Tobacco curly shoot virus isolated in Yunnan is a distinct species of Begomovirus

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Yl is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).     

毛细管反相电色谱中电渗流测定方法的研究

根据反相高效液相色谱中同系物保留值的碳数规律,通过迭代分析的方法测定了毛细管电色谱中的电渗流速率,并与一般的测定方法加以比较,探讨了影响电渗流速率测定结果的可能原因。     

用光探针法测量表面复合速率

通过系统的研究分析,建立了应用光探针的光电（ＰＣ）测量方法,应用镜像法和点源产生近似原理建立了物理模型,并进行了系统的理论分析．导出了光电流与表面复合速率相应关系的计算公式,确定了可进行电量测量的实验装置．采用此方法,测量了台面型高压硅半导体器件的无机钝化和有机保护界面的表面复合速率．通过测量结果和计算结果的归一化比较,获得了其表面复合速率．     

山新杨蔗糖合酶基因PCR介导的重组现象研究

[目的]山新杨(Poputlus davidiana ×P.bolleana)是林木基因工程育种基础和应用研究的良好材料;以山新杨蔗糖合酶基因(PdbSUS)为例研究聚合酶链式反应(polymerase chain reaction,PCR)过程介导的重组现象,在此基础上区分每一个PdbSUS基因的两种单倍型,为后续研...     

一种基于蜂王交配的求解复杂问题的演化算法

在对传统演化算法分析的基础上,提出了一种基于蜂王交配的求解复杂优化问题的演化算法(QMSGA).它的特点在于:模拟自然界中蜂王的交配方法,并引入均匀杂交和平均矢量偏差变异因子策略来达到种群分布的多样性,最终求出复杂优化问题的全局最优解.实验结果表明,对于求解复杂的多峰优化、陡峭函数优化等问题,该算法比传统的演化算法具有更好的精确度和收敛速度.     

A Study on Recombination Efficiency of Commercial Bank Branch Based on DEA Approach

For commercial bank branch, improving efficiency is very important for enhancement of overall efficiency and appraising efficiency is also an urgent problem to resolve. The paper compared the efficiency of recombining branch with the efficiency of no recombining branch of 23 commercial banks by using the approach of output-max CCR model of Data Envelopment Analysis （DEA）. According to the empirical results of the study, we can make some conclusions. Firstly, bank branch recombination is based on the comprehensive raising of human sources, pecuniary resources and material resources, and can be inefficient under the immature condition. Secondly, the efficiency of commercial banks can be improved enormously because their inner management and resource allocation in recombining are wasted largely. Thirdly, nowadays, commercial banks should emphasize on recomposing resources, mending technical ＆ managerial innovation and improving management level generally.     

高离化类锂离子（Z=72～75）的态—态双电子复合

基于相对论多组态ＨＦ程序，采用畸变波理论和独立共振近似，计算了０．１～９．０ｋｅＶ温度范围的类锂高电荷离子Ｈｆ＾６９＋，Ｔａ＾７０＋，Ｗ＾７１＋和Ｒｅ＾７２＋对应于原子实△ｎ＝０，１激发的态－态双电子复合速率系数，讨论了复合速率系数随电子温度，原子序数，复合类型以及双激光发态中俘获电子的主量子数的变化关系，计算结果表明，不同复合通道的速率系数存在的很大差异，由此提出了通过双电子复合过程来实现高电荷     

多基因双T-DNA植物表达载体的构建及拟南芥转化

多基因植物表达载体用于植物遗传转化是培育具有多种优良品质作物的有效策略. 双T-DNA系统是实现筛选完成后选择标记基因删除的一种简便可行的方式. 为培育高度抗逆或去除标记基因的农作物，构建了多基因双T-DNA植物表达载体2T-bbgdD，其中含有一个抗除草剂基因bar, 3个抗逆相关基因(DREB1A, Na+依赖性Pi转运体基因（d5）, betA)和一个报告基因gfp. 利用农杆菌介导法将该载体转入拟南芥，获得了多基因共转化及去除标记基因的转基因拟南芥. 可将此植物表达载体进一步用于作物的遗传转化.     

Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast

The use of plants as bioreactors for the expression oftherapeutic proteins has developed into a new field in biotechnology research[1]. Plant systems provide simple genetic manipulation, low production costs, and low risk of contamination by animal viruse…     

热处理温度对AlP/SiO_2纳米复合材料发光的影响

对不同温度热处理的xAlP/1 0 0SiO2 纳米复合材料的光致发光进行了研究 .结果表明 ,50 0℃热处理的样品光致发光谱只有位于 585nm附近的发光峰 ,它来源于表面态和缺陷复合发光 .6 0 0℃热处理的样品有两个发光峰 ,分别位于 585nm和6 35nm附近 .分析认为 ,6 35nm处的发光是被弛豫至表面态的电子和空穴通过隧穿复合发光 ;材料热处理温度的提高有利于晶粒的生长 ,从而使表面态和缺陷发光明显减弱 ,出现另一由于隧穿复合产生的发光峰