Introduction of pokeweed antiviral protein cDNA intoBrassica napus and acquisition of transgenic plants resistant to viruses

Using cotyledonary petioles as explants, the PAP cDNA controlled by wound-inducible promoter has been introduced intoBrassica napus by coculture withAgrobacterium tumefaciens and laser microbeam puncture respectively. Regenerated plants resistant to gentamycin have been selected out. PCR amplification and Southern blotting analysis indicated that PAP cDNA together with wound-inducible promoter had been integrated intoBrassica genome with transformation frequencies of 2.0% and 1.7% for two transformation methods respectively. The test of virus challenge showed that these transgenicBrassica plants were resistant in different degrees to mechanically inoculated TuMV.     

水稻总RNA反转录出Cy3-dUTP标记cDNA的研究

以提取的水稻叶总RNA为模板，Cy3-dUTP为标记，进行反转录，得出Cy3-dUTP标记的cDNA，对影响本实验结果的因素进行了探究。     

杉木茎段形成层组织cDNA文库的构建

为了分离和克隆与杉木木材形成相关的重要基因,笔者以杉木2~3年生枝条,5~6月份形成层活动期的茎段形成层组织为材料,用表达型噬菌体载体ZAP构建了cDNA文库。文库宿主菌为E.coliXL1-Blue,亚克隆空菌为E.coliXLOLR。试验结果表明:初级文库的库容量达到6.1×105pfu,扩增文库的滴度达到5.2×109pfu/mL,重组率达到95%,插入片段大小在0.5~3.0 kb。     

人类基因组计划的实施进展

简述了人类基因组计划实施的的主要技术路线及该项研究的进展．     

基于微分的cDNA基因芯片图像自动划格算法

针对cDNA基因芯片数据分析中划格对提取杂交荧光样点杂交强度信息的重要作用，提出了一种基于微分的cDNA基因芯片图像自动划格算法，采用NCBI上GEO数据库中cDNA基因芯片图像进行划格实验，验证了该算法的有效性。     

胚胎性横纹肌肉瘤中下调表达基因HumCyr61的分？…

分离和克隆胚胎性横纹肌肉瘤中下调表达基因ＨｕｍＣｙｒ６１。方法：利用计算机辅助的同源扩增策略，成功克隆一个长１８８７ｂｐ ｃＤＮＡ，并与ＧｅｎＢａｎｋ核酸数据库比较。结果：该基因与鼠生长因子诱导早期表达的Ｃｙｒ６１基因同源度为８２％，在蛋白质水平上的同源度高达９２％，其中对高级结构有重大贡献的半胱氨酸和脯氨酸等氨基酸残基高度保守。查新结果还显示，它包括在胚胎性横纹肌肉瘤细胞系ＲＤ中表达呈下调表达的     

Isolation of a vernalization-related cDNA clone (VRC) using mRNA differential display in winter wheat

Vernalization is an essential factor which affects the flowering development in cold-requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization, we examined mRNA populations in differently-treated plumules of winter wheat (Triticum aestivum L. cv Yanda 1817) using mRNA differential display. One vernalizationrelated cDNA clone (VRC), VRC54, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that VRC54 was a novel vernalization-related clone found in higher plant which not only might play an important role in the floral induction in vernalization-requiring plants but also was different from the cold-acclimatized genes.     

牦牛肌红蛋白的cDNA序列测定及其氧化特性的研究

通过对牦牛肌红蛋白的含量和cDNA序列的测定及心肌肌红蛋白氧化和脂类氧化相关性的研究，初步探讨了牦牛适应高原低氧环境的机理. 牦牛与黄牛的肌红蛋白cDNA序列比较，二者只有2个碱基的差异（89位氨基酸由cac变为cat，91位氨基酸由gcc变为gct），但由于氨基酸密码子简并性，两者的氨基酸序列没有差异.牦牛的骨骼肌的肌红蛋白含量为488.3 nmol/g，与黄牛相比没有显著差异，但牦牛心肌的肌红蛋白含量为823.4 nmol/g，比黄牛高61.2％（P＜0.05）.在4 ℃贮存下，牦牛心肌中肌红蛋白的氧     

人粒细胞集落刺激因子cDNA的克隆与表达

用脂多糖诱导正常人单核细胞使产生粒细胞集落刺激因子。从诱导细胞中提取出总ＲＮＡ，以特上物经逆转录ＰＣＲ扩增出粒细胞集落刺激因子ｍＲＮＡ和ｃＤＮＡ，将其插入经改造的分泌型表达载体ｐＩＮ－ｏｍｐ Ａ，并转化受体菌Ｅ．ｃｏｌｉ。克隆的ｃＤＮＡ通过限制性酶双酶切的３＇端、５＇端及中间序列探针分子杂交鉴定，结果与预期一致。表达质粒在大肠杆菌哟ＩＰＲＴＧ诱导，粒细胞集落刺激因子被分泌到细菌的周质中。产物提取后