新生大鼠海马神经干细胞的体外培养及鉴定

的探讨新生大鼠海马神经干细胞（NSC）的体外培养和诱导分化的条件和特点。方法分离出生1d大鼠海马,在表皮生长因子、碱性成纤维生长因子和B27联合作用下使其稳定增殖,用5-溴脱氧尿苷（BMU）标记处于增殖状态的神经干细胞,应用免疫荧光染色方法行巢蛋白（Nestin）、5-溴脱氧尿苷（BrdU）、β-Ⅲ型微管蛋白（Tuj-1）、波形蛋白（Vimenfin）和Galc-C免疫荧光染色,对NSC的增殖及其分化的细胞进行鉴定。结果体外培养的NSC增殖成神经干细胞球并传代,鉴定为Nestin染色阳性细胞和5-溴脱氧尿苷（BrdU）标记染色阳性细胞,并可诱导分化为神经元细胞（Tuj-1染色阳性细胞）、神经胶质细胞（Vimentin染色阳性细胞）和少突胶质细胞（GMc-C染色阳性细胞）。结论采用无血清培养基中加入特定生长因子的培养技术,可培养出在体外稳定增殖并有多向分化潜能的新生大鼠海马神经干细胞。     

火炬松树干液流的研究

采用ICT-2000TE树干液流测定系统，研究了密度为1020株/hm^2的11年生火炬松秋季树干液流特征及其与环境因子的关系。结果表明：火炬松树干液流表现为单峰曲线，日出后，树干液流迅速上升，峰值出现在中午前后，日落后至次日日出前液流微弱或几近无上升液流；树干液流速度白天10时至17时平均约26cm/h，最大达到54cm/h；不同径阶火炬松树干液流流量差异极显著。4株样木的胸径分别为13．5，10．2，15．7，16．6cm，秋季耗水量分别为13．28，13．27，36．33，49．80kg/d。环境因子对树干液流的影响次序由大到小依次为：气温、相对湿度、光合有效辐射、土壤温度、土壤水分；逐步回归建立的方程达到极显著水平。     

中药有效化学成分促进干细胞增殖与分化作用研究进展

本文介绍传统中医药中有效化学成分调控干细胞的增殖、分化作用的近期研究进展。     

医用历四柱干支显现的基本规律

本文依据天文万年历研究了医用历四柱干支显现的基本规律：（１）共有５１８４００种四柱干支组;历经一个６０甲子年显现的一半;历经２个６０甲子年显现的３／４;历经３个６０了年大多数显现;历经４个６０甲子年才全部显现。（２）四柱干支历经４个６０甲子年基本重现,医用历轮回周期的２４０年。     

Cadherin-based biomaterials: Inducing stem cell fate towards tissue construction and therapeutics

The precise regulation of cell fate by biomaterials is one of the core focuses in tissue engineering and regenerative therapeutics. Cell fate is controlled by the niche signaling cues, including growth factors/morphogens, direct cell-cell contact, and extracellular matrix components. Here, we discuss how to bio-mimic cell-cell interaction by tailoring the interface of biomaterials to design effective scaffolds. Cadherins, as a key cell membrane protein family mediating the intercellular adherens junction, have attracted extensive attention in the functional modification of biomaterials. Therefore, cadherin-based biomaterials, their ability to modulate stem cell fate, and their applications in regenerative medicine are reviewed. Furthermore, we provide an outlook for exploiting the bio-mimic biomaterials in advanced cell therapeutics.     

视神经损伤治疗的进展

视神经损伤以视网膜神经节细胞(RGCs)的丢失为主的病理变化导致了视功能障碍,随着一定数量存活的RGCs在合适环境下的修复再生,视功能可有一定的恢复,综述相关研究文献,旨在了解视神经损伤及修复再生可能存在的规律,以及促进其再生的方法,客观评价视功能恢复的情况。     

Neuroreplacement therapy and stem cell biology under disease conditions

Recent advances in stem cell technology are expanding our ability to replace a variety of cells throughout the body. In the past, neurological diseases caused by the degeneration of neuronal cells were considered incurable because of a long-held 'truism'; neurons do not regenerate during adulthood. However, this statement has been challenged, and we have now found much evidence that the brain is indeed capable of regenerating neurons after maturing. Based on this new concept, researchers have shown neural differentiation of stem cells and recovery of function following transplantation of these cells into the brain. These results may promise a bright future for clinical applications of stem cell strategies in neurological diseases; however, we must consider the pathophysiological environments of individual diseases that may affect stem cell biology. Before we begin to develop clinical applications, we must consider environmental factors that have not been discussed in the current preclinical studies. Here, we study cases of Alzheimer's disease and schizophrenia and discuss the effects of environmental factors under disease conditions.Received 15 January 2003; received after revision 7 April 2003; accepted 8 April 2003     

栀子花试管快速繁殖的研究

栀子花具芽茎段在适宜浓度的ＢＡ，Ｋｔ与ＮＡＡ的ＭＳ培养基中，可获得增殖芽丛。切割芽丛继代培养进行苗的增殖。ＮＡＡ利于出根，ＩＢＡ有助于根的伸长。２０ｄ试管苗生根率达１００％，小苗移栽成活率达８５％－９０％。     

转基因动物及其应用

转基因动物技术是近十余年发展起来的,它为生命科学研究从微观的分子水平到宏观个体水平的联系提供了一套有用的工具和模型,在基本理论和实际应用研究上都很有价值.本文介绍了转基因动物的基本概念,基本制备方法以及此技术在诸多方面的应用.     

干细胞的生物学基本特征及其应用价值

本文试述干细胞的生物学基本特征及其应用价值,以便于进一步认识干细胞,更好地为教学、研究服务。