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WEI Wu DING Guohui WANG Xiaojing SUN dingchun TU Kang HAO Pei WANG Chuan CAO Zhiwei SHI Tieliu LI Yixue 《科学通报(英文版)》2006,51(10):1199-1209
The recent outbreak of Streptococcus suis in some districts of Sichuan Province in China has caused over 30 deaths and more than 200 infections in human be-ings[1]. Streptococcus suis is a Gram-positive, faculta-tively anaerobic bacterium, a peculiar path… 相似文献
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WANG FengGe ZHAO JiuRan DAI JingRui YI HongMei KUANG Meng SUN YanMei YU XinYan GUO JingLun WANG Lu 《科学通报(英文版)》2007,52(2):215-223
In the current study, 1900 maize simple sequence repeat (SSR) primers published in MaizeGDB were screened utilizing reference literature, 15 representative Chinese maize inbred lines and 15 Chinese maize hybrids from national regional testing. In total, 500 highly polymorphic primers were identified and used to construct a genetic map. 100 evenly distributed primers, 10 primers per chromosome, were further selected as a set of universal SSR core primers, recommended as preferred primers for general studies. These core primers were then redesigned and used to construct a high throughput multiplex PCR system based on a five-color fluorescence capillary detection system. We report here that two sets of ten-plex PCR combinations have been constructed, each consisting of 10 primers, with one primer per chromosome. 相似文献
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黄颡鱼红头病病原研究 总被引:2,自引:0,他引:2
郑善坚 《浙江海洋学院学报(自然科学版)》2009,28(4):406-409
从患红头病的黄颡鱼的肝脏中分离获得HS807菌株,经人工感染试验为该病的致病菌,结合形态观察、培养特性及16S rRNA基因序列分析,HS807菌为鲇鱼爱德华氏菌。药敏试验表明,对复方新诺明、环丙沙星、先锋Ⅳ、强力霉素、青霉素、依诺沙星、环丙沙星、菌必治、新霉素、呋喃唑酮等药物敏感。 相似文献
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参考果蝇、卤虫与锯缘青蟹的线粒体DNA 16SrRNA基因序列进行了日本绒螯蟹相同基因片段的引物设计、PCR扩增及序列测定,得到567bp的咸基序列,其A、T、G、C含量分别为194bp(34.22%)、216bp(38.10%)、98bp(17.28%)59bp(10.41%),与果蝇、卤虫及锯缘青蟹类的16SrRNA基因片序列含量相似。 相似文献
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根据桑(Morus bombycis)泛素基因编码区的序列(DQ839402.)设计特异引物,用RT - PCR方法克隆桑泛素基因的编码区.序列分析表明,该编码区长240 bp,编码79个氨基酸,软件分析推测编码蛋白的相对分子量和等电点分别为8.74 kD和7.16.通过同源建模获得了桑泛素基因编码蛋白的理论三维结构.将桑泛素基因与pET - 32a(+)连接,构建原核表达载体pET - 32a - ub,经IPTG诱导,在大肠杆菌BL21(DE3) 中获得高效表达,并经western bolt 印迹证明实现了原核表达,为进一步研究其作用机理奠定基础. 相似文献
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Magnetotactic bacteria (MTB) are of interest in microbiology, biomineralization, advanced magnetic materials, and bio-geo-sciences because of their ability to form highly ordered intracellular magnetic minerals. Great strides for MTB studies have been made in the past four decades. In this paper we complied the first internet-accessible database for MTB, Database of Magnestotactic Bacteria (DMTB). It contains information of 16S rRNA gene sequences, corresponding ecological metadata, oligonucleotides, and magnetic properties of MTB. The comprehensive information contained in DMTB will provide a very useful data resource for researchers from different disciplines. The website of DMTB is at http://database.biomnsl.com/. 相似文献
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Jean-Louis Reymond 《复旦学报(自然科学版)》2005,44(5):837-837
1 Introduction In de novo protein design one attempts to create artificial proteins with defined structure and function from first principles, usually with the help of trial-and-error procedures that scan a large number of possible amino acid sequences. Our approach to de novo protein design is based on peptide dendrimers. Dendrimers are tree-like structures that adopt a globular or disk-shaped structure as a consequence of topology rather than folding. Our peptide dendrimers are obtained by a… 相似文献