高质量蛋白质空间结构数据库的构建方法

根据不同的研究需要,提出了不同的建库标准。在以分辨率为主要选择依据的基础上,采取综合的打分的方法,全面地衡量一个序列质量的高低。引入同一性因子Ｓ,找到了序列的同一性Ｓ和序列同源性Ｖ的关系,从而在判别序列之间同源程度时由序列的同一性因子Ｓ取代序列同源性参数Ｖ。     

Construction of the glucose isomerase deficient strain ofStreptomyces M1033 by homologous recombination

After the establishment of the transformation conditions ofStreptomyces diastaticus No.7 Strain M1033, the integration plasmid pXW for homologous recombination, which contains a 600 bp fragment of incompleteGI (G138P. G247D) gene, has been constructed in order to realize the stable overexpression of theGI (G138P. G247D) which is valuable for large-scale industrial production. The Gigene’s disruption has been realized by pXW’s integration into M1033 chromosomes via homologous recombination andGI deficient strain ofStreptomyces M1033 has been obtained. The reliability of introduction of mutation has been proved by analysis of recombinant fragment and affirmance of existence of the mutation, as well as detection of the stability of the deficient strain.     

Co-transformation to tobacco of Cre/lox site-specific recombination system and its precise recombination

For the temporally and spatially regulated expression of the barnase gene in plant,two kinds of plasmids with cre gene and its directly repeat recognition sites lox from bacteriophage P1 were constructed and co-transformed into tobacco by agrobacterium mediated procedure.The transgenic plants were conformed by PCR analysis.The blocking fragment between the two lox directly repeat sites was excised by Cre protein in the transgenic plant genome.Cloning and sequencing the DNA fragment from the co-transformed plant DNA showed that the precise DNA excision occurred in transgenic tobacco genome directed by Cre/lox site-specific recombination.     

重组降血压肽多肽序列的设计及表达系统构建

通过分析降血压肽结构与功能的关系,设计并合成5条降血压肽序列,通过活性测定以及体外消化试验,确定P3（六肽）为理想降血压肽,为实现该小肽在大肠杆菌中的表达,经特定酶切位点将其串联为6拷贝的融合多肽,将相应的基因序列克隆至表达载体pGEX-4T-2并转化至宿主茵Escherichia coli BL21中,双酶切、PCR以及测序结果均表明成功构建了重组质粒pGEX-4T-2-ACEIP。     

pn结太阳电池饱和电流密度的理论研究

为提高太阳电池的开路电压Vac和效率η,必须减小饱和电流Jo,据此,对影响Jo的各种因素进行了研究,推导了更为普遍的有限尺寸条件下太阳电池的表面复合速度和少子加速场对Jo影响的表达式,并且对两者的影响进行了数值模拟,直观地得出了以下结论：在保证消除重掺杂效应带来不良影响的条件下,增大发射区少子漂移场强,可减小,Jo,提高电池Vco;减少电池的表面复合速度,能提高电池的性能。     

预测模型组合开关

不同预测模型在同一时刻都存在不同的预测误差,最优组合预测的预测精度要比其他模型高,但并不是所有时刻误差都最小,有的时刻可能比单项预测误差还大,因此,在预测时就存在较大的风险.再组合预测可以降低预测风险,但方法比较复杂难以推广.针对这些问题,我们设计一种预测模型组合开关,根据数据的变化,不同时刻采用不同预测模型,使预测误差每一期都最小,或不是最大.实证结果表明,预测模型开关能够根据数据特征有效控制模型输出.     

Homologous recombination in plants

In plants three different approaches have been used to study homologous DNA recombination; extrachromosomal recombination (ECR) between transfected DNA molecules, intrachromosomal recombination (ICR) between repeated genes integrated into and resident at the genome and recombination between introduced DNA and homologous sequences in the genome (gene targeting). ECR is efficient (10^–1 to 10^–3) and occurs mainly during a limited time period early after transfection. It proceeds predominantly via nonconservative single-strand annealing. ICR, which in most cases is described best by the double-strand break repair model of recombination, occurs at frequencies of one event in 10⁵ to 10⁷ cells. ICR takes place throughout the whole life-cycle of a plant, in all organs and at different developmental stages. As there exists no predetermined germline in plants, somatic recombination events can be transferred to the next generation. Recombination frequencies are enhanced by DNA damage. Gene targeting, like ICR, occurs at low rates in plant cells. Almost nothing is known about the enzymes involved in homologous recombination in plants.     

Gene conversion in the chicken immunoglobulin locus: A paradigm of homologous recombination in higher eukaryotes

Gene conversion was first defined in yeast as a type of homologous recombination in which the donor sequence does not change. In chicken B cells, gene conversion builds the antigen receptor repertoire by introducing sequence diversity into the immunoglobulin genes. Immunoglobulin gene conversion continues at high frequency in an avian leukosis virus induced chicken B cell line. This cell line can be modified by homologous integration of transfected DNA constructs offering a model system for studying gene conversion in higher eukaryotes. In search for genes which might participate in chicken immunoglobulin gene conversion, we have identified chicken counterparts of the yeastRAD51, RAD52, andRAD54 genes. Disruption and overexpression of these genes in the chicken B cell line may clarify their role in gene conversion and gene targeting.     

晶闸管中少子寿命控制技术的分析研究

本文通过对掺 Au、掺 Pt、电子辐照、γ射线辐照实验样片的 DLTS 测量、分析,确定它们在 N 型硅片中产生的有效复合中心能级分别为 E_(c-0.54ev)E_(v+0.42ev)、E_(c-0.42ev)、E_(v+0.45ev)。根据 Shockley 单能级复合理论的计算及采用四种寿命控制方法制造的快速晶闸管电参数的测量、分析和比较,提出对这四种少子寿命控制技术的适当评价。     

Effect of intercalating mutagens on crossing-over inDrosophila melanogaster females

Summary In order to evaluate the effect of several intercalating compounds on crossing-over inDrosophila melanogaster females, acridine orange, acriflavine, chloroquine, ethidium bromide and quinacrine were fed separately to larvae ofy ct f/+++ genotype. Our results show that acridine orange, acriflavine and ethidium bromide increase significantly the recombination frequency at thect-f region and support the view that, for intercalating agents, there is a relationship between clastogenic activity and female recombination induction.