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To examine the community structure of β-Proteobacterial ammonia-oxidizing bacteria (AOB) in prawn farm sediment, the 16S rRNA gene library was constructed with β-Proteobacterial AOB-specific primers. The library was screened by PCR-restriction fragment length polymorphism (RFLP) analysis and clones with unique RFLP patterns were sequenced. Two groups of β-Proteobacterial AOB, the Nitrosomonas and the Nitrosospira, were detected. The Nitrosomonas occupied an absolute dominant position, accounting for more than 90% of total clones in the clone library, while the Nitrosospira accounting for 5.48%. Nitrosomonas-affiliated clones were grouped into the Nitrosomonas marina and the Nitrosomonas sp. Nm143 clusters, and Nitrosospira-affiliated clones were grouped into the Nitrosospira cluster 1. No other clusters of β-Proteobacterial AOB were found. The results enriched our knowledge of AOB diversity in the prawn farm sediment and provided important foundational data for further functional studies of these microbes in mariculture environments. 相似文献
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This study was conducted on the desert riparian forest along the lower reach of the inland Tarim River, which is located in the arid region of Northwest China. Fifteen plant species in 10 families were collected from five monitoring sections, and examined for the infection ofarbuscular mycorrhizal fungi (AMF). The impact of different soil factors on AMF infection rate and intensity was compared using the principal component analysis (PCA) method. The results indicate that 11 species are AM and only 4 are non-AM plants. The estimated capacity of AMF infection depends on families of plants and also the parameters (infection rate, infection intensity, fungal spore density) used. The density of fungal spores was relatively higher in Phragmites communis and Populus euphratica in Graminaceae and Salicaceae families, respectively. The infection rate was above 50% in all the AM plants, except Calligonumjunceum. The highest infection rate appeared in Alhagi sparsifolia (97%) and Glycyrrhizainflata (92%). However, when compared by AMF infection intensity, Tamarix spp. became the top one, followed by Alhagi sparsifolia, and Glycyrrhiza inflata was in the middle range of all the species. The PCA has identified that soil total salt, moisture, organic matter, total nitrogen, total P, available K and pH were closely associated with the AMF infection. 相似文献
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杨桂荣 《内蒙古民族大学学报(自然科学版)》2014,(4):461-462
目的:探讨儿童腹泻的病原检验结果,以此为基础进行儿童腹泻的病原分析并提出治疗意见.方法 :对内蒙古民族大学附属医院824例腹泻患儿的病原检验结果进行回顾性分析,总结常见病原体.结果:所有患儿中,病毒感染率为45.75%,主要感染病毒为轮状病毒和腺病毒;细菌感染率为12.38%,主要感染细菌为沙门菌和志贺杆菌.结论:导致儿童腹泻的主要病原为病毒,且以轮状病毒感染为主,次要病原为细菌感染,因此,临床治疗时需要根据检验所得出的患者的实际情况进行治疗. 相似文献
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<正>冬季气温变化大,人容易感冒。一般情况下没什么大碍,却让人非常难受。感冒药因此成了家庭药箱中举足轻重的角色,堪称药物界"最熟悉的面孔"。然而,使用感冒药的门道你都了解吗?感冒是种"不治之症"感冒是一种上呼吸道感染,是由病毒引起的,也就是说只能杀灭细菌的抗生素对感冒不会起作用。盲目使用抗生素可能增加不良反应和肠道细菌的耐药情况,有害无利。即使在患感冒的同时发生了细菌感染,需要使用抗生素,也必须由医生决定如何使用。 相似文献
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Angèle Nzoué 《自然科学进展(英文版)》2008,18(1):43-49
The aim of this study is to investigate the diversity of Retama raetam root-nodule bacteria isolated from arid regions of Tunisia. Twelve isolates, chosen as representative for different 16S rRNA gene patterns, were characterized by 16S rRNA gene sequencing and phenotypic analysis. Isolates were assigned to Sinorhizobium, Rhizobium and Agrobacterium. Symbiotic properties of Sinorhizobium and Rhizobium isolates showed a large diversity in their capacity to infect their host plant and fix atmospheric nitrogen. Strain RK 22 identified as Rhizobium was the most effective isolate. 相似文献
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不久前,美国加利福尼亚州生产的菠菜因为沾染了大肠杆菌而导致流行疾病爆发,随后,又有好几个州发生了沙门氏菌中毒事件。在这些事件发生之前,美国俄克拉荷马州立大学的科学家便开始研究利用高科技预防食品细菌感染的方法,现在,这项研究已取得了不小的进展。 相似文献
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目的 探讨血清降钙素原(PCT)在小儿肾病综合征复发中的意义.方法 将我院收治的原发性肾病综合征复发的患儿分为三组即合并细菌感染组50例、合并病毒感染组32例及非感染组16例,采用免疫色谱法,分别测定三组患儿的血清PCT,同时测定C反应蛋白(CRP)、中性粒细胞碱性磷酸酶(NAP)及外周血白细胞(WBC)计数.结果 细菌感染组血清PCT阳性率(84%)高于非感染感染组(12.5%)和病毒感染组(0%),差异均具有非常显著统计学意义(P<0.01).细菌感染组血清CRP阳性丰(76%)高于非感染感染组(37.5%)和病毒感染组(0%).差异均具有非常显著统计学意义(P<0.01).细菌感染组血清NAP阳性率.(78%)高于非感染感染组(31.3%)和病毒感染组(12.5%),差异均具有非常显著统计学意义(P<0.01).细菌感染组WBC计数均值高于非感染感染组和病毒感染组,差异均具有非常显著统计学意叉(P<0.01).在细菌感染组.血清PCT检测阳性率高于CRP、NAP检测阳性率,差异具有统计学意义(P<0.05).结论 血清PCT检测可应用于肾病综合征复发患儿,作为早期快速鉴别细菌感染的实验室新指标,以明确肾病复发的原因,指导抗生素的使用. 相似文献
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分析产后感染治疗,常见的产后感染为生殖器系统感染、尿路感染及乳腺感染。在进行抗菌素治疗以前必须先作详细的体格检查和化验,包括乳房检查、阴道高位涂片及培养b导尿液培养和尿液化验。致病菌一早分离,就立即作对抗菌素的放染试验,作为进一步选择药物的依据,对注射用头孢噻肟钠配舒巴坦钠预防和治疗妇产科感染的安全性、有效性及不良反应进行分析。 相似文献