局部G-凸一致空间内的聚合不动点和具有U-优化对应的广义对策平衡

应用作者得到的一个聚合不动点定理，在局部G－凸一致空间内对具有无限多个经济人，具有非紧策略空间和具有 －优化对应的定性对策和广义对策，证明了某些平衡存在性定理。这些定理改进和推广了文献中某些已知结果。     

CDMA软切换的队列模式

针对移动通信系统的软切换技术，讨论了几种情况相应的算法思想，重点分析了基于方向的软切换模式，为更好地适应第三代、第四代移动通信系统的要求，提出基于方向的双队列模型，并定性地分析了该模型的性能，改善了系统的通信质量。     

HAb18G/CD147-mediated calcium mobilization and hepatoma metastasis require both C-terminal and N-terminal domains

HAb18G/CD147 is a heavily glycosylated protein containing two immunoglobulin superfamily domains. Our previous studies have indicated that overexpression of HAb18G/CD147 enhances metastatic potentials in human hepatoma cells by disrupting the regulation of store-operated Ca²⁺ entry by nitric oxide (NO)/cGMP. In the present study, we investigated the structure-function of HAb18G/CD147 by transfecting truncated HAb18G/CD147 fragments into human 7721 hepatoma cells. The inhibitory effect of HAb18G/CD147 on 8-bromo-cGMP-regulated thapsigargin-induced Ca²⁺ entry was reversed by the expression of either C or N terminus truncated HAb18G/CD147 in T7721C and T7721N cells, respectively. The potential effect of HAb18G/CD147 on metastatic potentials, both adhesion and invasion capacities, of hepatoma cells was abolished in T7721C cells, but not affected in T7721N cells. Release and activation of matrix metalloproteinases (MMPs), MMP-2 and MMP-9, were found to be enhanced by the expression of HAb18G/CD147, and this effect was abolished by both truncations. Thapsigargin significantly enhanced release and activation of MMPs (MMP-2 and MMP-9) in non-transfected 7721 cells, and this effect was negatively regulated by SNAP. However, no effects of thapsigargin or SNAP were observed in T7721 cells, and expression of HAb18G/CD147 enhanced secretion and activation of MMPs at a stable and high level. Taken together, these results suggest that both ectodomain and intracellular domains of HAb18G/CD147 are required to mediate the effect of HAb18G/CD147 on the secretion and activation of MMPs and metastasis-related processes in human hepatoma cells by disrupting the regulation of NO/cGMP-sensitive intracellular Ca²⁺ mobilization although each domain may play different roles.Received 1 April 2004; received after revision 15 June 2004; accepted 22 June 2004     

无类囊体蓝藻Gloeobacter violaceus基于广宿主质粒的遗传转移系统

无类囊体蓝藻Gloeobacter violaceus的细胞有厚胶质鞘包裹并形成群体,难以进行遗传操作.用注射器反复吸打的方法将胶质鞘剥落,得到大量单个细胞,通过接合转移将外源广宿主质粒pKT210转入G.violaceus.用斑点杂交方法以及对质粒进行酶切图谱分析,均证实外源质粒pKT210确已导入G.violaceus并稳定复制,未发生结构上的变化.从G.violaceus提取的pKT210可转化mcr和mrr基因缺陷的大肠杆菌DH10B却不能转化mcr+mrr+菌株DH5a,表明G.vio-laceus中可能存在甲基化酶系统.     

Nitric oxide mediates histamine induced down-regulation of H<Subscript>2</Subscript> receptor mRNA and internalization of the receptor protein (R1)

During agonist-dependent long-term stimulation of cells, histamine receptor subtypes are frequently down-regulated. However, the mechanisms underlying the modulation of receptor expression during long-term histamine stimulation have yet to be resolved. Based on our recently reported results showing an H₁-mediated down-regulation of histamine H₂ receptor mRNA in endothelial cells, our aim was to characterize the mechanism controlling rapid and long-term histamine-mediated modulation of H₂ receptor expression in more detail. We were able to show that the histamine-induced down-regulation of H₂ receptor mRNA and cell surface expression lasting for 24 h was accompanied by augmentation of the receptor protein level in the cytoplasmatic fraction of endothelial cells for this time period. Furthermore, changes in receptor protein levels in whole-cell lysate were negligible, indicating that the rapid and prolonged modulation of cell surface H₂ receptor levels by histamine was regulated solely via internalization. The role of nitric oxide (NO) as a key mediator in histamine-stimulated cell responses was underlined by subsequent studies showing the attenuation of histamine-induced H₂ receptor mRNA down-regulation and protein trafficking following NO synthase isozyme inhibition.Received 11 March 2003; received after revision 11 June 2003; accepted 17 June 2003     

G蛋白偶联受体研究进展

G蛋白偶联受体(Gprotein-coupled receptors,GPCRs)是具有7个跨膜螺旋的蛋白质受体,是人体内最大的蛋白质家族。按GPCRs一级结构的同源性,主要分为A、B、C三族;按氨基酸序列的相似性以及与配基的结合情况GPCRs可以分为5个亚家族。本文就GPCRs的结构、分类、GPCRs的二聚化以及其固有活性等方面做了一些介绍。     

210国道对牛背梁保护区羚牛东西扩散影响的研究(Ⅱ)——210国道车流量变化规律

2000年10月—2001年10月,采用定点观测的方法,对210国道保护区段每日04:00—22:00时的流动车辆进行了观测,总计流动车辆65 755辆,按观测日数计算,日均车流量782.8辆,平均1.37辆/2 min.每日15:00时的车流量最大,06:00时的车流量最少;主要集中在09:00—22: 00时,而以14:00—18:00时最为集中;每日的车流量变化可以分为四个阶段:即04:00—07:00时、07:00—13:00时、13:00—19:00时和19:00—22:00时,其中13:00—19:00时最大,19:00—22:00时次之,04:00—07:00时最小.     

浅析3G全业务时代电信运营商的竞争与发展

随着电信行业的重组及全业务时代的到来,三大运营商的市场竞争愈演愈烈。3G业务的开展、品牌建设的推进、服务力的提升将是未来运营商竞争与发展的关键。本文从市场经营现状、产品特色发展以及服务体系建设等方面对电信运营商未来发展方向进行了研究,并提出了一些建议。     

面向5G新空口技术的LDPC码标准化研究进展

第5代移动通信系统(5th generation mobile communication system,5G)业务需求的多样性以及各业务场景的典型特性给新空口(new radio,NR)的信道编码技术带来困难及挑战,信道编码方案成为通信业界及学术界的重要研究课题之一。因具备优越性能,低密度奇偶校验(low-density parity-check, LDPC)码被确定为5G增强移动宽带(enhanced mobile broadband, eMBB)场景数据信道编码方案。在对5G及LDPC码进行概述的基础上,阐述了包括码设计、译码算法、速率匹配和性能评估在内的面向NR的LDPC码标准化工作研究内容。此外,对近年来第3代合作伙伴计划(3rd generation partnership project, 3GPP)各成员机构在无线接入网层一(radio access network layer 1, RAN1)标准化工作组历次会议中提出的LDPC码编码方案进行分析和对比,并在此基础上对LDPC码标准化相关工作进行总结。     

伞齿轮精锻模超塑成形热喷涂层的超塑性固相扩散焊接

本文研究了３Ｃｒ２Ｗ８ＶＡ钢伞齿轮精锻模型腔火焰喷涂Ｇ１１２ＷＣ合金层及在模具精密成形时涂层的超塑性扩散焊接。在８２０℃，应变速率ε为３×１０－４ｓ－１的压缩条件下．基材和涂层均发生超塑协调变形，实现稳定可靠的超塑固相连接。研究表明，此时涂层孔洞闭合，涂层颗粒间界及涂层与基材的结合界面完全焊合。超塑性扩散焊接涂层的结合强度与高频和火焰重熔涂层相当，其耐磨性比高频重熔和火焰重熔高２０％～３０％，比改善前３Ｃｒ２Ｗ８ＶＡ模具高达６０％以上。