我国科学家绘制完成水稻全基因组“精细图”

我国科学家日前绘制完成水稻全基因组“精细图”并进行了相关研究，有关成果发表在国际期刊(PLoS Biology(科学公共图书馆-生物学专辑）》2005年第3卷第2期上。专家认为，水稻基因组”精细图”的完成，为科学家研究各个水稻亚种的差异，在新层面上探讨杂交优势的机理，为禾本科植物的比较基因组学和进化研究奠定了基础。     

柔嫩艾美尔球虫厦门株(Eimeria tenella)生物学研究

采用单卵囊分离方法从厦门地区饲养的鸡盲肠中获得艾美尔球虫,经显微观察、测量和感染实验,从寄生部位、卵囊形态、孢子化时间和潜伏期等指标测定,实验结果:采用琼脂板方法分离和挑选单卵囊方法进行感染效果理想,单卵囊感染小鸡成功率为60%.该球虫寄生在鸡盲肠部位,其余部位未发现,卵囊形态长卵圆形,长25.80(23.5～28.5),宽20.86(18.5～23.5),长/宽:1.23(1.21～1.27),27℃条件下孢子化时间为20 h.,潜伏期为146 h.与国内一些株进行比较存在差别,确定该艾美尔球虫为柔嫩艾美尔球虫(Eimeriatenella),并定名为柔嫩艾美尔球虫厦门株.     

精巢间质细胞雄激素合成及其调节的研究进展

本文综述了近年来精巢间质细胞雄激素的合成及其分泌调节的研究进展。间质细胞内的雄激素合成的限速步骤是胆固醇转化为孕烯醇酮时的胆固醇跨线粒体膜转运。在此转运过程中，PBR可能是作为依赖于配体的通道蛋白，对胆固醇分子进行跨膜转移；StAR则通过解离线粒体膜与胆固醇分子的吸附作用，加速了胆固醇分子的转运。雄激素的合成还受垂体、支持细胞、巨噬细胞和其自身分泌激素和细胞因子的调控。同时对今后研究的方向做了总结和展望。     

齿瓣延胡索细胞悬浮系的建立和延胡索乙素的形成

就各种因素对齿瓣延胡索悬浮细胞生长和延胡索乙素形成的影响做进一步的研究。结果表明：随着接种量的提高，细胞生长加速，培养液浊度增大，齿瓣延胡索细胞悬浮液培养中最适接种量为2g/30ml;pH5.6最有利于细胞生物量的积累；2，4—D与2种细胞分裂素的组合，对于生长量积累和THP含量的作用最显著；在培养基中添加甘露糖和半乳糖，对于提高培养物THP含量是有效的；G1y Phe Lys的作用效果最显著，细胞鲜质量、干质量和THP含量均最高。     

铂(II)与胺类配合物的合成、晶体结构和抗肿瘤活性研究

研究铂(II)与三种胺类配合物的合成、晶体结构和体外抗肿瘤活性,化合物1的X-射线单晶结构分析表明:这是一个很少见的具有四方锥几何构型的铂(II)配合物,顺式平面四方形配位的铂的轴向上有第5个配位原子。其抗肿瘤活性实验显示3个配合物对几种研究的癌细胞都有较强的抑制作用。     

Some Properties of Fractals Generated by Linear Cellular Automata

Fractals and cellular automata are both significant areas of research in nonlinear analysis. This paper studies a class of fractals generated by cellular automata. The patterns produced by cellular automata give a special sequence of sets in Euclidean space. The corresponding limit set is shown to be a fractal and the dimension is independent of the choice of the finite initial seed. As opposed to previous works, the fractals here do not depend on the time parameter.     

生物毒素研究与应用展望

生物毒素是一类具有重要意义的生物源化学物质，已知化学结构的毒素可达数千种。生物毒素表现出生源、化学结构、作用机制等多方面的多样性。生物毒素研究已发展成为与多种学科交叉的新学科——毒素学，其发展对于生命化学、生源合成化学、化学生物学、化学生态学、医学、药物学、环境科学均有重要意义，对药物创新研究更具积极作用。许多生物毒素可作为临床药物或导向化合物，并可为药物分子设计提供有价值的新药效模型和结构构架，更能为发现药物新作用靶位发挥特殊作用。     

Effects of hyaluronic acid－chitosan－gelatin complex on the apoptosis and cell cycle of L929 cells

With the development in the field of tissue engineering, the interaction between biomaterials and cells has been deeply studied. Viewing the cells seeded on the surface of materials as an organic whole, cell cycle and apoptosis are analyzed to deepen the study of cell compatibility on biomaterials, while cell proliferation and differentiation are studied at the same time. In this paper, hyaluronic acid is incorporated into the chitosan-gelatin system. Propidium iodide (PI) was used in cell cycle analysis and the double-staining of cells with annexin-V and PI was applied in cell apoptosis analysis. The results show that incorporated hyaluronic acid shortens the adaptation period of cells on the material surface, and then cells enter the normal cell cycle quickly. In addition, added hyaluronic acid inhibits cell apoptosis triggered by the membranes. Therefore, hyaluronic acid improves the cell compatibility of chitosan-gelatin system and benefits the design of biomimetic materials.     

Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.