运动发酵单胞菌RecET重组系统的构建及应用

为了提高外源基因整合到染色体的效率,在已构建的运动发酵单胞菌-大肠杆菌穿梭载体基础上,构建了RecET表达质粒pSUZM3a-RecET.选取乙醇脱氢酶Ⅰ(adhA)基因作为靶基因,四环素抗性基因(Tcr)作为筛选标记基因,检测RecET重组系统在Z.mobilis中进行基因重组的可行性.将两端带有60bp adhA基因同源臂的四环素抗性基因片段电击转化含有RecET重组系统表达质粒的运动发酵单胞菌ZM4,获得adhA基因缺失突变菌株.对突变菌株adhA基因的PCR产物进行测序发现,adhA基因已被置换为四环素抗性基因.上述结果表明:RecET重组系统在运动发酵单胞菌中具有高效、便捷和可操作性,只需60bp同源臂即可完成同源重组.     

Positive correlation between recombination rate and nucleotide diversity is shown under domestication selection in the chicken genome

Positive correlation between recombination rate and nucleoUde diversity has been observed in a wide variety of eukaryotes on megabase scale. On the basis of genome-wide chicken genetic variation map generated by comparing three domestic breeds with wild ancestor and the positions of markers on the genetic linkage map, we found that SNPs rates were similar for all chromosomes while the recombination rates increased in micro chromosomes. In other words no correlation exists in chromosome size. Nevertheless, when we scanned the genome by calculating the values of each characteristic within non-overlapping windows, instead of single value for each chromosomes, the nucleoUde diversity was found to be significantly correlated with the recombination rate （r=0.27, P〈0.0005）. Furthermore, the significant association not only existed between these two features, but also existed between all 6 pairwise combinations of nucleoUde diversity, recombination rate, GC content and average gene length. This co-variation is very meaningful for the studies of sequence evolution.     

产辅酶Q10大肠杆菌工程菌的构建

克隆放射型根瘤菌ddsA基因,用于构建red重组的打靶片段,通过同源重组替换大肠杆菌基因组上的ispB基因,使合成辅酶Q8的大肠杆菌具备合成辅酶Q10的能力.通过强化辅酶Q合成过程中的ddsA、ubiA、ispA、idi等关键酶基因,可使大肠杆菌辅酶Q10的合成能力提高126.9%.综合分析表明,ddsA与ubiA为辅酶Q10合成的关键基因,ispA与idi为辅酶Q10合成的次关键基因.     

GDa-Si:H薄膜的—新发光峰

GDα-Si:H薄膜作为廉价大面积太阳电池的基本材料日益显示出广阔的前景,人们已进行了大量的研究工作。然而对α-Si:H基本性质的许多问题至今尚未解决。由于光致发光谱可以探测α-Si:H的带尾和其他局域态的性质,故对GDα-Si:H薄膜光致发光的研究是当前基础研究的一个重要课题。迄今为止,人们已经发现α-Si:H薄膜有五个发光峰:(1)主发光峰。尽管对     

N型GaAs单晶表面复合速度的宽谱光伏测算

提出适用于可见光全波段的光伏测算ＧａＡｓ单晶表面复合速度的新方法．实验结果表明，对于掺Ｓｉ的Ｎ型弱简并ＧａＡｓ单晶（ｎ０＝２．０×１０１７ｃｍ－３），其表面复合速度Ｓｐ＝１．６×１０５ｃｍ·ｓ－１，与有关报道基本一致．     

Tobacco curly shoot virus iso-lated in Yunnan is a distinct species of Begomovirus

Virus isolate Y1 was obtained from tobacco showing curly shoot symptoms in Baoshan, Yunnan Province. Whitefly transmission test and virion morphology observation showed that it is a begomovirus. In reactions with 14 monoclonal antibodies raised against begomoviruses, Y1 was readily differentiated from begomoviruses reported in China, Pakistan and India. The complete nucleotide sequence of DNA-A was determined, it contains 2746 nucleotides, with two ORFs in virion-sense DNA and four ORFs in complementary-sense DNA. Comparisons with total DNA-A, intergenic region and deduced amino acid sequences of individual ORFs showed that Y1 is a distinct Begomovirus species, for which the name Tobacco curly shoot virus (TCSV) is proposed. The total DNA-A of TCSV is most closely related to that of Tomato leaf curl virus from India (85% sequence identity). In contrast, the deduced coat protein of TCSV is most like that of Cotton leaf curl virus 72b isolate from Pakistan (98% amino acid sequence identity).     

基因转化创造植物杂种优势利用新方式的途径分析

普通核雄性不育性遗传方式能够满足对植物最佳雄性不育系选育的要求,如果能解决其不育系繁殖问题,将优于现有的其他杂种优势利用方式。借助基因工程技术,通过质体转化、位点特异性重组技术和特殊启动子的利用,操控可育基因和不育基因的表达,可能繁殖出100%不育株率的普通核雄性不育系,创造出普通核雄性不育性利用新途径。     

浅谈ERP在我国企业中的运用

介绍了ERP的概念和企业实施ERP的必要性及作用，分析了一些企业实施ERP项目失败的原因，指出了实施ERP应注意的问题。     

基于并行组合模拟退火的全局优化算法

针对目前优化方法求解非线性多极值优化问题时存在的不足，提出了并行组合模拟退火算法。在分析算法性能的同时用并行组合模拟退火算法对两个算例进行了求解，并与模拟退火算法、遗传算法进行了比较。结果表明，该方法简单、可靠，具有较高的精度和适应性。