Proteomics study of the effects of high pigment-1 on plastid differentiation during the ripening of tomato fruits

The chloroplast-to-chromoplast difference is a highly regulated process. Tomato （Solanum lycopersicum） high-pigment mutant hp-1 shows an enhanced accumula- tion of carotenoids coupled with an increase in the volume and number of plastids. However, how chromoplasts of hp-] acquire their specific metabolic characteristics is still unclear. A comparison of proteome profiles from plastids at the mature green, breaker, and red stages of tomato fruits showed 45 differentially expressed proteins. These identi- fied proteins fell into six different functional categories. Our results showed that most of the proteins related to the Calvin cycle increased transitorily only at the early breaker stage, and remained unchanged at the early red stage. We found 18 proteins that were differentially expressed between the wild type and hp-1. The abundance of GCPE in chromoplasts of hp-1 was higher than that in the wild type. Meanwhile, we found that heat shock proteins were only present specifically in the mature green stage chromoplasts of hp-1 but not in the wild type. This suggested that GCPE and heat shock proteins might play important roles in the accumulation of high carotenoids in hp-1. Overall, our results could be helpful for understanding chloroplast-to-chromoplast differentiation and the function of hp-1.     

成人急性白血病淋巴细胞HSP70蛋白及mRNA的表达研究

研究热休克蛋白70(HSP70)在成人急性白血病淋巴细胞中的表达。用W estern blot法检测化疗前、后及正常人HSP70的蛋白表达,RT-PCR法检测其HSP70mRNA的表达。化疗前成人急性白血病淋巴细胞HSP70蛋白表达明显低于正常人(P<0.05);其HSP70mRNA表达的灰度值为(0.112±0.005),明显低于正常(人0.198±0.002)(P<0.05);而化疗后,成人急性白血病淋巴细胞HSP70及其mRNA(0.287±0.001)明显高于正常人(0.198±0.002)(P<0.05)。HSP70在成人急性淋巴细胞白血病淋巴细胞中高表达,对癌细胞起保护作用。     

果蝇对冷、热极限温度耐受能力的研究

以野生型黑腹果蝇(Drosophila melanogaster)为基本实验材料．统计分析了非致死的冷、热预刺激对果蝇耐极限温度能力的影响．并用SDS-PAGE电泳研究了冷、热刺激后热休克蛋白的表达情况．实验发现．冷、热预刺激能提高果蝇在同类温度下的耐受水平．同时，高、低温具有交互作用．即冷预刺激提高了果蝇在高温下的存活率．在0℃下．果蝇体内22、23、26、27、42、51、52及70kD的蛋白质出现增量表达；37℃下70、83kD的蛋白质表达量增多．推测上述增效作用可能是这些蛋白质引起的．     

创伤性休克的护理体会

报告了46例创伤性休克患者的护理过程。     

热休克后小鼠血红蛋白和血清蛋白的初步研究

本文采用人工气候室模拟自然热环境对小白鼠进行急性热暴露实验．测定热休克后不同时程小白鼠血中血红蛋白含量和血清中总蛋白含量变化，同时对血清蛋白进行电泳分析．结果表明，热休克后不同时程血红蛋白含量和血清总蛋白含量出现规律性改变，血清蛋白电泳图谱未见明显改变．作者对实验结果的意义做了分析．     

热休克蛋白及其在生殖中的作用

本文简述了热休克蛋白的发现分类和调节以及其在生殖中的作用。     

阿片δ_1受体激动剂对出血性休克大鼠心肌损伤的影响

为观察出血性休克后复苏开始前给予阿片δ1受体激动剂2-Methyl-4aa-(3-hydroxyphenyl)-1,2,3,4,4a,5,12,12aα-octahydroquinolino[2,3,3-g]isoquinoline dihydrobromide(TAN-67)对大鼠心肌缺血缺氧性损伤的影响.将24只成年雄性SD大鼠,随机分为三组,每组8只,实验分假手术组、休克对照组、TAN-67组.休克对照组和TAN-67组大鼠放血使MAP降至40 mmHg,休克持续60 min,注射生理盐水或TAN-67后,回输自体血进行复苏,观察复苏2 h内MAP和心功能(LVP、±dp/dtmax)的变化.实验结束时取心肌组织行病理学电镜检查.并分析各组线粒体损伤.休克对照组复苏后各时点血流动力学指标呈逐渐下降趋势.TAN-67组复苏后同一时点各项指标均显著高于对照组(P<0.05).病理学电镜检查和线粒体损伤结果,TAN-67组心肌损伤较对照组减轻.结果表明TAN-67有利于急性出血性休克大鼠血流动力学复苏,对心肌损伤有保护作用.     

肉苁蓉总苷对兔失血性休克／再灌注的影响

研究肉苁蓉总苷（GC）对兔失血性休克/再灌注（S/R）损伤的保护作用及其机制，用家兔S/R模型，观察血浆中丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性和血栓素A2（TXA2）、前列环素（PGI2）代谢产物的浓度以及GC对上述指标的影响，结果表明：与S/R组比较，GC治疗组可使血浆中MDA和TXB2（TXA2的代谢产物）明显降低，SOD和6-酮-PGF1α（PGI2的代谢产物）明显升高，说明GC对S/R损伤有保护作用。     

成年休克病人静脉置管巧置法

浅静脉穿刺是临床护理最基本的操作技能之一，尤其是休克病人在外科急危重症抢救时候要达到一针见血也是值得不断总结、探索的技术，现结合工作实践就如何提高静脉穿刺成功率进行探讨。