动物麻醉法浅述

动物实验中常会遇到麻醉方法等问题，麻醉效果常受实验动物、麻醉剂品种、麻醉剂量以及操作者的技能程度等因素影响，麻醉不当不但会影响实验的操作，严重的还会引起实验动物的死亡，影响科研工作的进程。     

中国北部地区药用甘草的生态学特性及群落分类

本文报导我国北部药用甘草的生物学特性和群落学分类系统.目的是为人工驯化栽培提供生态学依据,并为甘草野生资源的利用和保护提供资料.     

Aldose reductase structures: implications for mechanism and inhibition

During chronic hyperglycaemia, elevated vascular glucose level causes increased flux through the polyol pathway, which induces functional and morphological changes associated with secondary diabetic complications. Inhibitors of aldose reductase (ARIs) have been widely investigated as potential therapeutic agents, but to date only epalrestat is successfully marketed for treatment of diabetic neuropathy, in Japan. Promising compounds during in vitro studies or in trials with animal models have failed to proceed beyond clinical trials and to everyday use, due to a lack of efficacy or adverse side effects attributed to lack of inhibitor specificity and likely inhibition of the related aldehyde reductase (ALR1). Knowledge of the catalytic mechanism and structures of the current inhibitors complexed with ALR2 are means by which more specific and tightly bound inhibitors can be discovered. This review will provide an overview of the proposed catalytic mechanism and the current state of structure-based drug design.     

Morphine but not fentanyl and methadone affects mitochondrial membrane potential by inducing nitric oxide release in glioma cells

We have observed that treatment of human glioma cells with morphine in the nanomolar range of concentration affects the mitochondrial membrane potential. The effect is specific to morphine and is mediated by naloxone-sensitive receptors, and is thus better observed on glioma cells treated with desipramine; moreover, the mitochondrial impairment is not inducible by fentanyl or methadone treatment and is prevented by the nitric oxide (NO) synthase inhibitor L-NAME. We conclude that in cultured glioma cells, the morphine-induced NO release decreases the mitochondrial membrane potential, as one might expect based on the rapid inhibition of the respiratory chain by NO. The identification of new intra-cellular pathways involved in the mechanism of action of morphine opens additional hypotheses, providing a novel rationale relevant to the therapy and toxicology of opioids.Received 19 August 2004; received after revision 16 September 2004; accepted 7 October 2004     

利多卡因和丁卡因混合液硬膜外麻醉用于剖宫产术

130例剖宫产术均采用1.6％利多卡因与0.14％丁卡因(内含1:20万u肾上腺素)混合液作连续硬膜外麻醉。利多卡因用量180～240mg,平均3.05～3.34mg/kg,丁卡因用量15.8～20.9mg,平均0.21～0.28mg/kg,本组麻药首剂量为10～12ml,15～20分钟即可达到麻醉的目的。均不用术前药。在胎儿取出前,对产妇一律未用强化麻醉药及全麻药,输液以平衡液为主,限制葡萄糖液输注,以免新生儿产生高血糖症。避免新生儿出生前受到药物的影响。     

计算药物-受体相互作用结合能的一种新方法

本文通过药物功能团在药物-受体相互作用中能量贡献的分析,得到了一个计算药物-受体相互作用结合能的计算式,用以简便地计算结合能,据此可推测药物与受体的相互作用情况。     

庆大霉素过敏反应三例报告

本文报告了卫生所近一年半临床中发生的庆大霉素过敏反应三例，通过讨论与阐述，提出了在使用该药物时应引起充分注意的几个问题     

表面吸附固定化酶及其应用

研究用无机物载体(活性炭、分子筛、硅胶)表面吸附固定植物酶(菠萝酶,木瓜酶)技术,以及固定化酶的活性寿命及其催化效能。将实验制得的固定化酶多次反复应用于催化胶液水解反应中。反应结果证实表面吸附固定酶在技术上是可行的,经济上是合理的。另外还采用“补酶”的方法,使吸附酶趋向平衡,以减少该固定化酶中酶的脱落。初步探讨了用与水不溶支撑体的共价结合法固定酶技术。