雷帕霉素的免疫药理学（综述）

雷帕霉素是一种高效低毒的大环内酯类免疫抑制药,它在细胞外的受体为FK506结合蛋白FKBP－12,胞浆内的激酶mTOR是雷帕霉素已知的主要靶体,它是mRNA翻译的启动调控因子,Kipl是一种周期素依赖性激酶的抑制剂,能降低活化的T细胞内G1期的周期素与其依赖性激酶复合物的水平,mTOR对p70 s6k的活性,真核启努因子4E依赖性蛋白合成及Kip1的下调所需调节信号具有诱变作用,它与FKBP12与雷帕霉素复合物的相互作用干扰了它的功能,雷帕霉素因素阻断了Kipl的下调而将细胞阻滞于G1期。     

Abnormal protein aggregation and neurodegenerative diseases

Abnormal protein aggregation or amyloid is the major cause of many neurodegenerative disorders. The present review focuses on the correlation between sequence and structure features of proteins related to the diseases and abnormal protein aggregation. Recent progress has improved our knowledge on understanding the mechanism of amyloid formation. We suggest a nucleation model for ordered protein aggregation, which can also explain pathogenesis mechanisms of these neurodegenerative diseases in vivo.     

Recent developments of the stoichiometric displacement model for separation processes

Substantial attention has been paid to the stoichiometric displacement models for solute retention (SDM-R) in liquid chromatography and adsorption (SDM-A) at surfaces since they were developed. The SDM has a strongly theoretical basis and has evolved to the point that it is widely applied in chemistry, biochemistry, molecular biology and biotechnology. This review introduces the history, recent developments, and new concepts relating to the SDM, including theory, mathematical expressions, and applications across a broad range of fields.     

利用噬菌体展示筛选结合水稻条叶枯病毒S蛋白的短肽

利用噬菌体展示技术在随机7肽库中筛选到结合水稻条叶枯病毒（rice stripe virus ,RSV）病害特异蛋白（stripe disease-specific protein,S蛋白）的6个短肽,并进行了序列测定,ELISA结果表明,6个短肽和S蛋白具有一定的亲和能力,筛选到的短肽对今后S蛋白功能分析、转基因抗RSV和水稻条叶枯病的诊断提供了条件。     

蛋白质二级结构由氨基酸和mRNA序列编码

据氨基酸序列,密码子序列和蛋白质二级结构序列的比较,指出约18％的两肽的密切子具有反常的蛋白质结构偏好性,并且这种以常不能用随机涨落解释,因而关于蛋白质折迭的Anfinsen原理可能需要作适当修正。     

人胎盘G-蛋白Rab3a cDNA的克隆

为了获取全长的小分子G-蛋白Rab3a,以用于研究Rab3a与其他蛋白相互作用关系,本实验以人胎盘总cDNA为模板,PCR扩增到人Rab3a cDNA全编码区。产物回收后克隆于质粒pYESTrp2的BamHI/XhoI位点,测序结果表明,本实验获得的Rab3a cDNA包含了起始和终止密码子。与PCR引物设计的参照Rab3a比较有5个核苷酸变异,与翻译的氨基酸序列完全一致。由此表明本实验获得的Rab3a cDNA可用于进一步研究。     

蛋白质折叠的计算机模拟

介绍了计算机模拟蛋白质折叠问题的背景、模型和意义。在对模型问题采用Ｍｏｎｔｅ－Ｃａｒｌｏ方法和单纯遗传算法得到能量最小构象的基础上,提出了适用的混合遗传算法,并通过计算机模拟试验对三种方法作了比较。     

蓖麻饼提取蛋白质的研究

本文就蓖麻籽榨油后所剩的物质-蓖麻饼中提取分离蛋白质进行了研究,采用正交设计方法,进行提取分离蛋白质的工艺实验,得到了蛋白质提取率在67.5%-93.1%的较佳工艺条件。     

Effect of the control proliferation of astrocyte on the formation of glial scars by antisenseGFAP retrovirus

Astrocytes play an important role in the formation of glial scars. In order to investigate the effect of inhibitingGFAP gene expression on normal, reactive astrocytes and on glial scar formation, the efficiency of the recombinant antisenseGFAP retrovirus (PLBskG) on the growth, cell cycle, morphology andGFAP gene expression of astrocytesin vitro and on the formation of glial scarsin vivo has been studied by cell growth curves, flow cytometry, immunocytochemistry,in situ hybridization, RT-PCR and Southern blot. The results confirm the recombinant retrovirus (PLBskG) produced growth suppression and G1 arrest of the normal and injured astrocytes. The infected cells become round or ellipoid. The cell processes become fine or retracted. The intensity of staining ofGFAP is reduced. Expression ofGFAP mRNA is down regulated. However, in the control experiment, no obvious effects on the morphology or synthesis ofGFAP on cultured normal and scratched astrocytes infected by primary retrovirus vector (PLXSN) have been observed. The supernatant of PLBskG has been injected into an injured site by microinjectionin vivo. The number and process lengths of GFAP positive cells are obviously reduced around the injured site. The formation of the glial scar is inhibited, showing that the recombinant antisenseGFAP retrovirus can effectively inhibit the growth andGFAP expression of normal and injured astrocytesin vitro and the formation of glial scarin vivo. It is suggested thatGFAP plays an important role in glial scar formation.     

Heat-shock protein 90 inCandida albicans

Researches on Candidal heat-shock protein 90 (HSP90) in recent years are summarized.Candida albicans is a commensal pathogen in human and animals. In immunocompromised individuals it behaves as an opportunist pathogen, giving rise to superficial or systemic infections. Systemic candidosis is a common cause of death among immunocompromised and debilitated patients, in which the mortality is as high as 70%. HSP90 is now recognized as an immunodominant antigen inC. albicans and plays a key role in systemic candidosis as a molecular chaperone. The 47-ku peptide is the breakdown product of HSP90. Patients who has recovered from systemic candidosis produce high titre of antibodies to 47-ku antigen, whereas the fatal cases have little antibody or falling titres. The three commonest epitopes of candidal HSP90 have been mapped, epitopes C, B and H. Epitopes C and H are immunogenic. The antibody probes of both epitopes may be developed into a new serological test agents for systemic candidosis due to rather high specificity and sensitivity. The recent results establish HSP90 as an ATP-dependent chaperone that is involved in the folding of cell regulatory proteins and in the refolding of stress-denatured polypeptides. Some researches on fungal HSP90 and the treatment of patients with candidosis are reviewed as well.