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61.
摘要: 目的探讨创伤对机体脂质过氧化及抗氧化能力的影响。方法选择普通级新西兰兔11 只采集血样,采集后双侧耳缘小静脉反复穿刺,隔日1 次,连续2-3 次,建成创伤模型后采集血样,检测建模前后2 组血清丙二醛( MDA) 、超氧化物歧化酶( SOD) 、过氧化氢酶( CAT) 、谷胱甘肽过氧化物酶( GSH-Px) 、总抗氧化能力( T-AOC) 指标,并予以统计分析。结果MDA: A 组( 3. 048 ± 1. 259) nmol /L,B 组( 3. 251 ± 1. 695) nmol /L,A 组水平低于B 组;T-SOD: A 组( 384. 314 ± 50. 122) U/mL,B 组( 348. 942 ± 45. 852) U/mL,A 组水平高于B 组; CAT: A 组( 6. 121 ±4. 097) U/mL,B 组( 4. 563 ± 2. 223 ) U/mL,A 组水平高于B 组; GSH-Px: A 组( 377. 808 ± 72. 726 ) U/mL,B 组( 349. 942 ± 54. 021) U/mL,A 组水平高于B 组; T-AOC: A 组( 5. 537 ± 2. 128) U/mL,B 组( 4. 633 ± 2. 074) U/mL,A组水平高于B 组; 组间差异均无统计学意义( P > 0. 05) 。结论创伤对机体脂质过氧化及抗氧化能力具有一定影响,但无显著性影响。 相似文献
62.
CHENG WeiXuan LIANG XuFang SHEN Dan ZHOU Qiong HE Yan HE Shan LI GuangYu 《科学通报(英文版)》2012,57(6):615-622
Liver glutathione S-transferase(GST) plays a major role in the detoxification of microcystins(MCs) via conjugation to glutathione(GSH).We evaluated the relationship between seasonal variation in fish gut contents and the expression of GST isoforms in mud carp(Cirrhina molitorella) and Nile tilapia(Oreochromis niloticus).We quantified the abundance and diversity of plankton in the water column and foregut of mud carp and Nile tilapia in the tropical Xiangang Reservoir between October 2007 and July 2008.The mRNA expression of 7 liver GST isoforms was determined by real-time RT-PCR.The gut contents of both species were dependent on the amount and type of phytoplankton and zooplankton in the water.The expression of liver GST genes in Nile tilapia and mud carp was positively and negatively correlated,respectively,with the abundance of toxic cyanobacteria in the fore-gut.The expression of liver GST mRNA was correlated to the abundance of toxic cyanobacteria in the gut contents of both species,suggesting that mRNA expression of GST isoforms could be used as a biomarker in Nile tilapia and mud carp to monitor cyanobacteria blooms in reservoirs. 相似文献
63.
谷胱甘肽(GSH)是一种普遍存在的生物硫醇,具有清除毒素、维持氧化还原稳态和调控基因的作用,GSH的异常水平可能是多种疾病的触发因素.过渡金属氧化物二氧化锰(MnO_2)具有很强的氧化能力,能被GSH还原为Mn~(2+),可用于磁共振成像(MRI)以及肿瘤治疗.GSH和MnO_2之间的氧化还原反应已成为科研工作者不断研究和探索的方向.综述了GSH和MnO_2的氧化还原反应的最新研究进展. 相似文献
64.
Summary In rat intestine, the glutathione level was increased, glutathione peroxidase activity decreased and glutathione-S-transferase unchanged by vitamin A deficiency. 相似文献
65.
Melatonin regulation of antioxidant enzyme gene expression 总被引:15,自引:0,他引:15
Mayo JC Sainz RM Antoli I Herrera F Martin V Rodriguez C 《Cellular and molecular life sciences : CMLS》2002,59(10):1706-1713
Antioxidant enzymes (AOEs) are part of the primary cellular defense against free radicals induced by toxins and/or spontaneously
formed in cells. Melatonin (MLT) has received much attention in recent years due to its direct free radical scavenging and
antioxidant properties. In the present work we report that MLT, at physiological serum concentrations (≈ 1 nM), increases
the mRNA of both superoxide dismutases (SODs) and glutathione peroxidase (GPx) in two neuronal cell lines. The MLT effect
on both SODs and GPx mRNA was mediated by a de novo synthesized protein. MLT alters mRNA stability for Cu-Zn SOD and GPx.
Experiments with a short time treatment (pulse action) of MLT suggest that the regulation of AOE gene expression is likely
to be receptor mediated, because 1-h treatment with MLT results in the same response as a 24-h treatment.
Received 18 June 2002; received after revision 5 August 2002; accepted 27 August 2002
RID="*"
ID="*"Corresponding author. 相似文献
66.
Salvi M Toninello A Schweizer M Friess SD Richter C 《Cellular and molecular life sciences : CMLS》2002,59(7):1190-1197
Mitochondria contain a specific Ca2+ release pathway which operates when oxidized mitochondrial pyridine nucleotides are hydrolyzed. NAD+ hydrolysis and therefore Ca2+ release is possible when some vicinal thiols are cross-linked. Here we report that the thiol oxidant peroxovanadate inhibits
the specific Ca2+ release pathway. In mitochondria, peroxovanadate causes a complete loss of reduced glutathione, which is not accompanied
by formation of glutathione disulfide, and a partial loss of protein thiols. In model reactions, peroxovanadate oxidizes reduced
glutathione predominantly to the sulfonate derivative, but does not react with glutathione disulfide. When the vicinal thiols
relevant for Ca2+ release are cross-linked, Ca2+ release is no longer inhibited by peroxovanadate. Conversely, pretreatment of mitochondria with peroxovanadate makes them
insensitive to compounds promoting the disulfide state. These results suggest that peroxovanadate inhibits the prooxidant-induced
Ca2+ release from mitochondria by (i) depleting mitochondria of reduced glutathione and (ii) oxidizing the vicinal thiols relevant
for Ca2+ release to a state higher than disulfide, presumably the sulfonate state. The findings provide further insight into the regulation
of Ca2+ release from intact mitochondria, and may be relevant for a better understanding of the action of peroxovanadate in cells,
where the compound can be insulin mimetic.
Received 28 March 2002; received after revision 8 May 2002; accepted 15 May 2002 相似文献
67.
通过简便的两步阴离子交换色谱,从遗传工程菌株大肠杆菌JM105中纯化出哺乳动物的谷胱甘肽S-转移酶5-5(GST5-5),GST5-5以二卤甲烷为底物时的动力学常数,热稳定性和最适pH被测定,并与细菌二氯甲烷脱卤素酶的上述性质进行了比较。 相似文献
68.
以10月龄BALB/C雄性小鼠为实验对象,研究珍珠口服液对衰老指标的影响,结果表明:与对照组相比,珍珠口服液使脑B型单胺氧化酶比活性下降1899%(P<0001);红细胞谷胱甘肽过氧化物酶活性升高1883%(P<0001);心肌脂褐素含量下降2433%(P<001),表明珍珠口服液具有一定的延缓衰老的作用。 相似文献
69.
人体内的超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-PX).是清除和OH、ROOH等自由基的主要酶.通过测定正常人、麻风病人的血液SOD、GSH-PX活性.探讨麻风病人血液SOD、GSH-PX变化对自由基清除的影响.结果显示:麻风病人体内的SOD和GSH-PX活性显著低于正常人(P<0.01);而现症和治愈、多菌型和少菌型、活动期和消退期病人之间无显著性差异(P>0.05).提示SOD和GSH-PX活性降低与麻风杆菌感染有关.且感染麻风菌后病人治愈前后血液中的SOD和GSH-PX活性没有明显变化. 相似文献
70.
The tissue-specific expression of glutathione S-transferases (GSTs) in the cotton bollworm and the expression level induced by 2-tridecanone and quercetin were examined using the methods of biochemistry and the quantitative PCR. The relative expression level of GST mRNA was unanimous with the GSTs activity conjugaging with 1-chloro-2, 4-dimitro-benzene (CDNB) in fat bodies,midguts, heads and integuments of cotton bollworms. The GSTs activity in fat bodies was the highest, then midguts, heads and integuments in turn, which was in consistent with the relative expression level of GST mRNA. The specific activity of GSTs and the relative expression level of GST mRNA could be significantly induced by 2-tridecanone and quercetin, and after the induction the order of the GSTs activity and the relative expression level of GST mRNA in the above four tissues in cotton bollworms was not different from the control.The induction of GSTs by 2-tridecanone was stronger than by quercetin in all four tissues, which was in accordance with the relative expression level of GST mRNA. It suggested that the increase of GSTs activity induced by plant allelochemicals was associated with the elevated expression of GST mRNA in cotton bollworms. 相似文献