鲤鱼育种研究

系统地介绍了迄今为止鲤鱼育种已采用过的方法和已取得的成就,特别着重介绍了鲤鱼的育成品种以及一些新技术在鲁鱼育种的应用,并对今后的发展做了展望。     

草鱼头肾淋巴细胞体外转化培养影响因素的研究

从草鱼（Ctenopharyngoden idellus）头肾中分离淋巴细胞,利用DMEM培养液,采用MTT比色法,测定草鱼头肾淋巴细胞在体外受特异有丝分裂原植物凝集素(PHA）刺激下发生的转化率,研究在细胞培养过程中不同因素对淋巴细胞转化率的影响,以期建立一种较可靠的鱼类免疫学研究技术,实验表明,头肾淋巴细胞体外增殖受PHA浓度、小牛血清浓度及温度条件影响,PHA的最佳质量浓度为80mg／L,小牛血清的最佳体积分数为15％,最适温度范围是28－30℃,实验结果还表明,采用MTT比色法测定鱼类淋巴细胞体外转化率是一个既方便又可靠的方法。     

Simultaneous estimation of genetic linkage and preferential pairing factor for a triploid population with unphased markers

Triploids,recognized to occur more frequently in natural and experimental populations of many species than previously appreciated,display important economic and biological values.Despite this,however,linkage analysis for triploids has not been well explored.We develop a statistical model for estimating and testing the linkage between molecular markers in a triploid population derived from a tetraploid and diploid parent.The model incorporates one important meiotic feature of tetraploids by which more homologous chromosomes pair with a greater likelihood than less homologous chromosomes.By implementing the EM algorithm within the maximum likelihood framework,the model provides a procedure for simultaneous estimation of the linkage and preferential pairing factor.The model accommodates the segregating patterns of pseudotest markers and intercross markers with different amounts of informativeness.The utility of the model was validated through a real data analysis and simulation studies.The model provides a statistical tool for linkage analysis in a triploid population by taking into account meiotic behavior of tetraploids.Results from the model will help to shed light on the genetic diversity and origin of a polyploid population.     

A quantitative interpretation of the metabolism-size relationship in animals

Summary Experimental studies with carp supported our hypothesis, that the regular decrease in the mass-specific rate of resting metabolism with increasing body mass may be explained principally by a combination of a decrease in the mass-specific rate of tissue metabolism, and an increase in the relative size of organs and parts of low metabolic activity with increasing body mass.     

三倍体无籽西瓜的组织培养

将三倍体无籽西瓜带子叶幼芽以及成株顶芽和腋芽培养在附加ＢＡ２ｍｇ·Ｌ－１的ＭＳ培养基中，芽体均可以扩增为芽丛．并首次报道了花芽在培养基中展开后子房愈伤组织也可以再生不定芽．不同途径得到的幼芽进一步扩增或转移到ＭＳ附加ＩＡＡ０．２ｍｇ·Ｌ－１的培养基后，均可以分化不定根，从而形成完整植株．     

草鱼幼鱼对不同缩结定置网片反应特性初探

初步研究了草鱼幼鱼对三种缩结定置网片的反应特性。结果表明;缩结对草鱼幼鱼在水槽内的分布特征没有显著影响,即无论在水槽内放置哪种网片,草鱼幼鱼均喜欢在网片邻近区域长时间停留,趋网、碰网尾次数及碰趋比也不随缩结不同而发生明显变化,但缩结对穿网尾次数、穿趋比和穿碰比有显著影响,扁形网目阻拦作用最大,长形网目次之,方形网目最小,推测穿网尾次数、穿趋比及穿碰比均与网目高度、鱼体高度之差和网目宽度、鱼体宽度之差的乘积成正比。     

RESEARCH ON FIBER MORPHOLOGY AND PULPING PROPERTIES OF THE TRIPLOID OF PLPULUS TOMENTOSA FROM PLANTATION AT DIFFERENT AGES

INTRODUCTIONThe populus tomentosa laboratory of Beijing ForestryUniversity Chinese led by professor Zhu zhiti hassuccessfully cultivated the triploid of populustomentosa which is a fast growing cellulose materialbearing international leading level. The triploid ofpopulus tomentosa has got a popularization in northChina for its excellent features of fast growing,anti-disease and highly active. We have carriedthrough the detailed research on its biology structure, lignin distribution in c…     

萍乡肉红鲫肠道三种内分泌细胞的免疫组化定位分析

采用免疫组织化学方法,对萍乡肉红鲫肠道中分泌胃泌素（Gastrin）、生长抑素（ss）及5-羟色胺（5’-HT）等3种内分泌细胞的分布情况进行了研究。结果显示,3种内分泌细胞中,除未发现分泌5-羟色胺的细胞存在外,分泌胃泌素与生长抑素的细胞在鱼体的整个肠道皆有分布,且其分布密度从前肠向后肠方向逐渐降低。分析表明,萍乡肉红鲫肠道中的这3种内分泌细胞的分布规律与其他鱼体的分布情况基本一致。     

不同处理方式对鲢鱼鱼肉蛋白乳化性的影响

以鲢鱼鱼肉为原料,研究了超声波、微波、加热处理对鲢鱼鱼肉蛋白溶解性的影响.结果表明:蛋白的乳化性及乳化稳定性随加热温度的升高、微波和超声功率的增大及时间的延长均呈现先增大后减小的趋势.与室温下的蛋白相比,35℃加热9 min时的蛋白乳化性提高了36.79%,乳化稳定性在7 min、35℃时提高了14.2%;与未经微波处理的蛋白相比,微波功率100 W、40s时,乳化性提高了1.35倍,乳化稳定性在100 W、30 s时提高了15.85%;与未经超声处理的蛋白相比,乳化性在超声功率160 W,超声时间10 min时提高了56.7%,乳化稳定性在200 W、15 min时提高了9.85%.     

实验红鲫标准化研究与应用的现状及展望

摘要: 实验红鲫具有体型大小适当、成熟早、繁殖力强、体外产卵发育、体色全红、杂食性等优点。本世纪初以来,开展了实验红鲫驯化、品( 种) 系培育、生物学特性、地方标准等研究。实验红鲫已广泛应用于鱼类育种、毒理学、遗传学、生理学、水生态环境监测等。