钌配合物[Ru(phen)2(7-R-dppz)]2+( R=-CH3,-F,-CF3)的合成、表征及DNA键合性质

合成并表征了三个钌(II)多吡啶配合物[Ru(phen)2(7-CH3-dppz)]2+ (i)、[Ru(phen)2(7-F-dppz)]2+ (ii)和 [Ru(phen)2(7-CF3-dppz)]2+ (iii).利用光谱法和粘度实验研究了配合物与DNA的结合行为.结果表明3个配合物均以插入方式与DNA结合,其与DNA的亲和力顺序为(ii) > (i) > (iii).同时采用密度泛函(DFT)理论计算合理解释了3个配合物与DNA结合行为的差异.     

国资重组将带来多少增值?

上海国资委主任杨国雄在7月12日召开的一次内部会议上明确指出,2010年下半年在办好世博会的同时,将积极推进国企改革发展,并突出"5+1"工作。上海国资系统的工作重心将发生转移,这意味着今年下半年上海国资整合将步入快车道。无独有偶,上海国资委7月15日召开的半年度新闻例会上再次强调,将按照年初确定今年资本化率达到30%的目标,全力支持符合条件的企业实现整体上市或核心资产上市,上海国资整合在近期风云再起。     

两种消除Klebsiella pneumoniae重组型质粒的方法

对于工业发酵菌种肺炎克雷伯氏菌(Klebsiella pneumoniae),研究发现有两种消除其重组型质粒的有效方法,一种是连续传代培养,另一种是使用消除剂十二烷基硫酸钠(SDS)。对K.pneumoniae重组菌连续20代传代培养后,发现其质粒具有较高的消除率;而以0.2%SDS复合Ca2+处理K.pneumoniae重组菌,也能有效消除其重组型质粒,且该方法省却了反复的传代培养,能快速得到质粒消除菌,更具简便易操作性。消除了质粒的K.pneumoniae能再次接纳新的质粒,有效避免了因质粒不相容性带来的转化不成功,进而可用作宿主菌积累更多的生理性状。     

Ancient DNA sequences of rice from the low Yangtze reveal significant genotypic divergence

Rice (Oryza sativa) was first domesticated in the lower and middle Yangtze regions of China, and rice remains have been found in many Chinese archaeological sites. Until now, only phenotypic archeobotanical evidence, such as the spikelet bases of ancient grains, has been used to speculate on the domestication process and domestication rate of rice. In this study, we sequenced 4 genomic segments from rice remains in Tianluoshan, a site of the local Hemudu Neolithic culture in the low Yangtze and two other archaeological sites (~2400 and 1200 BC, respectively). We compared our sequences with those of the current domesticated and wild rice (O. rufipogon) populations. At least two genotypes were found in the remains from each site, suggesting a heterozygotic state of the rice seeds. One ancient genotype was not found in the current domesticated population and might have been lost. The rice remains belonged to the japonica group, and most if not all were japonica-type, suggesting that the remains might be at an early stage of indica-japonica divergence or an indica-japonica mixture. We also identified sequences with significant similarity to those from species of Sapindales, Zygophyllales, and Brassicales, which is consistent with the identification of other plant remains in the Tianluoshan site and the common rice field weeds such as mustards in southern China.     

高校图书馆业务流程重组与优化组织结构的思考

本文分析了高校图书馆业务流程重组与优化组织结构的动因和内容,并指出图书馆要实现知识管理,做好知识服务,就需要建立一套与此相关的工作机制及服务机制,改革组织结构,重组业务流程,优化人才使用环境。     

DNA damage,signaling and repair: Protecting genomic integrity and reducing the risk of human disease

DNA is the genetic material that contains the "instructions" to not only guide the continuation of life but also govern the development, metabolism and functioning of living organisms. DNA damage occurs daily due to various exogenous (environ-     

染色质量组试管婴儿

日益发展的体外受精技术正广泛应用于临床不孕症的治疗，但可能造成缺陷基因下传。胚胎着床前遗传诊断技术可早期诊断胚胎遗传缺陷，减少遗传病胎儿的妊娠，但不能修正患儿的基因缺陷。在此，作者提出染色质量组试管婴儿的构想，即在体外受精时剔除有缺陷的染色体，再植入其他个体相应的正常染色体，从而达到修正胎儿遗传缺陷，改善胎儿遗传品质的目的。     

DNA损伤和修复的生物标记物的探讨

某些物理化学因子，如紫外线，电离辐射和化学诱变剂等，都有引起生物突变和致死的作用。这些物理化学因子均能作用于DNA，造成其结构和功能的破坏。熙而在一定条件下，生物泪L体能使其DNA的损伤得到修复。本文主要介绍几种修复系统和生物标记物。     

人类基因组计划的历史回顾

美国《科学》杂志社新闻部编撰了一份有关人类基因组序列探索的历史大事年表 ,从沃森和克里克发现DNA双螺旋结构直到最近人类基因组草图的公开发表     

蓝莓果实总DNA的提取

本文介绍一种提取蓝莓果实基因组DNA的有效方法,即在传统的CTAB法的基础上,加入一定量的PVP。所提取的DNA经0.8%的琼脂糖凝胶电泳,结果证明,提取的DNA片段长度整齐,符合分子生物学后续操作的要求,并且DNA没有被降解。