苏云金杆菌H3-111菌株对蓑蛾的毒效

通过苏云金杆菌H3- 1 1 1菌株摇瓶培养液对蓑蛾进行室内毒力测定 ,4 8h毒力回归方程为y=4 1 94 0 +3 31 85x ,r =0 95 31 ,LC5 0 =0 0 0 0 0 2 5 7,与标准品比较其生物效价为 4 86 2 93IU·mg- 1 ,有较高的毒效 ;同时经大田试验表明BtH3- 1 1 1菌株防治蓑蛾高峰期在药后 72～ 96h ,稀释 5 0 0～1 0 0 0倍的防效达 92 4 6 %～ 95 1 5 % ,超过化学农药DDVP的最高防效 (85 0 7% ) ,可替代化学农药用于蓑蛾的防治中     

漏斗状侧耳纤维素酶高活性变株的选育与产酶条件和酶特性研究

以漏斗状侧耳5·01为出发菌株,用NTG和UV依次对孢子、单核菌丝和双核菌丝进行处理,获得了微晶纤维素酶活和羧甲基纤维素酶活分别是出发菌株2.86倍和2.51倍的突变株HCA15。正交试验结果表明:该变株在含有棉籽壳粉、尿素、麸皮、纤维二糖的培养基中酶活高。产酶的最适pH为5.6,最适温度为28℃。酶作用的最适pH为4.6,最适温度为40℃。酶在pH5.0时最稳定,在50℃以上不稳定。     

Mapping and characterization of a tiller-spreading mutant<Emphasis Type="Italic">lazy-2</Emphasis> in rice

Tiller angle of rice is an important agronomic trait that contributes to breed new varieties with ideal architecture. In this study, we report mapping and characterization of a rice mutant defective in tiller angle. At the seedling stage, the newly developed tillers of the mutant plants grow with a large angle that leads to a “lazy“ phenotype at the mature stage. Genetic analysis indicates that this tillerspreading phenotype is controlled by one recessive gene that is allelic to a reported mutant la. Therefore, the mutant was named la-2 and la renamed la-1. To map and clone LA, we constructed a large mapping population. Genetic linkage analysis showed that the LA gene is located between 2 SSR markers RM202 and RM229. By using the 6 newly-developed molecular markers, the LA gene was placed within a 0.4 cM interval on chromosome 11, allowing us to clone LA and study the mechanism that controls rice tiller angle at the molecular level.     

Morphology and mapping analysis of rice (Oryza sativa L.) clustered spikelets( Cl) mutant

The rice clustered spikelets (Cl) mutant exhibits a phenotype that most of branch apical have 2-3 spikelets clustered together,SEM (scanning electron microscope )observation suggested that the Cl gene controlled branch apical development,and influenced the terminal spikelets elongation,The spikelet number was reduced in mutant,indicating that Cl may also have an effect on spikelet number,To map Cl locus,two F2 mapping populations derived from the crosses between the Cl and ZhongHua11,and Cl and ZheFu802 were constructed ,respectively,The Cl locus was roughly mapped between two CAPS markers CK0214 and SS0324,A further fine mapping analysis showed that the Cl locus was mapped between makers R0674E and Cl12560,with genetic distances of 0.2 and 2.1 cM,respectively ,Then we found a PAC conting spanning Cl locus,the region was delimited to 196 kb.This results was useful for cloning of the Cl gene,Allelism test demonstrated that Cl was allelic to Cl2 another rice clustered spikelets mutant.     

Characterization and mapping of a lesion mimic mutant in rice （Oryza sativa L.）

A rice initiation-type lesion mimic mutant (lmi) was identified, which was isolated from an indica rice Zhongxian 3037 through γ radiation mutagenesis. Trypan blue staining and sterile culture revealed that the mutant spontaneously developed lesions on the leaves in a developmentally regulated and light-dependent manner. Genetic analysis indicated that the lesion mimic trait was controlled by a single resessive locus. Using public molecular markers and an F2 population derived from lmi and 93-11, we mapped the lmi locus to the short arm of chromosome 8, nearby the centromere, between two SSR markers RM547 and RM331. The genetic distance was 1.2 and 3.2 cM, respectively. Then according to the public rice genomic sequence between the two SSR markers, lmi was further finely tagged by three CAPS markers: C4135-8, C4135-9 and C4135-10. And lmi locus was a co-segregated with marker C4135-10, providing a starting point for lmi gene cloning.     

黑曲霉黄色变株A-2580产耐温酸性蛋白酶发酵条件的优化

经筛选诱变得到一株产生耐温酸性蛋白酶的黑曲霉黄色变株A－2580，对其发酵条件进行了优化：通过单因子试验和正交试验，确定其最适培养基为（g/L）：麸皮21.6、豆饼粉48.4、NH4Cl2、KH2PO40.8，pH4－5，温度30℃的条件下进行振荡培养，发酵72h，酶活可达6700u/ml。     

冬小麦辐射诱变为春性农大94118的选育研究

本文通过对冬小麦品种PH82-2-2辐射诱变处理，在无零下温度条件下，获得了春性突变后代，选育出农大94118品系，试验结果还指出，冬小麦辐射诱变后获得的春性突变体，性状变异幅度大，突变谱宽，供选择的余地大，是春小麦育种的有效途径之一。     

铜绿假单胞杆菌调节基因突变株的分离及特性

铜绿假单胞杆菌ＰＡＯ１经过亚硝基胍诱变获得了弹性蛋白酶缺失的突变株。经弹性蛋白营养琼脂平板法，从１０次独立处理的６００００个菌落中得到７１株该酶缺失的突变株，其突变株对Ｎ－３－氧代己酰－Ｌ－高丝氨酸内酯（ＨＳＬ）诱导物有无反应分成２组。ＰＡＮＯ６７菌株在加入诱导物后能恢复该酶产生，经过菌落形态、生化特征和细胞膜蛋白质成份的分析，除该酶产生缺失外，突变株ＰＡＮＯ６７与亲本菌株ＰＡＯ１相同，结果表明，ＰＡＮＯ６７是弹性蛋白酶操纵子的调节基因发生突变的诱变菌株。     

水稻光敏核不育突变体与其原种的比较研究

本文对水稻光敏核不育突变体农垦５８Ｓ与其原种农垦５８进行了比较研究。结果表明，光敏核不育突变体农垦５８Ｓ与其原种农垦５８在各个生育期都存在明显的差异。农垦５８Ｓ表现出苗期株高、根长、叶绿素含量比农垦５８显著提高；生殖生长期，农垦５８Ｓ对光周期反应敏感，冠层叶片长度、株高、茎杆重和结实率在长日照或短日照处理下存在显著差异，农垦５８在生殖生长期对光周期反应不敏感。根据以上实验结果和已有的文献报道得出结论：水稻光敏核不育突变体农垦５８Ｓ不仅仅是雄性育性受育性转换期光周期调控这一个性状的突变，而且是同时伴随着多种性状的突变。