胞质雄性不育新基因T1243在大白菜中的遗传转化

以大白菜带柄子叶为外植体,通过农杆菌介导法,优化大白菜的遗传转化体系,得出结论:津育75的最佳筛选培养基为MS+2.0mg/L 6-BA+0.3mg/L NAA+7.0mg/L AgNO3+7.5mg/L Kan+200mg/L Amp,外植体在黑暗条件下预培养2d,然后用OD600值为0.1菌液侵染2min,共培养2d时转化率最高,为35.48%.对抗性植株进行分子检测,结果表明T1243基因已整合到大白菜中,并可以正常表达。     

理工科研究生对转基因食品认知态度的再调查

为了进一步研究理工科研究生对转基因食品的认知态度和购买意愿的变化,继2010年12月调查之后,2013年6月随机调查了490名理工科研究生,结果表现出新特征:除了对转基因生物和转基因食品仍旧保持较高程度的认知,共有78.02%的人持"谨慎支持"或"中立"态度,持绝对"支持"或"反对"的比例较小;在价格相当时优先购买传统食品的比例由99.30%下降到77.29%;降价对消费倾向影响较大,当转基因食品便宜40%时总计会有61.12%的人购买该类食品,但仍有11.84%的人表示价格再低也绝不购买转基因食品。分析结果表明:理工科研究生对转基因食品的认识较为理性;专业知识的学习对于转基因生物认知态度有重要影响;多数人对转基因生物持支持或谨慎支持态度;价格因素是影响消费倾向的关键因素。     

大豆增产四措施

1．施用多效唑。多效唑又叫氯丁唑，是一种新型植物生长调节剂。在大豆上施用多效唑，可使植株矮化，茎秆变粗，叶柄缩短，叶片功能期延长，有利于通风透光和防止倒伏，并能兼治大豆花叶病，一般增产20％左右。生产中，一般在大豆分枝到初花期，每667平方米喷施250毫克／千克的多效唑溶液25千克。     

《自然》杂志公布今年值得期待的科技突破

正2014年已经到来,在新年最新一期(1月2日出版)《自然》杂志上,公布了这一年值得期待的研究成果,其中包括转基因猴、干细胞临床实验等。制造出转基因猴包括日本庆应义塾大学的遗传学家佐佐木惠里和干细胞生物学家冈野秀行领导的研究小组在内的几个国际研究小组,均希望在2014年,创造出具有免疫系统缺陷或脑部疾病的转基因灵长类动物。     

当心转基因生物安全的“陷阱”

2008年7月9日，国务院常务会议审议并原则通过了转基因生物新品种培育科技重大专项。该专项的实施“对于增强农业科技自主创新能力，提升我国生物育种水平，促进农业增效和农民增收，提高我国农业国际竞争力，具有重大战略意义”．中国要开发拥有独立自主知识产权的转基因作物。根据《国家中长期科学和技术发展规划纲要》，从现在起到2020年，     

给转基因技术套上管制的缰绳

以转基因技术为代表的现代生物技术突破了传统农业技术的局限。质疑声存在的同时,需阐明转基因技术对我国农业发展的意义,论证现有转基因作物的安全性。     

转人乳基因牛会否突破防线?

近日,中国农业大学李宁院士的科研团队在PlosOne杂志发表研究成果,他的团队将编码人乳中的乳清蛋白、乳铁蛋白、溶菌酶及一些抗体基因转入牛的体细胞核,并用这些体细胞核成功克隆出17头转基因牛,可以生产出含有人乳活性成     

转APX基因烟草抗旱能力研究

用毛白杨中克隆得到的抗坏血酸过氧化物酶(APX)基因,构建植物表达载体pBI121-APX,将pBI121-APX载体用农杆菌介导法转化烟草,成功得到转基因烟.PCR、PCR-Southern的结果表明,APX基因已经成功的整合到转基因烟草基因组中.干旱实验结果表明,与对照相比较,转基因烟草表现出较强的抗旱性.     

Rapid growth and sterility of growth hormone gene transgenic triploid carp

Triploid carp (100%) with 150 (3n=150) chromosomes were obtained by crossing the females of improved tetraploid hybrids (♀, 4n=200) of red crucian carp (♀)×common carp (♂) with the males of diploid yellow river carp (♂, 2n=100). The crosses yielded transgenic triploid carp (positive triploid fish, 44.2% of the progeny) and non-transgenic triploid carp (negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season. Morphologically, both the positive and negative triploid fish were similar. They had a spindle-shaped, laterally compressed, steel grey body with two pairs of barbells. Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents. The positive and negative triploid fish were raised in the same pond for 2 years. The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish. The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish. Thus, we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny.     

Salicylic acid and ethylene signaling pathways are involved in production of rice trypsin proteinase inhibitors induced by the leaf folder Cnaphalocrocis medinalis （Guenee）

The roles of signaling pathways in the production of trypsin proteinase inhibitors (TrypPIs) in rice infested by the leaf folder (LF) Cnaphalocrocis medinalis were studied. Infestation by LF increased TrypPI levels in the leaves of rice plants at the tillering, booting and flowering stages but decreased TrypPI levels at the ripening stage; TrypPI levels in rice stems did not increase at any developmental stage. Infestation by LF at the tillering stage systemically increased TrypPI levels in leaves but not in stems; it also enhanced salicylic acid (SA) levels in leaves and stems, and the ethylene level released from plants. However, LF infestation did not increase JA concentrations. Exogenous application of SA or ethylene enhanced TrypPI levels in the leaves and stems of plants at the tillering stage, whereas treatment with both SA and ethylene induced lower levels of TrypPIs than treatment with SA or ethylene alone, suggesting an antagonistic effect of SA and ethylene on TrypPIs induction. The results suggest that both SA and ethylene signaling pathways are involved in the production of TrypPIs in rice induced by LF; moreover, the antagonistic effect of SA and ethylene may explain the changes in TrypPI levels seen at different plant developmental stages and in different organs.