,着重探讨了剧烈的体育运动对人体健康的不良影响。提出机体的健康有赖于适度的体育运动,更有赖于大脑的运动的观点。     

组织的柔性管理研究

组织的柔性对其生存发展起着至关重要的作用,组织的柔性高适应环境变化的能力就强,就能在瞬息万变的市场竞争中获胜,使组织能够持续发展和壮大。这就要求各种组织努力提高管理的柔性,来适应环境变化,提高管理效率。本文主要研究柔性管理对组织的作用、表现形式以及柔性管理的实施要注意的问题。     

《世说新语新校》中的笺疏商兑

《世说新语》一书，已传世较久，舛误疑点不少，校释工作难以做到尽善尽美。先哲与时贤在词语的校勘考释方面做了不少工作，成绩斐然。李天华先生所撰《世说新语新校》一书就颇有创获，但书中仍有若干笺疏，值得进行商兑。     

从未改变的灵魂——《在人间》中作者的心灵体现

高尔基在其自传体三部曲中记录了他充满磨难的成长经历,也展现了一个革命者淳朴而坚韧的灵魂。《在人间》中阿廖沙的爱心、抗争、探索、选择及建设性的人生态度,体现着作者宽广的精神世界,这对身处现代和后现代文化语境中生存的人们具有十分重要的精神价值。     

冶金级硅酸洗提纯研究

冶金工艺被认为是非常有前途的一种有别于传统的西门子工艺而且能够满足市场对太阳能级硅需求的工艺.冶金级硅的预处理是冶金提纯工艺路径中非常重要的一个环节,因为酸洗可以有效地去除硅中的金属杂质例如铝、铁和钙等.通过系统性的对比试验对酸洗工艺去除硅中杂质的效果进行了详细的研究,分别探讨了酸的种类、酸的浓度、酸洗时间、酸洗温度以及硅粉颗粒粒径对杂质去除效率的影响.研究结果表明最佳的酸洗工艺参数为15%的盐酸,80℃,10h和100μm.在最佳酸洗工艺参数条件下金属杂质铝、铁和钙的去除率分别为70.90%、94.82%和82.69%.