Expression of lysine-rich protein gene and analysis of lysine content in transgenic wheat

Expression vector pBPC102, which carries winged bean lysine-rich protein (wblrp) gene and dihydropicolinate synthase (DHDPS) gene, was transferred into hexaploid winter wheat cv. Jinghua No.l, Jing411, You899 and Yangnongl5 explants of immature inflorescence and immature embryos by particle bombardment. More than 100 transgenic plants were obtained under the selection of s-(2-aminoethyl)-L-cysteine (AEC). Confirmed transgenic plants of To and TI generation by PCR and PCR-Southern blotting analyses showed successful integration of wblrp gene into wheat genome. Analysis of transgenic plant lines of T2 by Northern dot-blotting showed good expression of wblrp gene in offspring seed. The content of free lysine in leaves, contents of bound lysine and total proteins in seeds of T2 transgenie wheat lines were determined and analyzed. Among 34 tested transgenic lines, levels of free lysine content in leaves of 9 transgenic lines are 2~3times higher than un-trans-formed wild-type cultivars. Among 17 analyzed transgenic lines, bound lysine content of 4 transgenic lines is more than 10% higher than that of wild-type cultivars. Our research suggests that introducing wblrp gene into wheat is an effective way to improve its nutrition quality.     

小麦新品种"54368"生育的农业气象条件试验总结

用鲁麦1号小麦作对照,通过分期播种的方法,对小麦新品种"54368"进行了田间试验.结果显示,54368小麦需要的热量条件少于鲁麦1号,生育期比鲁麦1号短2天左右,抗蚜虫能力比鲁麦1号略差,抗锈病能力与鲁麦1号相当,丰产性能明显优于鲁麦1号.     

金佛山岩溶生态系统初步探讨--岩溶泉水化学特征分析

从岩溶水化学性质方面对金佛山岩溶生态系统作了初步的分析,选取了两个具有代表性的岩溶泉进行对比性加密观测,结果表明：不同海拔高度出露的岩溶泉水化学性质和岩溶强度存在较大不同;具有不同海拔高度的小鲵泉和双龙泉水化学性质随时间变化的稳定性出现较大差异。     

暗管排降对小麦养 分、干物质、籽粒产量及品质的影响

着重探讨了南方不同生态类型圩区农田埋设塑料暗管对小麦地上部分矿质营养吸收、干物质累积和分配、产量及品质的影响。试验结果表明：农田埋设塑料暗管排水能显著提高麦株地上部分对Ｎ、Ｐ,Ｋ矿质营养元素的吸收,促进小麦各生育期尤其是拔节孕穗期地上部分干物质累积,小麦增产幅度达到２０．３％￣５３．３％,并能提高籽粒蛋白质和氨基酸含量,改善小麦籽粒的品质。     

水分胁迫条件下化控物质浸种对小麦根苗生长的影响

采用水培溶液添加PEG6000模拟干旱胁迫的方式，研究了化学调控物质浸种对不同程度水分胁迫下小麦根系及幼苗生长影响。结果表明，水分胁迫不同程度地降低了小麦幼苗的株高、叶面积、地上部及根的干重、根系活力、SOD、POD活性等，并明显增加了根系MDA含量水平。化学调控物质浸种处理可以增加各种条件下小麦的总根长、叶面积、根活力及SOD与POD活性，且表现出在干旱胁迫下增加幅度高于正常处理的现象。化学调控物质浸种有利于缓解干旱胁迫带来的不良影响。利用FA、GA、MET及稀土等化学调控物质浸种具有促进根系生长、培育小麦壮苗的突出作用。图3，表2，参14。     

半主动空气弹簧悬挂系统控制策略及仿真分析

随着铁路不断提速，对车辆运行品质的要求也越来越高。半主动控制技术在机车车辆上的应用为进一步提高机车车辆动力学性能进而提高其运行品质开辟了新的途径。包括空气弹簧的二自由度悬挂系统的控制策略进行分析，结合控制策略提出了一套以调节空气弹簧节流孔直径从而改变悬挂系统阻尼为目的的半主动控制方案，运用计算机软件SIMULINK对半主动控制空气弹簧悬挂系统进行了仿真。研究结果表明，运用半主动控制技术可以得到更好的空气弹簧悬挂参数，提高整个悬挂系统的减振性能以改善车辆运行平稳性。     

黑麦KingⅡ中白粉病抗性基因的染色体定位

寻找新的白粉病抗源对促进小麦白粉病抗性育种有重要意义.黑麦是重要的白粉病抗源之一,加强对黑麦白粉病抗性调查的研究也将有利于小麦育种.本实验调查了黑麦KingⅡ及衍生自中国春×黑麦KingⅡ的一套小麦一黑麦附加系的白粉病抗性.结果表明,黑麦KingⅡ对白粉病免疫,附加系中除3R和6R附加系对白粉病表现免疫外,其余5个附加系都高感白粉病.该结果表明3R和6R染色体上的白粉病抗性基因可以在小麦背景中得到表达.这为将黑麦白粉病抗性基因导入小麦提供了直接的桥梁材料.     

超滤分离纯化麦胚蛋白酶解物的研究

采用超滤法从麦胚蛋白酶解物中初步分离纯化抗氧化活性肽,考察了操作压力、操作温度及料液浓度等工艺参数对超滤过程中膜通量的影响,并对超滤前后麦胚蛋白酶解物的相对分子质量分布、氨基酸组成及抗氧化活性进行了比较.结果表明:采用截留相对分子质量3000的聚砜膜对麦胚蛋白酶解物进行超滤分离的最适工艺条件为操作压力0.30 MPa、操作温度20 ℃、麦胚蛋白酶解物浓度35 g/L;超滤有效地除去了料液中相对分子质量较大的组分,麦胚蛋白酶解物中相对分子质量1 000～500及500～130的组分分别为23.81%、48.63%,其抗氧化活性得到提高,清除O2-·的IC50由超滤前的1.64 mg·mL-1降至超滤后的1.29 mg·mL-1.图5,表3,参13.     

Biomineralization of phototrophic microbes in silica-enriched hot springs in South China

Microbial mats in two hot springs in South China were sampled for the research of mineralization of microbes and its mechanism by the methods of geology and modern biology. The results show that hot spring microbes have the key capability for enrichment of Si, Al, Fe, Ca and other elements, and the microbes are also crucial for the formation of SiO2, CaCO3, clay and so on. The extracellular polymeric substances (EPS) play important roles in the process of mineralization of hot spring microbes, which mainly takes place in the layer of EPS outside cell wall or sheath of cyanobacteria. The sheath outside cell wall, which keeps the normal metabolism of cyanobacteria during the process of mineralization on its surface, is also considerable for the biomineralization of cyanobacteria. According to structure and mineralization characteristics of two microbial mats, the process of mineralization can be divided into three stages, namely, early surface mineralization, middle degradation mineralization, and late des- quamation of mineral. The above conclusions are significant for comprehension of the process of mineralization, the process of deposition and the preservation of microfossil in modern and ancient extreme environments.