肿瘤电化学疗法的体外实验

肿瘤电化学疗法的体外实验梁基选，张长弓，苏金华，陈宝　（抗癌中心）肿瘤电化学治疗是根据瑞典放射医学专家Ｎｏｒｄｅｎｓｔｒｏｍ提出的血管－间质闭合电路理论，开展的一项新的临床治疗恶性肿瘤方法。主要是将正负电极分别插入瘤体及周围组织，形成电回路，干扰肿瘤...     

肌酸激酶MM亚型不连续琼脂糖凝胶电泳法的建立

肌酸激酶同工酶(CK)主要分为CK—MM、CK—MB、CK—BB,而CK—MM可以进一步分离为MM_1、MM_2、MM_3等亚型。急性心肌梗塞(AMI)时,CK—MM_3大量入血而升高,特别是MM_3/MM_1的比值升高,对AMI具有极好的特异性和早期诊断的价值。此外,对患者是否使用溶栓疗法具有临床应用意义。我们采用不连续缓冲系统电泳分离CK—MM亚型,方法简便,分辨率好,便于普及推广。     

上消化道病内镜微波组织凝固疗法的临床应用与评价

本文报告应用内镜微波组织凝固疗法(EMCT)治疗胃息肉、胃癌、消化性溃疡三种疾病21例、39次治疗。提出了溃疡面搔刮加微波凝固治疗难治性溃疡、及EMCT联合内镜下局部注射化疗治疗进展期胃癌等方法。胃息肉13例27枚及Ⅱa型早期胃癌1例与消化性溃疡3例全部达到治愈。85.2％的胃息肉EMCT一次治疗成功,Ⅱa型早癌一次成功,消化性溃疡2例一次成功。进展期胃癌4例均达到肿块缩小、狭窄的胃腔扩大与自觉症状改善的效果。鉴于疗效显著,认为三种疾病均适用本疗法;并就EMCT的适应症、使用限度和治疗目的作了讨论;展望了以EMCT作为早期胃癌经内镜根治疗法手段之一的远景。     

Peptide aptamers: exchange of the thioredoxin-A scaffold by alternative platform proteins and its influence on target protein binding

Peptide aptamers have emerged as powerful new tools for molecular medicine. They can specifically bind to and functionally inactivate a given target molecule under intracellular conditions. Typically, peptide aptamers are generated by screening a randomized peptide expression library, displayed from the Escherichia coli thioredoxin A (TrxA) protein. Here, we transferred peptide moieties from defined TrxA-based peptide aptamers to alternative scaffold proteins, such as the green fluorescent protein and staphylococcal nuclease. Yeast and mammalian two-hybrid assays as well as in vitro binding analyses show that the TrxA scaffold can be a major determinant for the binding of peptide aptamers. In addition, we demonstrate that TrxA can correctly display peptide sequences that correspond to the binding domains of natural interaction partners. Therefore, sequence analyses of TrxA-based peptide aptamers, isolated by two-hybrid screening from randomized expression libraries, should also be useful to find cellular binding partners for a given target protein, by homology. Received 1 August 2002; received after revision 17 September 2002; accepted 19 September 2002 RID="*" ID="*"Corresponding author.     

Progress in non-viral gene delivery systems fabricated via supramolecular assembly

Gene delivery systems are one of key issues that limit the development of gene therapy. The novel non-viral gene delivery systems fabricated via supramolecular assembly have begun to show increasing promising and applications in gene therapy due to its suitable nanometric size,controllable structure and excellent biocompatibility. In this review, the fundamental and recent progress of non-viral gene supramolecular assembly is reviewed. Artificial viruses the future direction of non-viral gene delivery systems are also described.     

多义的魅力--《白象似的群山》叙述技巧分析

作为海明威短篇小说经典的《白象似的群山》情节虽然简单,却充分体现了作者“冰山”文风的特点,在简单的情节之下蕴涵了丰富的意义空间。但评论界对这篇小说的理解普遍带有道德主义的倾向,认为它是一个可怕的故事。本文从小说的叙事方式、人物的叙事话语以及叙事视角等叙述技巧出发,挖掘了小说文本所创造的多义空间,对以往批评界对小说的纯道德评价进行了反驳。     

Investigation of hrDNA targeting vector-mediated tumor-specific suicide gene therapy for hepatocellular carcinoma

Vectors pose most pivotal problem of gene therapy[1]. Because of the high transfection efficiency both in vitro and in vivo, the viral vector has been employed in 70% clinical trials of gene therapy (http://www.wiley.co.uk/ genmed/clinical). However, thei…     

BNCT物理剂量Monte Carlo程序系统——MCDB及算法

硼中子俘获治疗(BNCT,boron neutron capture therapy)规划软件系统MCDB(Monte Carlo dosimetry inbrain),包括医学前处理、Monte Carlo物理剂量计算和后处理。采用中心点方法确定网格的材料和密度,并自动生成Monte Carlo输入文件。MCDB借鉴并发展了一套网格几何下的快速粒子径迹算法,取得了与MCNP程序一致的剂量计算结果,计算速度较MCNP程序提高2.7~3.5倍。MCDB可进行并行计算,具有线性加速比,能够满足BNCT临床对计算时间和精度的要求。     

《左传》叙事艺术三题

《左传》的叙事艺术表现在三个方面 :(1)伏笔艺术。以小事作大事的伏笔 ,揭示大事发生的根源 ;(2 )论断艺术。在叙事中寄寓作者对历史事件或人物的评价 ;(3)跳叙艺术。故事情节大胆跳跃 ,使叙事更精练 ,关键环节更突出     

叙事的转喻--读森子的诗

评述了当代诗人森子20世纪90年代以来的诗歌写作。森子的诗歌在经历了 从抒情的主体性向修辞性的转变之后，又从反讽式的修辞转向叙事，通过对日常生活的叙述 ，使它所指述的现实发生转义。