联苯衍生物紫外光谱的CNDO/S量子化学计算

利用CNDO/S程序对联苯及其衍生物的紫外光谱进行了量子化学研究,算出了各分子轨道的能量及相应紫外光谱吸收峰的理论位置,并与实验值进行了比较,指出了K吸收带为π-π~°跃迁,B吸收带为π-π~°或π-π~°′跃迁。     

Re-evaluation of the phylogenetic position of the genus Dexiotrichides (Protozoa, Ciliophora, Scuticociliatida) inferred from stomatogenetic and molecular information for Dexiotrichides pangi

The divisional process and systematic position of the marine scuticociliate Dexiotrichides pangi are studied. Based on both stomatogenetic data and 18S rDNA gene sequences, the phylogeny and morphogenetic characteristics of this taxon, and of other related genera, are analyzed and discussed. Both the divisionary events and the molecular biological data indicate that this speciesgenus, together with certain other genera in the Dexiotricha-complex, occupies an intermediate position between the tetrahymenids and the “typical” scuticociliate, which suggests that the Dexiotricha-like taxa should be excluded from the “true” scuticociliates. As a further contribution, the process of stomatogenesis in D. pangi can be summarized as follows: (1) The oral primordia in the opisthe are formed only by the proliferation of basal bodies in the scutica field, which subsequently develop into three membranelles, while the new paroral membrane seems to be generated by the sub-anterior portion of somatic kinety 1 (the 1st postoral intercalary kinety). The latter character exhibits a mode similar to Tetrahymena. (2) In the proter the parental membranelles are retained and remain unchanged throughout the entire division process; only the old paroral membrane is disassembled and differentiated into the anlage, which then gives rise to the new paroral membrane and the scutica of the proter. The 18S rRNA gene sequence reported here is the first one for a ciliate in the Dexiotricha-complex.     

Two virus-encoded RNA silencing suppressors, P14 of Beet necrotic yellow vein virus and S6 of Rice black streak dwarf virus

Functional analysis for gene silencing suppressor of P14 gene of Beet necrotic yellow vein virus and S6 gene of Rice black streak dwarf virus was carried out by agro- infiltration with recombinant vectors of Potato virus X. The phenotype observation of green fluorescent protein (GFP)expression and Northern blot showed that the gene silencing of gfp transgenic Nicotiana benthamiana induced by homologous sequence was strongly suppressed by the immixture infiltration of either the P14 or the $6. In the suppressed plants, the gfp mRNA accumulation was higher than that in the non-suppressed controls and the symptoms caused by PVX infection became more severe, especially the gfp DNA methylation of plant genome was significantly inhabited when co-infiltrated with RBSDV S6 gene. These results suggested that these two virus genes were potentially to encode for proteins as RNA silencing suppressors.     

西藏冬虫夏草无性型5.8S rDNA,ITS间区系统发育分析

 采用相对较低的培养温度(5~12℃),从西藏那曲当年产风干冬虫夏草[Cordyceps sinensis(Berk.)Sacc.]分离得到菌株C21,综合其菌丝形态学特征及5.8S rDNA和ITS间区的系统发育分析结果,证实菌株C21为冬虫夏草菌的无性型,即中国被毛孢(Hirsutella sinensis).     

基本蚁群算法的A.S.收敛性研究

蚁群算法是近几年优化领域中新出现的一种启发式仿生类并行智能进化算法，虽然该算法已经在众多组合优化领域中得到广泛应用，但是对其收敛性尤其是A．S．（AlmostSurely）收敛性问题的研究还存在很多空白．本文在介绍蚁群算法基本原理的基础上，以Markov链和离散鞅作为研究工具，对基本蚁群算法的A．S．收敛性问题进行了理论证明，把最优解集序列转变为下鞅序列来考察残留信息素轨迹向量的收敛性，随后提出了基本蚁群算法首达时间的定义，并对基本蚁群算法首次到达时间的期望值进行了理论分析．     

美国基督教长老会的传教活动对赛珍珠创作的影响

作为美国南长老会传教士一员，赛珍珠尽管对海外传教活动心存疑虑，但她的创作却与之有着千丝万缕的联系。传教活动让赛珍珠认识了中国，影响了她的多元文化观的形成，并使她一生都持有宗教宽容的立场。     

档案集成管理信息系统B/S运行模式研究

网络技术的普及，极大地带动了档案信息化的发展，各级档案管理部门都纷纷规划并实施其信息化战略，以适应新时期档案管理工作的需要。B／S运行模式的档案管理信息系统在应用中应注意重视档案信息基础工作、档案系统工程建设、人才队伍建设、传统载体档案管理工作。     

重组SARS冠状病毒S蛋白的原核表达研究

目的克隆表达SARS冠状病毒的主要结构蛋白(S蛋白)。方法合成SARS冠状病毒S蛋白特异性基因片断并克隆入pET32a原核表达载体,转化BL21菌,经IPTG诱导高效表达得到重组S蛋白,并通过W estern印迹对重组蛋白质进行鉴定。结果重组蛋白质经镍柱亲和层析得到了部分纯化,免疫动物后得到SARS病毒的多克隆抗体。结论经E lisa检测,表达的重组S蛋白基本具备检测病人血清中抗SARS病毒IgG和IgM的能力,可进一步用于S蛋白功能研究与SARS诊断试剂盒的研制。     

饲草压捆机压缩活塞结构ANSYS分析及压缩理论

在饲草压捆机设计过程中,对其压缩的关键工作部位--压缩活塞,采用了先进的工业设计软件Pro/Engineer和ANSYS有限元分析软件进行了结构分析,优化了设计.同时,通过研制样机现场工作试验,分析了饲草在压缩过程中的变化规律,丰富了饲草压缩理论,为改进、完善饲草压捆机的设计提供了理论依据.     

SIP UA在S3C2410X硬件平台上的设计与实现

在局域网内用SIPUA电话系统替代传统的PBX电话系统,不但有利于降低设备运行维护成本,而且有利于建立高效的综合服务内部网.针对SIPUA的功能要求,采用“硬件 嵌入式操作系统 应用层软件”的系统架构,设计了以三星S3C2410X微处理器为核心的硬件开发平台,并在此硬件平台上实现了SIPUA系统.