哺乳动物植入前胚胎发育的程序化

哺乳动物植入前胚胎发育的程序化过程,是发育生物学的核心问题,弄清这一过程,对研究哺乳动物早期发育有着重要意义.因受研究方法、实验材料等因素的影响,该研究领域的进展缓慢.文章就近年来在植入前胚胎发育的分子事件、母源调控向合子型调控的过渡、细胞核重建、染色质的重建机制、基因组甲基化与基因表达等方面的研究进展进行了综述.     

基于IEC 61850的程序化变电站在深圳电网中的应用

介绍了IEC61850标准及其在程序化变电站中的应用与推广,IEC61850标准为程序化变电站的实现提供了平台.对变电站程序化操作的基本情况,程序化操作对一次设备和二次设备的要求进行了分析,结合深圳供电局110kV莲花山变电站改造项目中程序化操作的实施,研究了变电程序化现场实施的系统构成、技术基础等,提出程序化操作将是变电站自动化系统发展的趋势.     

浅议工程监理控制工作

本文从工程监理的中心任务切入,论述了实现监理目标必须做好工程监理控制工作,有效地实施监理控制必须做到工作程序化、管理标准化、控制科学化。     

人胎儿中枢神经系统发育中的程序性细胞死亡

用流式细胞术结合原位末端标记技术研究了１２￣３９周人胎儿神经系统发育中程序性细胞死亡（ＰＣＤ）的发生和变化规律。观察到各胎龄段胎儿的各代表性脑区均有ＰＣＤ发生,可见两次ＰＣＤ峰值分别出现在第１２周和第３９周。推测第１次细胞凋亡高峰可能与清除过多的神经细胞有关,第２次可能与建立精确的神经回路有关。     

选择死亡：细胞自杀之谜

非洲原始丛林里,一头老象步履沉重地孤独前行。它历尽沧桑,气数将尽,在一种神秘力量的驱动下,去寻找它祖祖辈辈安息的地方;大西洋海岸,数百头巨鲸一次次冲向海滩,面对养育自己的海洋,义无反顾地急切地结束生命,任何力量也改变不了它们的决定;一头身患不治之症的鼹鼠,一头冲进家庭成员方便的地方,并呆在这臭气熏天的地方再也不肯出来,直到咽气;过了繁殖期的成年蝴蝶,冲向大地,拼命地扑打自己美丽的翅膀,直到精疲力竭而死;一头7岁半的雄性黑猩猩,悲痛欲绝地独自守在去世的母亲身边,一步也不肯离开,它拒绝进食,最后死去…     

紫薯花青素的成分鉴定及其抗白血病潜在靶点的筛选验证

为探讨紫薯花青素(Purple Sweet Potato Anthocyanin, PSPA)在急性淋巴细胞白血病(Acute Lymphoblastic Leukemia, ALL)防治中的靶点,本文提取、纯化并鉴定了紫薯花青素,基于分子对接技术预测了紫薯花青素的潜在靶点。结果显示：从川紫4号紫薯中鉴定出25种花青素,分别是矢车菊素、芍药色素、天竺葵色素及其衍生物,各种成分的相对含量为0.1%-23.0%。其中,矢车菊素3-阿魏酸-对羟基苯甲酰槐糖苷-5-葡萄糖苷(Cyanidin 3-feruloyl-p-hydroxybenzoyl sophoroside-5-glucoside)相对含量最高,达到22.63%。基于矢车菊素抗氧化活性较其他两种花青素更强、二乙酰化的花青素更稳定的特点,该化合物被选择作为后续分子对接的配体,分别与程序性细胞死亡(Programmed Cell Death, PCD),如凋亡、焦亡、坏死性凋亡、铁死亡及铜死亡相关的关键蛋白进行分子对接分析。结果表明,紫薯花青素可以结合到抗凋亡蛋白(Bcl-2)、E3泛素连接酶(Parkin)蛋白、胱氨酸转运蛋白(SL...     

纯0—1问题的一个程序化算法

在整数规划分支定界解法的基础上,考虑到纯０－１问题变量的特点建立了其标准型,改进了分支和定界的方法和过程,得到了一个快速终止程序化算法。     

Phytophthora elicitor PB90 induced apoptosis in suspension cultures of tobacco

The protein elicitor PB90 secreted by Phytophthora boehmeriae is an efficient elicitor inducing the hypersensitive response and systemic acquired resistance in tobacco plants. Here, we observed cell death in suspension-cultured cells of Nicotiana tabacum BY-2 with PB90 treatment using Trypan blue staining method. And this cell death could be suppressed by cycloheximide, an inhibitor of proteins synthesis, which implies that PB90-induced celldeath was an active cell death process requiring new protein synthesis. DAPI staining revealed that PB90 induce rapid chromatin condensation, margination, apoptotic bodies‘ formation and DNA laddering, further TUNEL assay also observed the specific breakage of 3 ‘-OH ends. All of the above common morphological characteristics indicated that PB90 induced apoptosis in suspension cultures of tobacco, suggesting that hypersensitive response induced by PB90 is an apoptotic process.     

光动力疗法诱导的免疫原性细胞死亡的研究进展

光动力疗法（PDT）已被证明可以诱导免疫原性细胞死亡（ICD）.但是由于PDT本身的局限性以及肿瘤部位的免疫原性差和免疫抑制环境的原因,单独的PDT在癌症免疫治疗中难以获得强大而持久的适应性抗肿瘤功效,往往需要采用不同的策略来提高肿瘤的免疫应答效果.因此,文章简述了光动力学机理、ICD原理,以及对PDT诱导的ICD的各种不同策略进行了总结和讨论,策略包括增加氧气（O₂）和靶向性提高PDT效果,PDT联合光热疗法（PTT）和化疗等治疗方式,PDT结合免疫检查点以及免疫佐剂抑制来提高ICD效应,以此为研究人员提供更多的癌症免疫治疗方面的参考.     

Difference in apoptosis-associated genes expression profiling and immunohistology analysis between Kashin-Beck disease and primary osteoarthritis

Kashin-Beck disease(KBD)is a chronic and deformed endemic osteoarthritis,without fully known etiology.As compared to primary osteoarthritis(POA),its pathogenesis still exists controversial.We performed this study to discriminate the difference in genes expression involved in apoptosis from KBD cartilages and POA cartilages.Microarray analysis and Ingenuity Pathway Analysis(IPA)were used to identify the molecular mechanisms/canonical pathways implicated in KBD arthritis.Immunohistochemistry staining was carried out to detect tissue distribution of PDCD5 and EGR1 in the knee cartilages from KBD and POA.The 23 up-regulated apoptosis-related genes with[2-fold change were identified.‘‘Role of Macrophages,Fibroblasts,and Endothelial Cells in Rheumatoid Arthritis’’signaling was screened as the most relevant pathway through IPA.The 8 differentially regulated genes were verified by qRT-PCR analysis.Programmed cell death 5 in middle and deeper zones and early growth response 1 in superficial and middle zones showed protein overexpressed in KBD cartilage samples comparing to those in POA cartilage samples.Data indicates a higher expression level of apoptosis-related functional genes in KBD articular cartilage compared with POA articular cartilage.