苏铁蕨多糖的提取及其组分分析

采用低温-微波工艺提取苏铁蕨[Brainia insignis（Hook．）J．Sm．]的木质根状茎部分,以95％乙醇、水为溶剂分步提取,获苏铁蕨粗多糖,经纯化为精制多糖．以凝胶过滤色谱法（GFC）测定苏铁蕨多糖的数量及分子量．结果：苏铁蕨中含1种多糖,其数均分子量为31514g·mol^-1,重均分子量为35034g·mol^-1;多糖经酸解成单糖并衍生为乙酰化糖肟,对照乙酰化的标样采用气相色谱法（GC）测定其中含5种单糖组分,其相对含量：葡萄糖88．816％;半乳糖4．631％;阿拉伯糖3．314％;木糖2．085％;甘露糖1．154％．     

硒多糖的研究进展

综述近几年国内外在硒多糖测定方法、提取、药理作用及临床应用等方面的最新研究成果,主要在硒多糖的抗氧化、抗癌抗肿瘤等药理作用及临床应用方面作了系统的阐述.在此基础上讨论了硒多糖需要进一步研究的问题.为硒多糖的进一步开发提供了文献依据.     

不锈钢表面生物膜电化学性质与稳定性

以天然水体为挂膜介质,在304号不锈钢表面培养生物膜.以多糖、脂磷为生物量表征参数,研究开路电位和不锈钢表面生物膜生物量的相关性,并使用扫描电镜表征生物膜形貌.此外,利用紫外线处理生物膜样品.结果表明,在一定开路电位范围内,不锈钢表面生物膜生物量与开路电位具有很好的拟合关系,开路电位可以作为生物量的检测手段.不锈钢表面生物膜具有一定的抗剥离能力,一旦形成将难以剥离.     

川西獐牙菜多糖的提取及含量测定

对川西獐牙菜中多糖进行提取及含量测定;通过正交实验确定提取多糖的优化条件,用分光光度法测定多糖的含量;川西獐牙菜多糖提取的最佳条件是:料液比为1:10、提取时间为2.0h、提取次数为4次、温度90℃.并测得川西獐牙菜中多糖含量为41.60mg/g,回收率为99%,RSD=1.2%结论该方法简便,快速,准确,适用于药材中多糖的含量测定.     

Biological activities of a neutral water-soluble agar polysaccharide prepared by agarase degradation

Depolymerization of agar was performed using agarase, which was extracted from the cell-free medium of a culture of marine bacterial Alterornonas sp. nov. SY 37-12. After ethanol fractionation and lyophilization, the water-soluble agar polysaccharide （WSAP3） was collected. The anti-tumor activity of the product was determined by using Sarcoma 180 tumor in mouse. The tumor inhibition rate of WSAP3 the product was determined by using Sarcoma 180 tumor in mouse. The tumor inhibition rate of WSAP3 reached 48.7% at a dose of 64mg kg^-1 after 15 days treatment. WSAP3 enhanced the aetivities of superoxide dismutase and catalase, which suggests that WSAP3 was effective in promoting the antioxidation ability and eliminating danger from free radicals. The result of flow cytometry showed that the WSAP3 had no activities of cell cycle inhibition or apoptosis-inducing activities. The anti-oxidation of WSAP3 was further confirmed by test in vitro, which might play an important role in anti-tumor activity. The immunological regulation of WSAP3, especially its effect on the phagocytosis ratio and phagocytosis index of rophage was also assayed in test in vivo.